{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-G2LEQPEJ/c0cf2e17-43cf-4ce9-aca6-256bea426bf9/HTML","dcterms:extent":"45 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-G2LEQPEJ/4dadb215-a900-479e-9550-76575e90fbfc/PDF","dcterms:extent":"966 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-G2LEQPEJ/12fa7293-3714-4fee-b895-00d4ac739bb0/TEXT","dcterms:extent":"38 KB"}],"edm:TimeSpan":{"@rdf:about":"2004-2025","edm:begin":{"@xml:lang":"en","#text":"2004"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-G2LEQPEJ","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/urn:nbn:si:spr-1fyqipbt"},{"@xml:lang":"sl","#text":"Acta agriculturae Slovenica"}],"dcterms:issued":"2012","dc:creator":["Bolha, Luka","Dušanić, Daliborka","Narat, Mojca","Oven, Irena"],"dc:format":[{"@xml:lang":"sl","#text":"številka:1"},{"@xml:lang":"sl","#text":"letnik:100"},{"@xml:lang":"sl","#text":"str. 19-28"}],"dc:identifier":["ISSN:1581-9175","ISSN:1854-1941","COBISSID:3081864","URN:URN:NBN:SI:doc-G2LEQPEJ"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Biotehniška fakulteta"},"dc:subject":[{"@xml:lang":"en","#text":"gene expression"},{"@xml:lang":"sl","#text":"genetsko izražanje"},{"@xml:lang":"sl","#text":"kvantitativni PCR v realnem času"},{"@xml:lang":"en","#text":"molecular genetics"},{"@xml:lang":"sl","#text":"molekularna genetika"},{"@xml:lang":"en","#text":"RT-qPCR"},{"@xml:lang":"en","#text":"tissue cells"},{"@xml:lang":"sl","#text":"tkivne celice"},{"@rdf:resource":"http://www.wikidata.org/entity/Q856198"}],"dcterms:temporal":{"@rdf:resource":"2004-2025"},"dc:title":{"@xml:lang":"sl","#text":"Setting up a gene expression study for tissue cells by method of quantitative real-time PCR|"},"dc:description":[{"@xml:lang":"sl","#text":"Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings"},{"@xml:lang":"sl","#text":"Analiza izražanja genov z metodo reverznega pomnoževanja in kvantitativne verižne reakcije s polimerazo v realnem času (RT-qPCR) omogoča natančno in občutljivo določanje nivoja izražanja tarčnih genov. Vendar je za zagotavljanje točnosti, natančne interpretacije in ponovljivosti eksperimenta, predvsem pa relevantnosti rezultatov, pomembno natančno poznavanje korakov postopka. V tem prispevku opisujemo enostaven poskus, v katerem smo v celični liniji kokošjih hondrocitov, ki smo jih tretirali s spojino 5-fluorouracil, ki sproži apoptozo, določili relativno izražanje gena, ki kodira encim kaspazo 8.Ta primer smo uporabili za izpostavitev nekaterih pomembnih smernic pri oblikovanju študije izražanja genov v tkivnih celicah, analizi in interpretaciji rezultatov ter oblikovanju zaključkov"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-G2LEQPEJ","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-G2LEQPEJ"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-G2LEQPEJ/4dadb215-a900-479e-9550-76575e90fbfc/PDF"},"edm:rights":{"@rdf:resource":"http://rightsstatements.org/vocab/InC/1.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Univerza v Ljubljani, Biotehniška fakulteta"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-G2LEQPEJ/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-G2LEQPEJ"}}}}