3040 KB22 KB200220262002Gaastra, WimStarčič Erjavec, MarjancaŽgur-Bertok, Darjaštevilka:1letnik:45str. 9-15ISSN:1408-3671COBISSID:16159193URN:URN:NBN:SI:doc-HVKPWJXOenDruštvo biologov SlovenijeActa biologica slovenicabakterijeBase SequenceBaze, zaporedjeEscherichia ColiF FactorF faktorGeneticsgenimolekularna genetikaPlasmidsPlazmidiPolimerazna, verižna reakcijaPolymerase Chain ReactionTra region of the natural conjugative Escherichia coli plasmid pRK100 is F-like| Regija tra naravnega konjugativnega plazmida pRK100 bakterije Escherichia coli je podobna plazmidu F|The aim of the presented study was to identify the similarity of the pRK100 tra region with tra regions of other conjugative plasmids of Enterobacteriaceae. For this purpose several tra genes were amplified wikh PCRand the nucleotide sequences of the obtained PCR products were determined. The pRK100 is nucleotide sequences were compared to the nucleotide sequences deposited in GenBank and the nucleotide divergence between them was calculated. The obtained results clearly demonstrated, that the tra region of pRK100 is the most similar to the tra region of plasmid FCilj raziskave je bil ugotoviti podobnost regije tra plazmida pRK100 z regijami tra drugih konjugativnih plazmidov enterobakterij. V ta namen smo večgenov regije tra plazmida pRK100 pomnožili v PCR in določili nukleotidno zaporedje dobljenih produktov PCR. Nukleotidna zaporedja plazmida pRK100 smo primerjali z drugimi, deponiranimi, nukleotidnimi zaporedji in izračunali nukleotidno divergenco. Dobljeni rezultati so jasno pokazali, da je regija traplazmida pRK100 najbolj podobna regiji tra plazmida FTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo biologov Slovenije