4 CONCLUSIONS The most imporant factors that reduce the productivity of the watermelon are high temperatures, high humidity, exces rain, pests and diseases. The results obtained in our study indicate that the use of different mulch system is a potential factor in aphids control on watermelons. during the first assessment, the aphid population density was equally as the recorded maximum. That indicates the observations of the visual traps are necessary, anon after the planting, in order to create the aphids control management strategy. The aphids' flight maximum in watermelon growth at Pula in 2008 was occurred two weeks earlier than at Opuzen (2004, 2005). Ten days after the planting, The effect of different mulches on aphid populations in vegetable crops has to be continued. 5 ACKNOWLEDGEMENT The authors thank head of Valtura jail who gave up part prisoners who were of valuable assistance during aphid of jail farm for carrying out this experiment and sampling. 6 LITERATURE Brown, J.E., Dangle, J.M., Woods, F.M., Tilt, K.M., Henshaw, M.D., Griffey, W.A., West, M.S. 1993. Delay in mosaic virus onset and aphid vector reduction in summer squash grown on reflective mulches. HortSci., 28, 9: 895-896. Csizinszky, A.A., Schuster, D.J., Kring. J.B. 1995. Color mulches influence yield and insect pest populations in tomatoes. J. Amer. Soc. Hort. Sci., 120, 5: 778-784. Farias-Larios, J., Orozco-Santos, M. 1997a. Color polyethylene mulches increase fruit quality and yield in watermelon and reduce insect pest populations in dry tropics. Gartenbauwissenschaft, 62, 6: 255-260. Farias-Larios, J., Orozco-Santos, M. 1997b. Effect of polyethylene mulch colour on aphid populations, soil temperature, fruit quality, and yield of watermelon under tropical conditions. New Zeal. J. Crop Hort., 25, 369374. Goreta, S., Perica, S., Dumičić, G., Bučan, L., Zanić, K. 2005. Growth and yield of watermelon on polyethylene mulch with different spacings and nitrogen rates. HortSci., 40, 2: 366-369. Johnson, J.M., Hough-Goldstein, J.A., Vangesell, M.J. 2004. Effects of straw mulch on pest insects, predators, and weeds in watermelons and potatoes. Environ. Ent., 33, 6: 1632-1643. Kring, J.B., Schuster, D.J. 1992. Management of insects on pepper and tomato with UV-reflective mulches. Fla. Entomol., 75, 1: 119-129. Lamont, Jr.W.J. 1993. Plastic mulches for the production of vegetable crops. HortTehnology, 3, 1: 35-39. Millar, I.M. 1994. A Catalogue of the Aphids (Homoptera: Aphidoidea) of Sub-Saharan Africa. Agricultural Research Council, Pretoria. Saucke, H., Döring, T.F. 2004. Potato virus Y reduction by straw mulch in organic potatoes. Ann. Appl. Biol., 144, 3: 347-355. Soltani, N., Anderson, J.L., Hamson, A.R. 1995. Growth analysis of watermelon plants grown with mulches and rowcovers. J. Amer. Soc. Hort. Sci., 120:1001-1009. Stapleton, J.J., Summers, C.G. 2002. Reflective mulches for management of aphids and aphid-borne virus diseases in late-season cantaloupe (Cucumis melo L. var. cantalupensis). Crop Prot., 21, 891-898. Summers, C.G., Mitchell, J.P., Stapleton, J.J. 2005. Mulches reduce aphid-borne viruses and whitefies in cantaloupe. Calif. Agr., 59, 2: 90-94. Trdan, S., Znidarčič, D., Kač, M., Vidrih, M. 2008. Yield of early white cabbage grown under mulch and non-mulch conditions with low populations of onion thrips (Thrips tabaci Lindeman). Int. J. Pest Manag., 54, 4: 309-318. Walters, S.A. 2003. Suppression of watermelon mosaic virus in summer squash with plastic mulches and row covers. HortTehnology, 13, 2: 352-357. White, J.M. 2003. Watermelon yield and size when grown on four mulch colors. Proc. Fla. State Hort. Soc., 116: 138139. White, J.M. 2004. Summer squash yield and fruit size when grown on eight mulch colors in central Florida. Proc. Fla. State Hort. Soc., 117: 56-58. Agrovoc descriptors: marshes; swamps; wetlands; fertilizer application; fertilizers; botanical composition; flora; mowing; harvesting; biomass Agris category code: F01; F70; F04 Changes in floristic composition over three years of Ljubljana marsh grassland in relation to cutting and fertilising management Matej VIDRIH1, Jure ČOP2, Stanislav TRDAN3, Klemen ELER4 Received August 6, 2008, accepted July 8, 2009. Delo je prispelo 6. avgusta 2008; sprejeto 8. julija 2009. ABSTRACT A research in Ljubljana marsh was conducted from 2004 to 2006 with the aim to determine how the regime of cutting and fertiliser application over several years influences on the floristic composition of meadow sward. Field sampling plots in split-plot design with four replications were set up on two different types of grassland, one belonging to Arrhenatherion (sampling plot T1), the other to Molinion alliances (sampling plot T2) in 1999. The main plots represented the frequency of 4 cutting regimes (2 cuts with normal and delayed first one, 3 and 4 cuts per year) and sub-plots represented the fertiliser regime (no fertiliser, PK and NPK fertiliser with two different amounts of N). After five years, the cutting, and especially fertiliser application, significantly altered the floristic composition. In floristic composition of Arrhenatherion plot more frequent cutting in combination with higher amount of N fertilisation increased the proportion of grasses (92.7 % on a fresh matter basis). This was mostly observed in 2004. Legumes proportion (15.4 %) increased mainly on plots where PK fertiliser was used and a first cut was retarded. When N fertiliser was used on Molinion plot in all treatments with cutting herbs (forbs) increased their proportion up to 65 % in average. The proportion of legumes in sward of this plot was neglecteable that's way treatments did not have any special effect on them. Key words: Ljubljana marsh, grassland, cutting, fertilising, floristic composition, biomass IZVLEČEK SPREMEMBE V FLORISTIČNI SESTAVI RUŠE LJUBLJANSKEGA BARJA SKOZI TRI LETA V ODVISNOSTI OD ČASA KOŠNJE IN INTENZIVNOSTI GNOJENJA Na Ljubljanskem barju smo v obdobju 2004-2006 opravili raziskavo, s katero smo želeli ugotoviti, kako vplivata število košenj in gnojenje skozi daljše obdobje na floristično sestavo ruše. Travniška poskusa v split-plot zasnovi s štirimi ponovitvami sta bila zasnovana na dveh tipih poskusnih ploskev, ki pripadata zvezama Arrhenatherion in Molinion v letu 1999. Glavne parcele so predstavljale štiri režime pogostnosti košnje (2-kosna raba z zapoznelo in standardno prvo košnjo, 3-kosna in 4-kosna raba), podparcele pa način gnojenja (negnojeno, gnojeno z gnojili PK in NPK, gnojeno z dvema različnima odmerkoma N). Po petih letih sta košnja in predvsem gnojenje zelo spremenili videz travišča in vplivala na floristične karakteristike travne ruše. V travni ruši poskusnih ploskev zveze Arrhenatherion je pogostejša košnja v kombinaciji z večjim odmerkom dušika vplivala na večji delež trav (92,7 % v svežem zelinju). Ta sprememba je bila najbolj izražena v letu 2004. Delež mase metuljnic (15,4 %) se je najbolj povečal po gnojenju s PK in zapoznelo prvo košnjo. Na poskusni parceli zveze Molinion se je najbolj povečal delež zeli (največ 65 %), in sicer v vseh postopkih košnje ter tedaj, kadar je bil uporabljen dušik. Delež metuljnic v tej ruši je bil zanemarljiv. Ključne besede: Ljubljansko barje, travinje, košnja, gnojenje, floristična sestava, biomasa 1 Teach. Assist., Ph. D., Jamnikarjeva 101, SI-1111 Ljubljana, e-mail: matej.vidrih@bf.uni-lj.si 2 Assist. Prof., Ph. D., ibid. 3 Assist. Prof., Ph. D., ibid. 4 Teach. Assist., Ph. D., ibid. 1 INTRODUCTION The Slovene grasslands, predominantly existing as semi-natural vegetation, cover 60 % of agricultural land (5000 km2) (Čop, 2006). In addition 2700 km2 of abandoned grasslands have reached different phases of plant succession toward forest climax vegetation for the last fifty years. On the managed grasslands the cutting system prevails with two to three cuts on mesotrophic, and one cut on oligotrophic karst and wet grasslands. Intensive cutting and grazing systems were also introduced on many farms during last few decades. Due to this development and abandoning of herbage production in marginal grassland areas many species-rich meadows and mountain pastures are endangered (Čop et al., 2004). In temperate climate zone grassland management is a key factor which determines sward floristic composition (Hopkins and Holz, 2005). Generally, intensification of herbage production reduces sward plant diversity while improving its agronomic value (Plantureux et al., 2005). Grasslands in marginal areas can be exception to this and preservation of the remaining species-rich grassland is a primary goal of nature conservation (Armbruster and Elsäßer, 1997; Mountford et al, 1993) The continuation of traditional ways of grassland management that would best preserve biodiversity is often not compatible with the requirements of intensive livestock production (Isselstein et al., 2005; Critchley et al., 2002; Hopkins et al., 1990). Therefore, we have been performing a long term field study to investigate effects of cutting regime and fertiliser inputs on sward floristic composition of two grassland types located in an environmentally sensitive area such as Ljubljana marsh area (Seliškar, 2000; Jogan et al., 2004; Hacin et al, 2001). Almost all grasslands represent unimproved and semi-improved hay meadows traditionally mowed twice a year. In the past there was also a combination of lax spring and autumn grazing along with summer cutting (Verbič, 2000). Undesired plant succession has occurred on many parts of Ljubljana marsh, area that can be assigned as typical environmentally sensitive one. Of its 160 km2 surface app. 120 km2 are covered by semi-natural grassland, which is highly diverse, often supporting considerable floristic diversity at a local scale, providing habitats for invertebrate and other animal groups and delivering a range of ecosystems and socio-economic functions. On this Ljubljana marsh Arrhenatherion alliance is a dominant vegetation. Much less grassland area belongs to Molinion, Filipendulion, Magnocaricion, Caricion davallianae and Phragmition communis alliances (Seliškar, 1986). Aiming for sustainable grassland production in this area, a research was conducted to test the effects of cutting and fertiliser treatments on herbage production and floristic composition of Arrhenatheretum elatius and Molinia caerulea grasslands. We wanted to test the influence of two typical grassland management measures on floristic changes in meadow sward. 2 MATERIAL IN METHODS In March 1999, two sampling plots were established on semi-natural grassland of Ljubljana marsh (lat. 45°58' N, long. 14°28' E, alt. 295 m). One plot was on grassland with predominant Arrhenatherum elatius (T1) and the other on fen meadow with predominant Molinia caerulea (T2). Both plots were arranged in split-plot design with four replications. Three cutting regimes were allocated as the main plots and four fertiliser treatments as sub-plots. Cutting regimes for T1 were: 2 cuts with delayed first one, 3 cuts and 4 cuts per year, and for T2 were: 2 cuts with a 'normal' and delayed first one and 3 cuts per year. Fertiliser treatments were 0 NPK (= no), 35 kg P + 133 kg K ha'yr1 (= PK), 50 kg N ha'cut"1 applied to the first cut only + 35 kg P + 133 kg K ha-1 yr-1 (= N(1)PK) and 50 kg N ha-1 cut-1 applied to each of 2 or 3 or 4 cuts + 35 kg P + 133 kg K ha-1 yr-1 (=N(c)PK). The sub-plot size was 2.5 x 4 m in T1, and 2 x 4 m in T2 (Čop et al., 2001). In the fourth trial year (2002), the soil was moderately acid (pH/CaCl2 = 4.9 -5.2) with low to moderate P and moderate to high K content (P = 1.9 - 5.5 mg, K = 10.6 - 29.5 mg) in T2. Fertilising with PK in previous years had a positive effect on soil nutrient status only on Molinia caerulea fen meadow (T2 sampling plot). The chemical properties of soil on Arrhenatherum elatius grassland (T1 sampling plot) in four most intensive treatments in the eight year are shown in Table 1. Table 1: Chemical properties of soil on four plots of sampling plot T1 in spring 2006 after 7 years of experiment (phosphorus and potash were determined by extraction in ammonium lactate). Treatment pH/CaCl2 P205/(mg/100 g) K20/(mg/100 g) no 7.0 3.5 1 1 .8 four cut PK 6.9 14.9 21.1 regime N^PK 7.0 1 0.9 1 6.2 N(c)PK 6.9 15.6 16.4 Results presented here are derived from the first cut of the sixth, seventh and eight trial years and comprise proportions of floristic groups (grasses belonging to botanical family Poaceae, legumes belonging to botanical family Fabaceae and herbs belonging to remaining botanical families) in herbage. The analyses were performed by means of hand separation of fresh herbage samples into plant floristic groups which were afterwards weighted. The size of sampling area was 0.5 X 0.8 m. According to Braun-Blanquet method (1964) a floristical survey on species presence (a combine assesment of cover and abundance) was also conducted. Statistical analyses of data were done by ANOVA and only p values (significance level) are shown for both factors and their interaction. Data in proportions were transformed using an equation Y = 2*arcsine(sqrt(x)). Results for ANOVA are presented only for the eight (2006) trial year. 3 RESULTS In 2006, we made a floristical survey at all 96 sub-plots (3 cutting regimes, 4 fertiliser treatments regimes, 4 replications) of sampling plots T1 and T2. The community of Arrhenatherum elatius grassland consisted in total of 89 species (Table 3), from most abundant to rare ones. The most frequent grasses were Arrhenatherum elatius, Festuca rubra and Dactylis glomerata. The group of legumes was represented by Vicia cracca, Lathyrus pratensis and Trifolium pratense whereas Equisetum palustre, Galium mollugo and Ranunculus repens prevailed in a group of herbs. The community of Molinia caerulea consisted in total of 85 species (Table 4). Three most frequent grasses were Molinia caerulea, Anthoxanthum odoratum and Holcus lanatus. From a group of legumes the most frequent were Vicia cracca, Lotus uliginosus and Lotus corniculatus, meanwhile the group of herbs was represented the most frequently by Filipendula ulmaria, Galium mollugo and Potentilla erecta. In sampling plot T1 a higher management intensity (special with four cuts per year and applying 50 kg of N at each cut) encouraged the group of grasses (Figure 1; bigger circles in ternary graphs), which is most noticeabled in year 2005. The highest fresh herbage proportion of grasses (92.7 %) was measured on plot with treatment of three cuts and N50PK fertiliser in 2005. The highest proportion of legumes (15.4 %) was determined on plot with treatment of two cuts with delayed first cut and PK fertiliser in 2006 and the highest proportion of herbs (49.4 %) was measured on plot with treatment of two cuts with delayed first cut and no fertiliser in 2004. Cutting regimes had significant effect on proportion of grasses and legumes, fertiliser treatments had only on legumes and none of them on herbs proportion (Table 2). Due to the lack of legumes (important ones) in the sward none of the treatments came near to the recommended proportion of floristic groups (Fig. 1: triangle symbol in ternary graphs) in sward of semi natural grassland when looking from the forage production view (Dietl, 1982). Grasses Grasses Grasses Figure 1: Influence of cutting and fertiliser application on floristic composition of Arrhenatherion sampling plot (T1) in 2004 (a), 2005 (b) and 2006 (c) (o-most extensive management, O-most intensive management) (4 cuts) and position of optimal proportion (A) offloristic groups in semi natural grassland. In sampling plot T2, a lack of legumes in sward was even more expressed. However sward on plots which received only PK fertiliser showed the increase in proportion of legumes and this fact led to the highest proportion of them (11.4 %) on plot with treatment of two cuts and PK fertiliser in 2005. On the other hand, with increasing intensity of management (specially with N fertiliser) of herbs (Figure 2; year 2005) which resulted in their highest proportion of herbs (85.0 %) on reached the highest proportion (88.7 %) on plot with plot with treatment of two cuts and delayed first cut and treatment of two cuts and no fertiliser in 2005. applying 50 kg of N at each cut in 2004. Grasses Figure 2: Influence of cutting and fertiliser application on floristic composition of Molinion sampling plot (T2) in 2004 (a), 2005 (b) and 2006 (c) (o-most extensive management, O-most intensive management) (4 cuts) and position of optimal proportion (A) offloristic groups in semi natural grassland. The proportion of floristic groups on sampling plot T2, between cutting regime and fertiliser treatments had no measured in eight year, first cut, was affected less by significant effect on none of the floristic groups. cutting than fertiliser application (Table 2). Interaction Table 2: Significance level (alpha risk) for the test of effects of the cutting regime (C) and fertiliser treatments (F) on floristic groups in herbage of the 1st cut in Arrhenatherion (T1) and Molinion (T2) sampling plots, 8th trial year. Sampling plot T1 Grasses Legumes Herbs Sampling plot T2 Grasses Legumes Herbs Cutting regime (C) Fertiliser treatments (F) C x F 0.002 0.033 0.455 0.127 <0.001 0.117 0.072 0.468 0.080 0.041 0.454 0.064 0.001 <0.001 0.001 0.920 0.100 0.915 Table 3: Floristical survey of the Arrhenatherum elatius grassland after Braun-Blanquet method (sampling plot T1) according to cutting regime and fertilising (8th trial year) *. 2 cuts (delayed) 3 cuts 4 cuts no PK N,PK NPK no PK N,PK NPK no PK N,PK NPK Anthoxanthum odoratum 1 + + + + Arrhenatherum elatius 2 2 2 3 + 3 3 3 + 2 1 3 Dactylis glomerata 1 + + 1 + 1 1 1 + 1 1 1 Festuca pratensis + + 1 1 1 1 1 1 + Festuca rubra agg. 2 1 1 + 2 1 1 3 1 3 + Helictotrichon pubescens + + 2 1 2 2 1 1 1 1 Holcus lanatus + 1 1 + + Poa trivialis + + + 1 Lathyrus pratensis + 1 + + + + Medicago lupulina + + + Trifolium pratense + 1 1 1 + Vicia cracca + + + + + + + + + + Achillea millefolium + + + + 1 + 2 1 + + + 1 Ajuga reptans + + + Angelica sylvestris + + + + Calystegia sepium + + + + Campanula patula + + + + + + Centaurea jacea + + + + + + + 1 + + 1 + Cerastium holosteoides + + + Cirsium oleraceum + + + + Convolvulus arvensis + + + + + + + + Cruciata glabra + + + + + Daucus carota + + + + + Equisetum palustre 3 1 1 + 3 1 + + 3 + + + Erigeron annuus + + + Galium mollugo 1 2 2 2 1 2 1 2 1 + + 1 Glechoma hederacea + + + Leontodon hispidus + + + + + Leucanthemum ircutianum + + + + + + + + + 1 2 + Lythrum salicaria + + + + + Silene latifolia + + + + + + Mentha aquatica + + + + + Mentha longifolia + + + Pastinaca sativa + + + + + + + Pimpinella major + + + + + Plantago lanceolata + 1 + 1 1 1 + + + Ranunculus acris + + 1 1 1 + + + + Ranunculus repens + + + + + + + + + + + + Rumex acetosa + + + + Taraxacum officinale + + + + Verbascum sp. + + + + Veronica persica + + + + + + + Total number of species 29 27 22 29 24 26 30 30 28 25 24 28 * Species with cover < 1 %, appeared in one or two treatments only, are not included in the table. Table 4: Floristical survey of the Molinia caerulea fen meadow after Braun-Blanquet method (sampling plot T2) according to cutting regime and fertilising (8th trial year) *. 2 cuts (delayed) 2 cuts 3 cuts no PK N,PK NPK no PK NPK NPK no PK N,PK NPK Anthoxanthum odoratum 1 1 + + 1 + + 1 1 1 1 1 Arrhenatherum elatius 1 + + Brachypodium pinnatum 2 2 + + 2 2 2 + + 1 Briza media + + + + + + + + + Carex flava + + + + + + + Dactylis glomerata + + 1 1 Deschampsia cespitosa + 1 Festuca ovina agg. + 1 Festuca pratensis + + + + + 2 + + Festuca rubra agg. 1 + 1 1 1 1 1 2 2 2 Helictotrichon pubescens + + 2 + + Holcus lanatus + 1 2 1 + 1 1 + 1 Luzula campestris + + + + + + Molinia caerulea 4 + + + 4 + 3 + + + Lotus corniculatus + 1 + Lotus uliginosus + 1 + + 1 + 1 + Vicia cracca 1 + 1 + + + + + + + Angelica sylvestris + + + + + + + + + + + Betonica officinalis + + + + + + + 1 + + + Centaurea jacea + + + + + + Cirsium oleraceum + + + Cruciata glabra + + + + + Daucus carota + Equisetum palustre 1 + Filipendula ulmaria 2 1 1 2 1 1 2 1 1 + 1 1 Galium mollugo + 1 1 2 2 2 1 3 1 1 2 2 Leucanthemum ircutianum + + + + + 1 1 1 Lysimachia vulgaris + Lythrum salicaria + + + + + + + + Plantago lanceolata + + + + + + + + + + Potentilla erecta 1 + + + + + + + 1 + + + Ranunculus acris + + + + + Ranunculus repens + + + + + Rumex acetosa + + + + + + Thymus alpestris + + + + + + Total number of species 21 26 20 18 18 22 19 15 26 36 28 25 * Species with cover < 1%, appeared in one or two treatments only, are not included in the table. 4 DISCUSSION In this study, we explored the possibility of combining agricultural and nature conservation objectives in threatened wet grasslands at the Ljubljana marsh by applying different cutting regimes and fertiliser amounts. Both factors were tested at low (no for fertiliser) to moderate levels to confirm their effects on the floristic composition of two seminatural unimproved meadows. After eight trial years the data show that the NPK and PK fertilising treatments improved floristic composition on T1 sampling plot regarding fodder quality, while on T2 sampling plot these two treatments increased the proportion of herbs, which are not the most appropriate for the nutrient poor grassland community as the one belonging to Molinion alliance. Grasses, especially the competitive Arrhenatherum elatius, Dactylis glomerata and Festuca pratensis, responded to moderate increasing number of cuts and fertiliser input with an increase in their proportion in the sward of sampling plot T1. This response is considered as typical and is described elsewhere (e.g. Tallowin, 1996; Wyss, 2002). Under similar treatment conditions, the stress tolerant Molinia caerulea, which initially prevailed in the sward of sampling plot T2, was replaced mainly by forbs (Filipendula ulmaria within delayed 2 cuts and Plantago lanceolata and Galium mollugo within other two cutting regimes) (Čop et al, 2004). Every fertiliser input increased proportion of tall grasses belonging to Arrenatherum elatius grassland and tall forbs belonging to Molinia caerulea fen meadow as was also got in the literature (Smith et al., 1996). But we should also not forget that amount of precipitation and solar radiation can have significant effect on grassland production. And also floristic composition of sward is only one variable in multi dimensional space in which other two important variables are dry matter yield (quantity) and nutrients yield (quality). 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