{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-2K0PQ0AZ/c24898fa-9a07-49b7-9c09-8cd8abb101c0/PDF","dcterms:extent":"275 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-2K0PQ0AZ/c23df949-d2c0-41a5-885f-f897c76c5a87/TEXT","dcterms:extent":"24 KB"}],"edm:TimeSpan":{"@rdf:about":"1998-2025","edm:begin":{"@xml:lang":"en","#text":"1998"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-2K0PQ0AZ","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-KC6O72BG"},{"@xml:lang":"sl","#text":"Acta chimica slovenica"}],"dcterms:issued":"2016","dc:creator":["Bratkovič, Tomaž","Molek, Peter"],"dc:format":[{"@xml:lang":"sl","#text":"številka:4"},{"@xml:lang":"sl","#text":"letnik:63"},{"@xml:lang":"sl","#text":"str. 914-919"}],"dc:identifier":["ISSN:1318-0207","COBISSID:4216689","URN:URN:NBN:SI:doc-2K0PQ0AZ"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"dc:subject":[{"@xml:lang":"en","#text":"antibody"},{"@xml:lang":"en","#text":"epitope mapping"},{"@xml:lang":"en","#text":"mimotope"},{"@xml:lang":"en","#text":"peptide"},{"@xml:lang":"en","#text":"phage display"}],"dcterms:temporal":{"@rdf:resource":"1998-2025"},"dc:title":{"@xml:lang":"sl","#text":"Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries|"},"dc:description":[{"@xml:lang":"sl","#text":"Detailed knowledge of antigenic determinants is crucial when characterizing therapeutic and diagnostic antibodies, assessing vaccine effectiveness and developing epitope-based vaccines. Most epitope mapping approaches are labor intensive and costly. In this study, we evaluated panning of phage-displayed random peptide libraries against antibodies as a tool for cognate epitope identification. We used six antibodies directed to three model protein antigens as targets to show that the approach is applicable to both mono- and polyclonal antibodies. The technique is well-suited especially for identification of linear epitopes. Mapping of conformational epitopes is more challenging, tends to be more subjective and requires use of computational tools. Nevertheless, when combined with functional data such as structure-activity relationship of antigen muteins, one can make reliable conformational epitope predictions based on phage display experiment data. As the described approach is fast and relatively inexpensive, we suggest it is employed early in antibody characterization and later validated by complementary methods"},{"@xml:lang":"sl","#text":"Poznavanje antigenskih determinant je ključnega pomena v zgodnjem razvoju terapevtskih in diagnostičnih protiteles, pri vrednotenju učinkovitosti klasičnih ali razvoju epitopnih cepiv. Večina metod za kartiranje epitopov protiteles je dragih in eksperimentalno zahtevnih. V pričujoči raziskavi smo ocenjevali primernost afinitetnega presejanja knjižnic naključnih peptidov, predstavljenih na bakteriofagih, kot orodja za identifikacijo epitopov. Kot tarče v afinitetnih selekcijah smo uporabili šest različnih mono- in poliklonskih protiteles proti trem modelnim proteinom. Ugotovili smo, da pristop omogoča jasno identifikacijo linearnih epitopov, medtem ko je kartiranje konformacijskih epitopov bolj zahtevno, saj je manj zanesljivo in zahteva uporabo računalniških orodij. Kljub temu je s podrobnim poznavanjem antigena (npr. z analizo vpliva mutacij na aktivnost proteinskega antigena) na osnovi peptidnih mimetikov tudi konformacijske epitope mogoče napovedati s precejšnjo zanesljivostjo. Ker opisan pristop omogoča hitro in poceni razkritje epitopov, predlagamo njegovo uporabo v zgodnjih fazah razvoja protiteles s kasnejšo validacijo antigenskih determinant s komplementarnimi metodami"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-2K0PQ0AZ","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-2K0PQ0AZ"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-2K0PQ0AZ/c24898fa-9a07-49b7-9c09-8cd8abb101c0/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-2K0PQ0AZ/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-2K0PQ0AZ"}}}}