392 KB24 KB200820252012Lipnik-Štangelj, MetodaŠarc, Lucijaštevilka:2letnik:5str. 65-72ISSN:1855-5640COBISSID:512240440URN:URN:NBN:SI:doc-JBCXBCS3enMedicinska fakultetaActa medico-biotechnicaacetaldehidacetaldehydeastrocitiastrocytesetanolethanolhormesishormezaHormeza - primer odgovora astrocitov po izpostavljenosti etanolu| Hormesis - an example of astrocyte response after ethanol exposure|Purpose: In the present work we describe hormesis and review documented hormetic responses in astroglial cells after exposure to different stressors. We present an example of the astrocyte response to acute and chronic ethanol exposure with hormetic characteristics. Methods: As an experimental model, newborn rat cortical astrocytes in culture were used. The cells were exposed to ethanol or the primary metabolite of ethanol (acetaldehyde) for 24 h or 7 days. After treatment, the protein content was determined, and the IL-6 levelsin the culture medium were measured using an enzymelinked immunoassay. Results: Treatment of astroglial cells with ethanol or acetaldehyde led to enhanced protein content and stimulated IL-6 production in the low concentration zone followed by diminished protein content and an inhibition ofIL-6 production at higher concentrations. Chronic exposure of astrocytes wasmore toxic than acute exposure for both compounds, and acetaldehyde was more toxic compared to ethanol. Conclusion: Ethanol and acetaldehyde representstressors for cultured astrocytes and evoke a typical hormetic response after acute and chronic exposureTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaUniverza v Mariboru, Medicinska fakulteta