UDK 903’12/’15(46)“633/634”:314.18 Documenta Praehistorica XXXI Building a method for the study of the Mesolithic- Neolithic transition in Portugal Mary Jackes1, Christopher Meiklejohn2 1 Department of Anthropology, University of Alberta, Edmonton, Canada mjackes@ualberta.ca 2 Department of Anthropology, University of Winnipeg, Winnipeg, Canada c.meiklejohn@uwinnipeg.ca ABSTRACT – This paper focuses on the agricultural transition in Portugal and on demography across this transition, concentrating on two key skeletal samples, the Mesolithic shell midden of Cabeço da Arruda and the Neolithic burial cave of Casa da Moura. It extends our previous work on the demography of the transition and the methodology surrounding its determination. We explain our method for determination of the number of individuals in samples where whole skeletons cannot be used. We then concentrate on the estimation of fertility, placing it within limits of biological feasibility, sample inadequacies, and vagaries of age assessment. From our analysis, which includes an examination of historical issues with the sites, we argue for regional population continuity between 8000 and 6000 calBP, and suggest that Neolithic life-ways slowly intensified, founded on important elements deriving from the late Mesolithic, with changes that included increased fertility through shortening of the birth interval. IZVLEEK – V lanku se osredotoamo na prehod v kmetovanje na Portugalskem in na demografijo pri tem prehodu, e posebej na dva kljuna vzorca skeletov – mezolitsko najdie koljnih lupin Cabeço da Arruda in neolitska jama s pokopi Casa da Moura. To je nadaljevanje naih dosedanjih raziskav demografije prehoda in s tem povezano metodologijo. V lanku razloimo nao metodo za doloevanje tevila posameznikov v vzorcih, kjer ni mogoe uporabiti celih skeletov. Nato se osredoto imo na oceno rodnosti glede na bioloko zmonost, neustreznost vzorcev in glede na omejitve pri ocenah starosti. Na osnovi naih analiz, ki vkljuujejo tudi zgodovinske vidike raziskav najdi, zagovarjamo regionalno kontinuiteto prebivalstva med 8000 in 6000 calBP. Menimo, da se je neolitski nain ivljenja poasi intenziviral na temelju pomembnih elementov iz poznega mezolitika, spremembe pa so vkljuevale naraanje rodnosti in skrajevanje asovnega razmika med rojstvi. KEY WORDS – Mesolithic; palaeodemography; human skeletons; Muge The dynamics of the agricultural transition consti-change in the nature of population growth. We will tutes one of the most debated areas of Holocene Old explore this concept using skeletal samples from World archaeology (see e.g. Ammermann and Biagi Portugal that span the transition. In particular, we 2003; Price 2000). In this paper we will focus on will investigate the large samples of apparently priwork in Europe and examine the use of human ske-mary burials excavated at the Mesolithic midden letal material, which is critical to any discussion on sites of the Muge River valley, as compared with the a demographic transition and population growth equally large but quite differently interred Neolithic contemporaneous with the agricultural transition. skeletal samples from sites such as the ossuary cave of Casa da Moura in the Estremadura. We will ex- Bocquet-Appel (2002) argues that there was a Meso-plore, in more depth than previously, the determinalithic/Neolithic transition in Europe entailing a major tion of numbers of individuals found within these Mary Jackes, Christopher Meiklejohn sites, an issue which is crucial to their use in demographic analyses. As part of this discussion we will use published and unpublished data to examine the history of excavation of the Muge sites, focussing on Cabeço da Arruda, among the most complex of any Mesolithic site in Europe, with a history of multiple excavations going back to the 1860s. SKELETAL SAMPLES OF THE PORTUGUESE MESOLITHIC/NEOLITHIC TRANSITION AND THEIR CONTEXT – INTRODUCTION The sites that will be discussed in this paper in varying degrees of detail are in central and southern Portugal (Fig. 1). The two Mesolithic sites, Cabeço da Arruda (henceforth Arruda) and Moita do Sebastiao (henceforth Moita), are near the exit of the River Muge into the Tagus, about 30 km northeast of the current Tagus estuary. These sites have had the most complex excavation histories of any European Mesolithic burial sites. First explored between 1863 and 1865 by A. F. Pereira da Costa and C. Ribeiro, they were excavated twice again in the 19th century, in 1880 by Ribeiro and again later in the 1880s by F. de Paula e Oliveira1. In the 20th century, excavations occurred in two phases. A. A. Mendes Correa excavated Arruda in 1937 with his collaborators, R. da Serpa Pinto, J. R. do Santos Junior and A. Ataíde, following several years of excavation in the early 1930s at Cabeço da Amoreira. In 1951 the bulldozing of the Moita site led to intervention by Mendes Correa and excavation from 1952 to 1954 by J. Roche and O. da Veiga Ferreira. Following upon this, excavations were undertaken at Cabeço da Amoreira between 1958 and 1967 under the direction of Roche and Veiga Ferreira, and extended to Arruda in 1964 and 1965. Finally, new excavations at Amoreira have begun under the direction of J. M. Rolao and M. Roksandi, with some section cleaning and stabilization at Arruda. Our primary discussion in this paper is of Arruda, while papers on Moita are in preparation (Alvim, Jackes in prep.; Jackes, Meiklejohn in prep.). The major Neolithic site is Casa da Moura, a burial cave in the Estremadura, a karstic region north of Lisbon. It was first excavated in the 1860’s by Delgado (1867) and re-examined by L. G. Straus in the Fig. 1. Central and southern Portugal showing the location of a number of important Mesolithic and Neolithic sites. 1980’s (Straus et al. 1988). Other sites noted in Figure 1 will be discussed where relevant. The three surviving midden sites of the lower Muge valley lie on either side of a broad area of marsh through which a channel for the river has been dredged (Fig. 2). Moita and Amoreira lie just over 1 km apart on the south side of the valley, with Arruda just over 2 km to the northeast of Amoreira on the north side of the valley. Arruda lies on the edge of the valley flood plain and has been impacted by flooding, while Moita now lies under a rice processing installation2. Work in the 1950s and 1960s produced the first absolute dates for the Muge series, based on charcoal. These were from the Saclay laboratory, very early in the history of 14C dating, with standard errors of 300 to 350 years. At Moita they provided a date for the base of the sequence (layer II) of 7080 bp3 (Roche 1957). At Arruda they provided bracketing dates 1 See, e.g., Cardoso and Rolao 1999/2000, Newell et al. 1979 and Roche 1972. Roche was incorrect in stating that an 1892 excavation took place (Roche 1972b.75). 2 The lower Muge Valley is now structured as paddies for the intensive cultivation of rice. Other shell middens were destroyed in the 19th century for the planting of vines. 3 Uncalibrated dates are expressed as bp, while calibrated dates are expressed as calBP. Building a method for the study of the Mesolithic-Neolithic transition in Portugal Fig. 2. The classic Muge Mesolithic sites (after Ro- che 1972.20.Fig. 2). The Muge is an area of marsh (stippled) through which a drainage canal has been dredged. for the base and top of the site of 6430 to 5150 bp (Roche 1965a; 1965b). At Amoreira there were also bracketing dates of 7030 to 6050 bp (ibid.). Any overall chronology of the sites based on these dates was enigmatic at best and none of these dates could be firmly linked to any of the human skeletal material. The best date for Moita was 7080±130 bp for basal breccia charcoal (H2119/1546: Roche, Veiga Ferreira 1972/73) and for Amoreira, also on charcoal, 7135±65 bp (Hv–1349: Soares, Cabral 1984; Kalb 1989). No good dates were available for Arruda. As part of our re-examination of the Muge skeletal series in the 1980s the authors, working with David Lubell, instituted a program of new AMS 14C dates directly on the human skeletal material (Lubell et al. 1994) to which new dates have been added, deriving for example from the most recent excavations at Arruda by Rolao and Roksandi (Roksandi in press). The overall results (Fig. 3) suggest a sequential chronology, though the actual occupation of the sites may overlap. Moita seems to be the earliest with dates covering the period 7240 to 6810 bp. Amoreira with dates from 6630 to 6550 bp seems younger but needs further work. Arruda seems to fit in between, with dates of 7040 to 6360 bp. The more completely dated sites suggest an approximate 400 year time span for the occupation of Moita and a slightly longer 600 years for the occupation at Arruda. The possible sequential occupation of these sites is important in terms of other data that suggest differences between the sites, both in terms of the environment and human biology (for biological variability see Jackes, Lubell 1999). There may be evidence of a change in patterns of environmental exploitation (Fig. 4, Lentacker 1991). The graphed data are for terrestrial herbivores on the three Muge sites, derived from the representation of the relevant taxa4. The frequency of lagomorphs apparently increases between Moita and the later sites. Suids, cervids and bovids all decrease, which could involve several variables including environment and access to animals within the effective catchment zone of the sites. Some specific non-food related faunal elements may provide other clues: red squirrel (Sciurus vulgaris) bones at Arruda indicates the presence of pine forest, as opposed to the current dominance of oak (Antunes 1985). Support for increased pine in the vicinity around 7500 calBP comes from the work of van der Schriek (in prep.; van der Schriek et al. 2003), which also documents this period as the time of maximum tidewater influence in the Muge. Thus, there were environmental changes over what appears to be a quite restricted time period of the Mesolithic occupation at Muge. Resource changes would have been mediated especially by the rise and fall of the Holocene sea level resulting in a rise and fall of the important molluscan dietary sources, especially Scrobicularia plana. While faunal data may reflect a shift in diet within the period of occupation at the Muge midden complex, we have independently studied this through use of stable isotope determinations of 13C and 15N (Lubell et al. 1994). Results for Moita and Arruda, together with other Mesolithic individuals, and a Neolithic series, are seen in Figure 5. The results show a trend away from marine dietary elements towards terrestrial elements. As a series, Moita appears to be more marine than Arruda and both appear to be more marine based than the majority of the Neolithic individuals. There is no major divide in the sequence comparable to the marked shift documented for Danish material by Tauber (1981; see also Meik 4 Material is from 1930s excavations at Arruda and Amoreira, but material for Moita may be from the 1960s (Lentaker pers. comm. 8.iii.2004), so there are certainly sampling problems related to the multiple excavations. A rereading of multiple reports on Moita excavations suggests the possibility that the layers containing mammalian bone were removed by bulldozers prior to excavation at Moita in the 1950s. Mary Jackes, Christopher Meiklejohn lejohn et al. 1998). However, the full situation is quite complicated when sample variation and outliers are examined. How do we explain outliers? Among the Mesolithic individuals we have one with an extremely high 15N value (>16), Samouquiera H2, who consisted only of arms when excavated (Lubell, Jackes 1985). The individual showed bilateral arthritis in wrists and hands, especially marked on the right side. The body was buried close to Samouqueira H1 who had sustained a major fracture of the right humerus leading to an open infected wound and also had an infected wound of the right foot. Both individuals therefore could be suggested to have disabling trauma, a consequence of which is likely to be a way of life and a diet different from their Mesolithic fellows. In addition, there may be a form of burial different from that for able-bodied people. In the case of Samouqueira, we seem to have people who, in the months before their deaths, were separated from the rest of the population. No other human remains were found among the 286 faunal bones5 (mostly Oryctolagus cunniculus) scattered among the marine bivalves, gastropods and fish covering this cliff-top site. In the Neolithic sample we have an individual who shows the most extreme marine shift in 13C values in our sample. The individual, who can be said to be maintaining a Mesolithic style diet, is our only analyzed individual from the Melides cave of Lagares, demonstrating how much more work needs to be done before we really understand the Mesolithic/ Neolithic transition. This individual also has a high 15N content in the single stray rib analyzed6. One Fig. 3. Calibrated AMS 14C dates directly on the human skeletal material (Lubell et al. 1994). New dates, identified as “top” and “base”, from recent excavations at Amoreira and Arruda by Rolao and Roksandi (Roksandi in press). 1 and 2 standard deviations are shown beneath the probability curves (Bronk Ramsay 2002). Fig. 4. Percentage representation among selected terrestrial mammals in the Muge collections of the Sala de Arqueologia e Pré-História Mendes Correa Museu de História Natural, Faculdade de Ciencias Universidade do Porto (Lentaker 1991.254–255). The Moita material is, however, in the museum of the Serviços Geológicos de Portugal. 5 The exact number provided by Lentacker (pers. comm. 19. IX. 1986). 6 Since the site was an ossuary cave of disarticulated bone, we can say nothing more about this individual. Further analyses are now being undertaken on material from this cave. Building a method for the study of the Mesolithic-Neolithic transition in Portugal Fig. 5. Stable isotope values for Portugal. “Other Mesolithic” sites refers to Amoreira and to Samo- uqueira in the outlier position at the extreme ma- rine pole of the diagram. Arruda individual (N) is an outlier for the Arruda stable isotope distribution (Fig. 6), with a more marine based diet, and we know that he is exceptional for the Mesolithic population, with spinal changes that included osteoporotic collapse of T.10 and collapse and fusion of L.1 and L.2. In the discussion above we proposed an initial interpretation from Figure 5 of a gradual shift from a marine to a terrestrial based diet. The conclusion is problematic when outliers are considered and especially when the dates for specific individuals are examined from the site of Arruda (Fig. 6). At the Neolithic “pole” to the left, marked by the most terrestrial shift, are Arruda A and D, individuals falling within the middle of the dated range for Arruda specimens (Fig. 7: individuals with probability ranges in grey)7. At the opposite Mesolithic “pole”, the most marine- shifted, are Arruda N and an undated sample from 20th century excavations now being analyzed by Eugenia Cunha, University of Coimbra. Interestingly, Arruda N provided the youngest date in our Arruda time series at 6360 bp. As a result, there is evidence contrary to a clear temporal trend within Arruda, the site which may provide our longest occupation span. On the other hand, a possible interpretation of Arruda N stable isotopes would focus Fig. 6. Arruda stable isotopes. The two skeletons with “e” designations are undated individuals now being studied at the University of Coimbra. The skeletons labelled “top” and “base” are from recent work at Arruda by Rolao and Roksandi (Roksandi in press). on the date as illustrating a trend towards the reduction of tidewater influence in the Muge, and thus a reduction of estuarine resources. There may have been pressures for seasonal travel far down the Tagus River. This interpretation of Arruda N depends on a manipulation of the date of this individual. Critical to the entire question of Muge Mesolithic dating is whether the reservoir effect is skewing the dates. The date alteration for a 100 percent marine diet entails an age offset of 253±29 years for Portugal (Monge Soares 1993). The results of calibration with a reservoir effect correction estimated from the stable isotope values for each individual are seen in Figure 8. At the top of the diagram we have dates estimated for individuals (like Arruda N) with stable isotope values indicating a large marine component in the diet. At Fig. 7. Arruda dates (Lubell et al. 1994; Roksandi in press). The proba- bility ranges in grey are individuals with low 13C values. 7 A single further Arruda date with –19 13C has been published (Cunha et al. 2003.185) which suggests that this pattern was established 600–800 years earlier, although the other Muge dates also published here are more congruent with previous information. Mary Jackes, Christopher Meiklejohn the bottom are dates calibrated without a marine shift, because the dated samples are charcoal. Thus, from bottom to top of the diagram, we have increasing degrees of marine correction based on stable isotope figures, within the four groupings, showing no reservoir effect and marine corrections for 25 percent, 50 percent and 75 percent marine diet respectively (these are no more than rough estimates of the percentage of marine dietary component). The dates on human bone are from our own database, with the analyses undertaken by Henry Schwarcz (McMaster University) and by Isotrace (University of Toronto). No early Saclay dates are shown. It is of interest that, using reservoir effect corrections, Arruda N (together with Samouqueira, TO–130 6370±70 bp) is found to be a little younger than Mesolithic dates from Vidigal (Gx–14557 6030±180 bp charcoal) and Fiais (ICEN–141 6180±110 bp faunal bone), and equivalent in age to TO–953 Fig. 8. Human bone from Muge Mesolithic middens with dates recali(5990±60 bp, a human bone brated by mixing curves based on estimates from the stable isotope date from Casa da Moura), when data for the same individuals. With 50% the intcal 98 and marine 98 curves are mixed equally. The material with 0% marine resources con- the latter is calibrated with a 25 sidered in the date calibration are charcoal samples: Moita H2119/ percent marine correction8. All 1546 basal breccia level (Roche, Veiga Ferreira 1972/73); Amoreira these five dates calibrate as Hv–1349, basal level 39 (Soares, Cabral 1984; Kalb 1989). younger than the earliest Portuguese Neolithic date, Oxa–1033 (6130±90 bp on from the site of Arruda be used to develop a me- Ovis bone from Caldeirao). The conclusion is that thod for examination of the demography of the Mean assumed gap in time and stable isotope values solithic/Neolithic transition? We look at the history between the Mesolithic and Neolithic in Portugal of the site and then at the extant skeletal sample may be an artifact derived from incomplete analyses. that we have been working with for twenty years. The site of Arruda is a large, roughly oval, midden THE ARRUDA SKELETAL SAMPLE AS A BASE on the north side of Muge River valley. At the edge FOR DISCUSSION OF THE MESOLITHIC/NEOLI-of the valley flood plain, it was 95 by 40 m, with a THIC TRANSITION maximum depth of deposits of 5 m (Pereira da Cos ta 1865) or 100 by 60 m, with about 7 m depth of With the above discussion demonstrating Mesoli-deposit (Ribeiro 1880). The excavations by Pereira thic heterogeneity and the difficulty of pinpointing da Costa in 1863–1864, and by Roche and Veiga a “moment of transition” to the Neolithic, we turn Ferreira in 1964 and 1965, identified four Mesolithic to the more specific question raised at the begin-phases below the plough zone and disturbed soil, ning of the paper, can the human skeletal data from top to bottom, levels A to D. Only half the mid 8 Information on radiocarbon dates cited can be found at http://intarch.ac.uk/antiquity/jackes/dates.html Building a method for the study of the Mesolithic-Neolithic transition in Portugal den has been excavated to date (Fig. 9), and the skeletons from that half have an extremely convoluted excavation and curation history. The initial excavation of the site, by F.A. Pereira da Costa of the Serviços Geológicos de Portugal, followed the discovery of several shell middens along the Muge by Carlos Ribeiro in 1863. This excavation apparently unearthed around 45 individuals mostly recovered from the base of the site in level D (Pereira da Costa 1865.7,13) (Fig. 10). It appears that the material from the original excavations was destroyed by fire in the last quarter of the 20th cen tury9. However, even in the late 1920s Vallois could not find the first skull, that of a male, from the 1860s excavations, and considered it likely that it was already lost in the 1880s (Vallois 1930.353). He found only four other Arruda skulls in the Zoological Museum (ibid. 356) and it appears that he found no post-cranial remains whatsoever. A number of the finds were illustrated in the original publication (Pereira da Costa 1865) and we can confirm that the illustrated mandibles are not now in the Serviços Geológicos museum (Fig. 11). Studies of Arruda dentitions used only material in the Serviços Geológicos museum (Sueiro, Frazao 1959; LeFevre 1972)10. There is then a gap of fifteen years until the reopening of the site in 1880 associated with the International Prehistoric Anthropology and Archaeology Congress that was held in Lisbon in that year. Ribeiro opened up a further area of the site in order to provide a field trip for attendees at the Congress. This excavation again made it clear that the burials were not randomly distributed across the site (Fig. 12) but were grouped in the southwest part of the mound (Paula e Oliviera 1889.74). The skeletal material again came from a single level, presumably Fig. 9. Plan of Arruda, illustrating that about half of the midden has been excavated (modified after Cardoso, Rolao 1999/2000. Fig. 56). Fig. 10. Cross section of 1865 Arruda excavation showing localization of skeletons in one section of the basal layer of the midden – layer D on the lower right. The section goes from the north west (left) to the south east (right) and was first published (without a scale) by Pereira da Costa (1865.6.Fig. 2). 9 The material was stored at the Escola Politécnica which became the Faculty of Sciences of the University of Lisbon. On 18th March, 1978 the Museu e Laboratório Zoológico e Antropológico da Universidade de Lisboa (Museu Bocage) was destroyed by fire. 10 This was true even though Sueiro had assisted Vallois in searching for material at the Faculty of Sciences museum where he worked in the 1920s (Vallois 1930.339). Mary Jackes, Christopher Meiklejohn the same level as the material recovered in 1865 (Fig.13, level D). In addition, Ribeiro suggested that the skeletons were roughly aligned (Ribeiro 1880.286). Pereira da Costa (1865.13) had in fact suggested that the skulls were oriented to the NW). The number of skeletons recovered at Arruda in 1880 is unclear: over 120 individuals were said to have been recovered jointly from Arruda and Moita (Ribeiro 1880. 285). Again in the mid 1880s more excavations were undertaken, this time by Francisco de Paula e Oliviera, Ribeiro having died in November 1882. Further remains were recovered, in June 1884 “13 human skeletons, some in (Paula e Oliviera 1889. 59). However, it is very truth very damaged, of individuals of various ages”, likely that none of the 1885 skeletons came from and in June 1885 “the remains of 39 individuals for Arruda. Paula e Oliviera indicated that “I barely got the most part in a very good state of preservation” one skeleton in bad shape” (Paula e Oliviera 1889. 59) from Arruda11. Later excavations also recovered far fewer remains. The remains associated with the excavations of the 1930s were deposited in Porto and when examined by the authors were not in a condition to permit study. Most were removed en bloc and had not been cleaned and prepared, but original reports suggested 11 individuals had been excavated. Roche and Veiga Ferreira recovered only 13 graves in 1964 and 1965 and the finds have not been studied. As a result, any discussion of the human skeletal material from Arruda is largely concerned Fig. 12. After a plot by Carlos Ribeiro of the Arruda midden excavations with finds recovered in the in 1880. We are shown the locations of skeletons among the piles of back 1880s. In the late nineteenth dirt. Another plot dated 15th April, 1880 detailing the location of 15 ske-century, it was believed that letons makes it clear that the points marking the skeletal finds here are the Arruda skeletons stored extremely approximate. This and a number of other records of the 1880 in the Muge collection in Lis- excavations were found in the archives at Serviços Geológicos de Portugal and copied by David Lubell, 20th June 1989. It is believed that this ma-bon numbered no more than terial has now been deposited in the archives of Instituto Geológico e Min-41 individuals (Hervé 1899. eiro, but has not been accessioned (Pedro Alvim pers. comm. 13. II. 2004). 267). Fig. 11. Some of the Arruda skeletal material excavated in 1865 (selections from Plate I and Plate II Pereira da Costa 1865). The illu- strated specimens are distinctive so that it can be confirmed that they are now lost. 11 A letter dated 4th June 1885 from Paula e Oliviera to Nery Delgado indicates that all the human material excavated in 1885 is likely to have come from Moita (Alvim pers. comm. 13.II.2004), neither Arruda nor Amoreira having produced results. For further discussion see Alvim and Jackes in prep. Building a method for the study of the Mesolithic-Neolithic transition in Portugal The study of Arruda has presented logistical problems for the authors of this paper. Neither the full site nor the associated skeletal remains have ever been published in a single definitive study comparable to that of Moita (Ferembach 1974; Roche 1972). Meiklejohn first inventoried the collection in 1969 as part of an earlier study (Meiklejohn 1974). We both studied the material in 1985, and Jackes reexamined the collection during work at the Serviços Geológicos that extended from 1986 to 1989. As part of the research reported here we have recently spent 3 months in a re-examination of the mandibles, using photographs taken by the two of us, records of detailed observations and measurements, radiographs and field notes. The major problem is that, although the material has been consistently curated in the same facility at the museum of the Serviços Geológicos in Lisbon, the material has been mixed. Extensive examination of the historical records shows three different potential bases for this. The earliest may relate to the original conditions of burial. Perhaps these were not always the single inhumation burials that are usually inferred, a question raised in the early publications, but it is most likely that the grouping of burials led to disturbance of earlier by later inhumations, to the extent that the original report spoke of skeletal elements found “pell-mell” and “in the most bizarre positions imaginable” (Pereira da Costa 1865.15). A second potential source of mixing is at the original time of excavation and subsequent deposition in museums. The 1880 excavations involved the exposure of the burials considerably prior to the International Congress, and participants at the Congress were permitted full access to the burials during the field trip, so mixture may have occurred at that time. The materials excavated in the 1880s were then subject to over 120 years of curation under circumstances such that mixture of material was almost inevitable. Individual bones were never labelled and the open drawer system (in place until we reorganized the material in closed boxes from 1984 to 1986) invited misplacement of bone. We thus have a situation where the material is curated as individual inhumations but many of these are not “individ uals”. One “individual” has 66 metatarsals, including 13 left second metatarsals and another includes nine right and six left clavicles12. Yet a third (“individual” M) has 41 separate maxillary and mandibular fragments actually representing 21 separate individuals. The photographs from Arruda in 1880 (e.g. Fig. 13) give a reasonable sense of the distribution of the finds within a single level and they also help us understand that there were problems made obvious by the following quotations: “One sees also bones here and there, placed in confused heaps as if they had been gathered together once the flesh was gone; but in the majority of cases they are in their natural articulations...” (Cartailhac 1886.57) and “Because the bones were generally grouped according to their natural articulations, it is possible, most often, to collect separately those of each individual...” (Paula e Oliviera 1889.72; our emphasis). The problems can also be seen in the plots from the 1964 excavation of Roche and Veiga Ferreira (Fig. 14). Whether the skeletons numbered 6, 7 and 9 in this diagram represent two or three people could only be confirmed by detailed study. The suite of problems stemming from the history of the site can be summarized as follows:  In the 1860s it was already obvious that there were “bones not belonging to the skeletons" (Pereira da Costa 1865.16,18) and that the complete ske- Fig. 13. One of two photographs of the 1880 Arruda skeletons known from various publications. This photograph appeared in 1908 (Anon. 1908). The view is towards the south east along the excavation face and across the skeletons marked schematically by dots in Figure 12. 12 These were unnumbered individuals, called TO and SH by our project. Mary Jackes, Christopher Meiklejohn letons had, mixed among them, other bones (ibid. 18). Since no material from the 1860s is extant, we can no longer judge the extent to which material was selectively retained.  However, it is clear from the descriptions that not all bones were kept in the 1880s: “...the number of human skeletons that were recovered, even leaving aside the bones which were abandoned because of their bad condition, is truly considerable.” (Paula e Oliviera 1889.71, our emphasis).  At the time of the study by Vallois there were 42 drawers of Arruda material in the Serviços Geológicos museum (these remained until the early 1980s), but parts of two or three individuals were found in some drawers (Vallois 1930). Mixing was already a problem. Vallois also stated that he could not find a skull, a female, which had been deposited in the Serviços Geológicos museum. Vallois saw 20 to 25 Arruda skulls and some further cranial fragments (ibid. 340). He also studied some postcranial material, writing that he hoped someone would undertake the work of trying to redress the errors and reconstruct the bones (ibid. 365). Vallois considered that there was no possibility of matching skulls with postcrania (ibid. 364).  The material recovered in the excavations of the 1930s was sent to the Instituto de Antropologia Men- des Correa, Universidade do Porto. We attempted, independently, to inventory these materials: Meiklejohn in 1969 and Jackes in 1984. Although 10 or 11 individuals were said to be present (Cardoso, Rolao 1999/2000. 172–179), fire and subsequent flooding in 1974 (Huet Baçelar pers. comm.: August 1984) have complicated matters. When inventoried in 1984, it was clear that labels had been lost: most material at the Mendes Correa Institute was from Moita so that it could be checked against an earlier Moita inventory (Ferembach 1974), and there was obvious confusion after the fire. Of four Arruda indi viduals inventoried in 1969, one child from 193313 and three adults from 1937 can be clearly recognized in the 1984 inventory. In 1969 the adults had had skulls and mandibles: the skulls and mandibles were not found in 1984. In addition, other material was found with Arruda labels in 1984, including a child aged about 7 and a child aged about 13, both with dentition. Other material was unlabelled, and it could have come from either Arruda or Amoreira14.  In 1964 and 1965 Roche and Veiga Ferreira excavated 13 “skeletons”, apparently from the basal levels of Arruda (Newell et al. 1979, based on conversations with Roche in Paris in 1978). These had been placed in a rented storeroom separate from the Serviços Geológicos premises, and there was apparently no recollection of this, either in 1969 or in the period 1983–1989. They were rediscovered in 2000, and therefore have not been examined in detail15.  By the 1980's, when we began our work at the Serviços Geológicos, there was obvious mixing among individuals in the drawers. There was also loss of identification due to mixture and loss of labels16. In some cases material from a single individual had been dispersed, some elements being kept Fig. 14. After a plot from the 1964 Arruda excavation of Roche and Veiga Ferreira (Cardoso, Rolao 1999/2000.Fig. 57:227). 13 A limited amount of work was done at Arruda in 1933 (Cardoso, Rolao 1999/2000.170). 14 Lentacker (pers. comm. 8.III.2004) confirms that problems caused by the fire were exacerbated when some of the faunal collection labels were destroyed by the water used to extinguish the fire. Meiklejohn worked on human material in a storage area which was later damaged by the fire. By 1984 this material was widely dispersed throughout the building. 15 All dental elements were photographed in detail by David Lubell (23 April, 2002). 16 Individual bones were never labelled with specimen numbers. Some, but not all, crania and mandibles were labelled. Detailed notes kept by the authors allowed us to track mixing which occurred during the visits of one or two other researchers even within the short period 1984–1986. Further mixing had occurred by April, 2002 (Lubell pers. comm.). Building a method for the study of the Mesolithic-Neolithic transition in Portugal in glass cases for display. The absence of any previously published inventory complicates any attempt to provide a catalogue raisonée. However, comparison with the similar problems in the Moita collection is illuminating. Ferembach prepared an apparently complete inventory of Moita prior to 1965 (Ferembach 1974). The same material was independently inventoried by Meiklejohn in 1969, without knowledge of Ferembach’s results. When both of us did a third inventory in 1984 and 1985, the discrepancies became apparent. While some of the descriptions were in agreement for all three inventories, all other possible agreements and discrepancies were observed, the most discrepant being totally different number associations in the three inventories. In addition it became clear that no inventory prior to 1984 had involved full cleaning of the material. We cleaned material in 1984 and 1985 that could not previously have been inventoried accurately. An example of this can be seen in the reported number of Moita teeth: Ferembach (1974) reports only 428 teeth, whereas preliminary estimates from the inventory of 1984 and 1985 list 889 teeth (Meiklejohn et al. 1988; Meiklejohn, Zvelebil 1991). ARRUDA AND CASA DA MOURA: HOW CAN WE COMPARE THEM? In studying the Portuguese Mesolithic/Neolithic transition we must compare Casa da Moura, a Neolithic ossuary burial cave (by definition a site with disarticulated and mixed individuals), with a Mesolithic site, traditionally regarded as having individual in- flesh burials. Even without the problems of Arruda, for which questions surround the burial practices, the excavations and the post-excavation history of the material, we would need careful consideration of methods in dealing with samples for demographic analysis. This is the core problem set up in the introduction to the paper and we will use mandibular counts as a basis for discussion of demographic questions at the Mesolithic to Neolithic transition. Palaeodemography demands exceptional care. In making statements based on inadequate and possibly incomplete samples, covering periods of time which are, at best, partially defined and generally too long for satisfactory demographic study, anything less than extreme caution is injudicious. To compare skeletal samples from different sites, it is necessary to use comparable methods of analysis for each site; methods of calculating the numbers of individuals must be comparable; methods of age assessment of both adults and subadults must be comparable, because different methods give different results (Jackes 1985). Consistency is critical. Taphonomic studies show that mandibles provide the highest number of elements in most skeletal collections, whether human or non-human. Use of mandibles allows for the maximum estimate of numbers in a site, different in many ways from the “minimum number of individuals” (MNI). Mandibles are more sensitive to details than other skeletal elements, carrying a great deal of information, and the use of mandibles also allows refitting even when clean reconstructable breaks are absent. Delgado (1867.46), in writing about Casa da Moura, recognized that the number of mandibles would give a much higher count of individuals than would the number of skulls, whole or fragmented, and especially the number of maxillae. But in sites where teeth are not retained in the alveoli, it is necessary to enquire also: 1. whether mandibular teeth are more often retained in the jaws and 2. whether mandibular teeth are more likely to be represented in the deposits than maxillary teeth, whether in situ in alveoli or loose. Neolithic sites contain many loose teeth and it becomes clear that more teeth are retained in the mandibles than in the maxillae. Figure 15, in which loose teeth are plotted as a percentage of total teeth, shows that fewer mandibular teeth are found loose in all tooth classes. As a result, this means that the lower teeth can be studied in more Fig. 15. Percent of loose teeth in Casa da Moura, compari- son of maxillary and mandibular dentitions (after Jackes, Lubell 1995.Fig. 8a). Mary Jackes, Christopher Meiklejohn detail, that their identification is more certain, and that fewer will be left unrecovered from the back dirt piles of an excavation. In fact, Delgado clearly stated that “maxillae are very rare while on the contrary mandibles are very abundant, and above all mandibular teeth…” (1867.46) and drew attention to parallel findings by Lartet with regard to non-human remains at Aurignac. This pattern is now well documented in vertebrate taphonomy. Furthermore, we have concentrated on molar teeth because, in Neolithic sites, up to 30 percent more molars are preserved than the next most common tooth types (premolars and canines). This is true of other Portuguese Neolithic sites that we have studied, besides Casa da Moura (and see e.g. Jackes, Lubell 1995. Fig 9 for comparison with a site studied independently, though using the same dental identification methodology). Figure 16 shows the ratio of the observed to the expected number of teeth based on the MNI for Casa da Moura. If all teeth for the total MNI were present, the ratio shown on the y axis would be 1.0: the second mandibular molar representation approaches an observed versus expected ratio of 1.0. Figure 16 reminds us that we need to look at more than the intact teeth that have been recovered and Fig. 16. Preservation of information on permanent mandi- bular teeth at Casa da Moura (after Jackes, Lubell 1995.Fig. 10). thus can be directly studied. We need to consider teeth that have been lost pre-mortem, from pathology or accident17; thus, the more alveolar sockets, the better. Unfortunately, such an approach means that the estimated number of older adults based on a sample partially made up of loose teeth will always be slightly wrong. The estimate of the ratio of subadults to adults may be too high for the Neolithic sites with many loose teeth, because information on premortem tooth loss may be gone. This must be emphasised – it is very possible that the number of older adults in Neolithic sites will be underestimated and the effect of this will be to give an apparent increase to the rate of fertility in the Neolithic in comparison with the Mesolithic. For Casa da Moura we obtain an MNI of 302 individuals with adult molars, derived from both the right lower M1 and the left lower M2. This is based on teeth with root development at least half completed or teeth fully erupted. Figure 17 provides a classic MNI type study for Arruda. This figure illustrates sockets so that we can examine not just intact teeth, but also both antemortem and postmortem tooth loss (in this case loose teeth are not involved, so that empty sockets can be taken into account). We can immediately see that the 42 skeletons that were assessed as the total number of all ages from infants to adults between 1880 and the late 1920s when Vallois (1930) looked at them, can- Fig. 17. Classic MNI study of Arruda mandibular sockets al- lows us to examine not just intact teeth, but also antemor- tem tooth loss and postmortem tooth loss. MNI based on Ar- ruda adult mandibles in the Serviços Geológicos, Lisbon 1984–1989. 17 Naturally, sockets that have lost teeth postmortem cannot be counted, since the shed teeth will be present in the deposits and must be assumed to have been counted. Building a method for the study of the Mesolithic-Neolithic transition in Portugal not encompass the full number of individuals represented in the Serviços Geológicos museum. The figure clearly indicates that the MNI for adults alone must be around 55, based on the value for RM1. However, the use of mandibles allows one to go beyond the classic MNI count for a site, since MNI depends on the side with the highest count. Our results are based on the reconciliation of three different inventories undertaken by the two of us, together and independently, from 1984 to 1989. Our records also include photographs, details of tooth status, attrition, pathology, measurements, notes and reviews of the refitting of mandibular fragments found in separate drawers and glass cases. We have obtained a remarkably consistent picture regarding the representation of sides and tooth types, based on 85 mandibular fragments aged 15 and above (including intact and fractured teeth, empty sockets, cases of premortem tooth loss and agenesis). And the use of mandibles clearly produces a result that is higher than the just derived MNI of 55 adults (Fig. 17). Primary work had in fact already shown us that 55 was not the maximum number of adult individuals. In 1986, 62 mandibles of individuals judged to be adults over age 15 were seriated (Figs. 18a, b, c). Furthermore, there were at least 8 other mandibles in the collection, mostly associated with skulls in the display cases in the museum. Of those under 15 years of age, 25 were initially seriated (Fig. 19), to which six were added from among the material on display in the museum. The estimate of subadults has some questionable individuals – perinatal infants who are often represented in the collection by long bones rather than mandibles. Our methodology takes account of these infants despite the absence of mandibles. But, in fact, the Fig. 19. Seriation of subadults based on eruption sequence and attrition. Fig. 18 a, b, c. Seriation of Arruda mandibles 15 years of age and older, based primarily on attri- tion with attention to pathology and preservation. This allowed reconstruction of separated mandi- bular fragments (some juveniles are shown in the centre of the images). demographic method we use ignores children under 5, because infants and young children are markedly under-represented in Neolithic skeletal collections, and are generally poorly preserved. Thus the uncertainty surrounding young children is not critical. The seriation of mandibular secondary teeth (Figs. 18 and 19) was based on observation of wear of the three lower molars, with secondary consideration of more anterior teeth. The initial seriation was visual, and undertaken by several people over a number of days, the placement of each fragment Mary Jackes, Christopher Meiklejohn being discussed. The same process had been previously undertaken on Moita adult mandibles. Arruda wear seems slightly reduced compared to what we had observed in the Moita series, and the Casa da Moura attrition is reduced from Arruda (see e.g. Lubell et al. 1994). However, the same sequence of attrition stages can be used: it covers eight wear levels, from 1, where the molar tooth has only just come into occlusion, to 8, with a rim on less than three sides, or all enamel removed and wear progressing down onto the tooth root (see Lubell et al. 1989 for details). Because Casa da Moura wear is slower than in the Mesolithic, more attention was paid to variations within wear levels. For example, wear level 3 lasted longer within a person’s adult life, and it was possible to discern gradations within level 3 that were not obvious in the Mesolithic, especially with Moita (Jackes 1992). Finally, the Arruda subadults were seriated separately (Fig. 19)18. The seriation process provided other benefits. Some identification problems were sorted out. In 1986 the process allowed the refitting of nine mandibles across drawers, that is, two or three fragments of each of nine mandibles were found dispersed over separate drawers. The drawer with label “M” was particularly problematic. It contained 13 maxilla fragments, 21 mandibular fragments and 27 loose teeth in 1984. There were actually 21 individuals represented by the M mandibular fragments. The R series identified and reported by LeFevre (1972) remains unidentifiable, but may have included material now labelled “M”. As noted above it was necessary to add further individuals to the visual seriation. These included mandibles in the display cases in Lisbon that could not be removed for study with the seriated specimens. Post facto seriation was possible though a variety of means that included photographs, measurements of the cemento-enamel junction height above the alveolar margin, and attrition scores. To the mandibles on museum display we can now add the material excavated by Roche and Veiga Ferreira in 1964, stored away and apparently forgotten. None of it was available in 1969 or in the 1980s. The dentitions were photographed by David Lubell and from those photographs attrition and status can be accurately recorded, at least to the level of comparison with material previously studied (Fig. 20). None of the 1964 mandibles match with any of the 1880s mandibles. This material represents eight new individuals, four under 18 and four over 18. Veiga Ferreira had actually plotted 13 individuals (Cardoso, Rolao 1999/ 2000.Fig. 57:227), but half of them were incomplete, so it is not surprising that we do not have 13 full individuals. The age of subadults is both very important and problematic. In the demographic approach used, it is important that we know which individuals are under 5 and which are 5 and over. Radiographs help with the question but a complete reassessment of dental age in Mesolithic and Neolithic Portuguese children is being undertaken because there are some questions about the timing of eruption sequence events. Figure 21 shows the type of information used in this study. Arruda M (41) is probably under 5 years of age, with the adult first molar unerupted, and the second premolar (P4) crown not yet visible below the 2nd deciduous molar. In Arruda 176, already 5 years of age, the first molar is advancing towards eruption, the premolar crowns are apparent, the second molar is forming, and the roots of the central incisors are developing. We arrive at a maximum estimate of 71 adults over 15 years of age present in 1984–1989 in the collection of the Serviços Geológicos, Lisbon. Adding subadults and the eight newly found individuals recovered from storage in 2000, the total is 105, based on mandibles. Fig. 20. Examples of mandibles from the 1964 Arruda excava- tions now in the Serviços Geológicos, Lisbon: Sk 17 8.2.311 on the right, Sk 18 on the left. Detailed colour photography (David Lubell) allows confirmation that none of this material was in the collections in the 1980s. 18 Examine the child still partially en bloc: in a North American site the interpretation would be that secondary bundle burialswere present. Building a method for the study of the Mesolithic-Neolithic transition in Portugal Our sample is shown in Table 1, with totals of 77 for Moita, 105 for Arruda and 340 for Casa da Moura. The previous publication of Casa da Moura (Meiklejohn et al. 1997) was based on an MNI of the loose right lower molars. Here we include a more complete set of data, including loose and in situ right lower molars, together with correction for premortem tooth loss (Jackes 1998; Arnaiz-Villena, Lubell 2000). The Moita sample includes those mandibles inventoried in Porto in 1984. There were a number of very young children in the Porto collection: the fact that they could not be seriated visually with the other Moita material is less important since our methodology ignores those under 5 years of age19. COMPARING ARRUDA, MOITA AND CASA DA MOURA The results of our study of the mandibles from Arruda, Moita, and Casa da Moura (Fig. 22) have implications for research on the demography of the Mesolithic/Neolithic transition. In order to provide a context, we have a large database of archaeological sites that have sample sizes of at least 10020. To that we have added historical data, for example some of the excellent French and French Canadian historical demographers’ analyses of parish records (e.g. Charbonneau 1970), allowing for an understanding of the biological realities underlying demographic data – something that has not always been considered by palaeodemographers (Jackes 1994). The plotted variables are the mean subadult mortality quotient (probability of death age 5–19, MCM or mean childhood mortality) and the ratio of children between 5 and 15 years to adults 25 and over. This approach to palaeodemography, the ratio of children to adults, was first suggested by Angel (1969), and has been systematized over a number of years through the work of Bocquet-Appel and Masset (e.g. Bocquet and Masset 1977). We use Bocquet-Appel’s index of juvenility, which we term the J:A. For further discussion of the approach see Jackes (e.g. 1992; 2000; Meiklejohn et al. 1997). To provide further external control we have used the model data of Coale and Demeny (1983) and the United Nations (1982). These data are marked on Figure 22 as representing populations that are Age Moita Arruda Casa Categories da Moura 0–4 14 17 42 5–9 6 9 31 10–14 1 5 33 15–19 3 4 18 20–24 8 8 64 25+ 45 62 152 Total 77 105 340 Tab. 1. Demographic data used in the analysis: age at death distributions. Fig. 21. Radiographs of juvenile dentitions: Arruda 176 (x-ray Sin- tra 90–2.VIII.86) and Arruda M(41) (x-ray Sintra PMD.1–5.XI.85). 19 Note that the Arruda material that was in the Mendes Correa Institute in Porto, now being studied by Eugénia Cunha at the Institute of Anthropology, University of Coimbra, is not included. This is because of questions arising from differences between the inventories of 1969 (Meiklejohn) and 1984 (Jackes and Huet Baçelar) and on what was destroyed in the 1974 fire/flood. A complete reassessment of Moita material is now being undertaken (Jackes and Meiklejohn in prep.), and it is to be noted that our publications on Moita have sample sizes varying from 77 to 79 in advance of this reassessment. 20 The Iron Gorge (Djerdap) samples of Jackes et al. in press are not included. Mary Jackes, Christopher Meiklejohn stationary, increasing or decreasing. The archaeological and historical data fall comfortably close to the model data: in those cases where the archaeological sites fall far from the expected, we can suspect or demonstrate sample problems (biases which might result from site chronology questions, age assessment error, partial excavation, preferential burial etc.). The diagram includes the full curve for populations that fall within the limits of reasonable demographic interpretation. As discussed elsewhere (Jackes 1994), the J:A ratio axis also provides a proxy measure for fertility. Populations at the upper right hand corner of Figure 22 would represent some of the highest recorded examples of natural human fertility. Some rare and exceptional archaeological populations do in fact provide calculated values beyond this apparent limit (e.g., Nea Nikomedia, Angel 1971). It seems likely that if they are to be interpreted as representing something other than samples unsuitable for demographic analysis by reason of their small size, they can be understood to illustrate exceptional circumstances. For example, one is a site in which there is ethno-historical evidence, not of high fertility, expected at the high end of the graph, but of high mortality associated with an influx of refugees from war and famine, preferentially of women and chil dren (Jackes 2000b). Another is of freed slaves returned to Liberia in the 19th century (1820–1843), a situation where young adults and children made up the bulk of the population (McDaniel 1992). The total fertility (henceforth TF) calculated for the Liberian emigrants from the US would provide values far beyond any possible human biological capacity for child-bearing, nearly 14 times higher than the actual Liberian TF rate at the end of the 20th century. In fact, the high end (at the upper right) of Figure 22 gives a total fertility estimate of about 12. The average woman in such a society would, between 15 and 45 years of age, have 12 live born children – an acceptable type of figure for maximum “natural fertility”. To assume that all women under a “natural fertility” regime21 will produce, say, 20 children is not reasonable: it is most likely to occur under unusual circumstances, for example, when an epidemic has killed all the children in a family and a “replacement family” is achieved. But it is not the rule for the average woman. Examples of immigrant populations that did not practice contraception and emphasized child bearing (e.g. Mormons and the Quaker immigrants to the north east coast of North America) never reached such figures. The best data on “natural fertility” comes from North American Hutterites early in the 20th century, suggesting 12 or 13 as the upper limit of TF. That the Hutterites had some limits on childbearing – some restrictions on sexual activity – is clear; nonetheless such an upper limit for TF is reasonable in the face of known social and biological constraints on human fertility. Coale’s Index of marital fertility is a basic demographic value by which the Hutterites of 1921 to 1930 are taken to represent the maximum potential level of childbearing within marriage, exceeded only under rare and unusual conditions. The figure given is 12.4 children. Fig. 22. Archaeological data, model data (Coale, Demeny 1983 West 1–10 increasing/decreasing/stationary and United Nations 1982) and historical samples provide the context for interpretation of information on the We must emphasize that we demography of Moita, Arruda and Casa da Moura. The Hutterite age at are talking here about the death distribution for the period 1941– 1950 (Eaton, Mayer 1953.238) number of children possible provides the test for the method. under some circumstances 21 One not practising contraception. Building a method for the study of the Mesolithic-Neolithic transition in Portugal and that – in order to make comparisons – we are using a theoretical figure that may overestimate actual fertility. It is important to note that not all women in a population will have 12 children and that such a value for TF is, in fact, highly unusual. We are discussing a demographic parameter, the average number of live born children per woman in a population. Not all women are equally fertile, foetal loss occurs, some husbands are sterile, not all women marry, not all marry young and are continuously sexually active from age 15 to age 45, not all women survive to menopause (see Jackes et al. in press; Jackes 1994 for discussion on the factors limiting fertility). Even French Fig. 23. Loess local regression fit line derived from model age at Canadian first settlers, few women death and fertility data (Coale, Demeny 1983 West 1–10 increasthough there were and with a strong ing/decreasing/stationary) demonstrates the placement of the es- incentive for large families, had a TF timated TF rates for the Portuguese archaeological sites. The fit of around only eight (derived from line is pegged at either end by test data: to the left !Dobe Kung (Ho- data in Charbonneau 1970). Much well 1979), at the right Hutterites dying in the period 1941–1950 of premodern western Europe, before (Eaton, Mayer 1953). antibiotics and with some crude contraception, relatively late marriage and with a less than 100% marriage rate, probably had a general population TF rate of well below eight. The Canadian Iroquoians were noted by the first Europeans who lived with them as having few children (Jackes 1994), perhaps five as a maximum (similar to Moita, and maybe to the total Iron Gates Mesolithic (see Jackes et al. in press)). Stationary and decreasing populations fall at the lower left (Fig. 22), with Moita just at the upper end of stationary limits. In contrast, Arruda has already gone beyond that, and Casa da Moura would be beyond that again, firmly within the group of increasing populations. The values derived from the Hutterite age at death distribution for the period 1941– 1950 (Eaton and Mayer 1953.238) fall close to Casa da Moura. Remember, however, that Casa da Moura, especially, has an inbuilt error within it. Because we do not have a full understanding of the number of old people who had lost their teeth premortem, because of the problem of loose teeth and destroyed mandibles, we must assume that there were actually more adults than we have shown here. Casa da Moura would probably fall a little lower on the curve, closer to 0.25 on the J:A (y) axis than its current position of ca 0.30. In Figure 23 we have a loess line (a local regression based fit line) derived from the fertility rates associated with the series of Coale and Demeny model tables we have used. The J:A ratio is again on the y axis. The x axis now represents the TF rate. On the line we have added two points shown in black. The point to the lower left represents Nancy Howell’s work on the Dobe !Kung (Howell 1979), with a TF estimate of just under four children derived from the J:A of the age at death distribution. This accords with Howell’s own estimate of 4.3 for the period from 1963–1973. Note the position of the Hutterite estimate of nine for the TF rate estimated by regression from the age at death distribution J:A for the period 1941–1950 (Eaton and Mayer 1953.238, ages redistributed into standard age units). Summing the age specific fertility rates of all Hutterite women of reproductive age between 1936 and 1940 gives a TF rate of 9.4, and for 1946 and 1950 the TF value was 8.1 (Eaton and Mayer 1953.227). It is then very reasonable to estimate nine as the overall TF value to associate with those who died between 1941 and 1950. Thus our Figure 23 is tied to some sort of reality, but there are caveats. Firstly, the sex ratio is assumed, for purposes of discussion, to be 1:1. The model populations from which this is derived actually have a slightly lower TF. Secondly, Moita sample size is not Mary Jackes, Christopher Meiklejohn really adequate. Other problems have been laid out. Moita requires further consideration and has not been finalized. With Arruda we have the issue of partial excavation; some of the skeletons were lost; most skeletons have been mixed since excavation. With Casa da Moura we have a burial cave with multiple mixed disarticulated skeletons, smashed bones and skulls, and thousands of loose teeth. Despite these problems we feel we have at least one meaningful result. Fertility appears to have already been increasing in the late Mesolithic and on into the Neolithic. Our results suggest an increase in fertility of around two children per woman within the Mesolithic. We do not know the exact fertility, though our figure suggests a shift from ca. five to ca. seven, but we have some idea of the differential. Our figure suggests a slightly larger increase between Arruda in the Mesolithic and Casa da Moura in the Neolithic, to a fertility perhaps approaching nine. Above all, we have a method by which we can test whether samples provide demographic parameters that are within or outside the bounds of biological reality. There is independent evidence of the change proposed for the Mesolithic/Neolithic transition in Portugal – a change that begins within the Mesolithic and continues on into the Neolithic, so that a continuous process rather than an “event” must be the interpretation. Dental attrition levels altered (Lubell et al. 1994), suggesting a switch to a progressively softer diet. Independent evidence, based on dental metrics, suggests that the weaning period was shortened (Jackes et al. 1997); along with softer food this would argue for reduced birth intervals. Arruda females were doing something different from Moita females, a shift towards a more sedentary pattern is suggested (Jackes, Lubell 1999), the evidence for this deriving from the internal geometry and density of femoral bone and external features of the femora and tibiae (with a control over the method being provided by Nordin’s Index derived from femoral x-rays and control for age by attention to dental wear, and especially by a study of femoral neck x rays). This again suggests circumstances under which more frequent pregnancies would be likely. There is also the suggestive fact that Arruda contains foetal and neonate individuals, based on long bones rather than mandibles. We believe that Arruda was probably a near permanent residential site. Bocquet-Appel’s newly proposed value: P = (5–19)/(Total 5 and over) is useful in avoiding some age assessment errors (Bocquet-Appel 2002). Logically, and in actual fact when tested on model data, it is perfectly correlated with mean childhood mortality (MCM) of Figure 22 (x-axis). It has a slightly non-linear relationship with J:A when tested on model data. Its valid upper value is likely to be 0.350 which is the equivalent of ca 0.380 J:A, predicting a TF of 12 children. We note that most of Bocquet-Appel’s sites fall below P = 0.350 and we would suggest this as a cut-off value, beyond which bias must be suspected. It is interesting that Bocquet-Appel’s high values all derive from samples of small size. Bocquet-Appel’s suggestion of a stationary population for the Mesolithic appears to us justified in general terms based on our work on sites in Scandinavia and in the Iron Gates region of the Danube valley (Meiklejohn et al. 1997; Jackes et al. in press). We believe we have provided evidence in support of Bocquet-Appel’s argument that there was an increase of fertility in the early Neolithic. However, we suggest that the possibility of a demographic trend through time from Moita to Arruda is noteworthy, since independent evidence suggests that the two sites have differences in terms of dental attrition, dental pathology, tooth size, stable isotope central values, cortical thickness and femoral morphology. We absolutely agree that a reduction in the birth interval is the mechanism of population increase, but we see that reduction beginning in the late Mesolithic with an increasingly sedentary life. The differences between the sites are specific and scaled to trends rather than absolutes. We see no evidence of genetic change accounting for the biological differences, no evidence of an incoming population (e.g. Jackes et al. 2001). Absent biological evidence for large-scale population movement, continuity of demographic trends is suggested, and no small group of precursor males would explain the apparent fertility increases in the development and transition from late Mesolithic to early Neolithic. We have focused here on samples that cover around 2000 years, from 8000 to 6000 calBP, a period of sea level change and some climatic variation. Fertility rates in Europe could have reached our theoretical maximum according to the data used by Bocquet-Appel from Wandersleben (Bach 1986) and Grossbrembach (Ullrich 1972), sites that have adequate samples and fall within the limits of biological reality. But these sites are from the very late LBK or the Bronze Age, and the Early Bronze Age of Central Europe provides sample sizes and age distributions of such diversity (Berner 1992) that the period of Building a method for the study of the Mesolithic-Neolithic transition in Portugal agricultural intensification appears to require a separate study focusing on the interpretation of the variations. Leaving aside questions surrounding such later sites, Casa da Moura provides the best data for the European Neolithic in which a considerable increase in fertility can be discerned: yet the site is not ideal because it covers a long period of time (our two dates alone cover 1000 years). We propose that studies of the transition be focused on sites within a restricted geographical area, in which the demographic and biological variations introduced by population movements can be excluded, where there is tight control over dating. The earliest Casa da Moura date calculated at 25% marine diet certainly overlaps the latest Mesolithic dates for central Portugal, so that we have some assurance that our data are directly relevant to the question of the demography of the transition. CONCLUSION In pointing out the problems associated with the sample of human skeletons from Arruda, we have done no more than is absolutely necessary. Without careful consideration of the nature of the samples, and the methods used to arrive at the estimates of sample size, and without methods of comparison among samples, discussion of Mesolithic/Neolithic transition demography is an unstable foundation. We propose that the methods used across sites of different types must take into account problems arising from burial practices, from excavation and curation and that such methods must be applied with the greatest of care. Far from destroying confidence in the sample, we have developed a method that suggests that our data have some biological validity. It is important that the Portuguese data be approached in such a cautious manner because it is so important in providing us with reasonably large samples across the Mesolithic/Neolithic transition. Although internally consistent, the evidence of a stationary Mesolithic population with low fertility, as previously presented (Meiklejohn et al. 1997; Jackes et al. in press), was based on Scandinavian samples that are not adequate and on Iron Gates material about which some questions remain. More detailed work on the Iron Gates sites presents the best hope of confirming our hypotheses. The situation in Portugal is simpler than in the Iron Gates region. There is no evidence for large popu lation replacement and if there were some trans- coastal arrivals these seem to have provided no detectable genetic input. It is unlikely that the biology of the indigenous population would have been altered (absent some unknown founder effect) without either population replacement or differential fertility. However, the increase in fertility appears to have been established prior to the Neolithic and increased fertility must be attributed to some alteration of life-way. A reduced birth interval, suggesting earlier weaning, is likely (Jackes et al. 1997). Increasing sedentism and changes in weaning food would be sufficient explanation for this. The evidence thus suggests that the base-camp type settlement at Arruda does indeed provide the appropriate scenario and it appears that Arruda may be more likely than Moita to provide the timing for this along the Muge valley. It is to be noted that a slow and steady increase, rather than a sudden leap in fertility, is proposed. It is also to be noted that total fertility (TF) levels of around eight can be considered as biologically feasible for the early Neolithic in Portugal. At the same time, it is essential to realize that this is a demographic parameter, not an actuality. It is inconceivable that every woman would have had eight children and that every one of those eight children would have grown to reproductive age and had eight children. Nevertheless, we have come closer to an understanding of the timing, mechanism and scale of population increase in central Portugal over the period 8000 to 6000 calBP. NOTE ADDED IN PROOF Material from the 1964 excavation has now been moved, and stored with earlier Arruda finds at the Serviços Geológicos in Lisbon. Prior to 2000, the material had undergone at least two periods of flooding (Rolao pers. comm.) and had become mixed. On the basis of a brief examination of the material by Jackes and Lubell in September 2004, we can confirm the number of mandibles seen by Lubell in 2002. However, when the material was moved to the new storage facility, a previously unrecorded skull and mandible were found. Furthermore, another very fragmentary mandible, with a label indicating that it was collected in 1965, has been located by José António Anacleto, Serviços Geológicos, to whom we are indebted for his assistance. Mary Jackes, Christopher Meiklejohn ACKNOWLEDGEMENTS We wish to acknowledge the work of Portuguese archaeologists during the 19th and early 20th centuries. They were truly ahead of their time. The problems of working with material excavated long ago are legion and it is necessary to document the problems so that we can judge the value of the collections. Material that has been lodged in museums for over 120 years has naturally been subject to the vicissitudes of time. That so much has been preserved and the records are as good as they are demonstrates the sophistication of these early archaeologists. The work reported here has been done with the assistance of many people over a period that now approaches 35 years. Meiklejohn’s work in 1969 was supported by a Canada Council Doctoral Fellowship. The work of Jackes and Meiklejohn in the 1980s was supported by two Social Sciences and Humanities Research Council of Canada grants (410–84–0030 and 410–86–2017) in collaboration with David Lubell. Over the years a number of students have assisted us in data collection. Meiklejohn would especially like to thank Catherine T. Schentag, while Jackes would like to acknowledge Pamela Mayne-Correia, Cidália Duarte and Gary Tait for work on Casa da Moura loose teeth. Deborah Ross worked on the methodology of tooth identification. Dr. Gerd Weih’s professionalism and general assistance with our Moita and Arruda analyses were invaluable. A number of colleagues in Portugal contributed significantly to our research. Dr. M. M. Ramalho, now Vice- Presidente Área Técnico-Científica Instituto Geológico e Mineiro Portugal, authorized access to Muge materials in the 1980s. Dr. J. Brandao, Conservador do Museu Geológico, Lisboa, permitted Lubell access to the 1964 Arruda skeletons and delegated his colleague José António Anacleto who provided invaluable assistance. A. H. Bacelar Gonçalves, Museu de História Natural Faculdade de Ciencias da Universidade do Porto, provided essential help in Porto. Dr. J. Rolao, Universidade Autonoma de Lisboa, through his archival work and his current investigations at Muge, greatly faciliated our work. The X-rays were taken at the Janares Clinic, Sintra, by Paulo de Deus Machado. Jackes thanks Dr. Mihael Budja for his invitation to participate in the Ljubljana conference, and the University of Alberta for a travel grant that covered air fare. 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