{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-XZCYBMK3/b233fb5d-9111-49ea-9875-15032df72f7f/PDF","dcterms:extent":"211 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-XZCYBMK3/0e506c2f-da83-46be-a4e9-7ca974eab519/TEXT","dcterms:extent":"20 KB"}],"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-XZCYBMK3","dcterms:issued":"2008","dc:creator":["Dovč, Peter","Kunej, Tanja","Ogorevc, Jernej"],"dc:format":{"@xml:lang":"sl","#text":"Str. 85-91"},"dc:identifier":["ISSN:1581-9175","ISSN:1854-4800","COBISSID_HOST:2363272","URN:URN:NBN:SI:doc-XZCYBMK3"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Biotehniška fakulteta"},"dc:source":{"@xml:lang":"sl","#text":"Acta agriculturae slovenica. Suplement"},"dc:subject":[{"@xml:lang":"en","#text":"candidate genes"},{"@xml:lang":"en","#text":"cattle"},{"@xml:lang":"sl","#text":"govedo"},{"@xml:lang":"sl","#text":"kandidatni geni"},{"@xml:lang":"sl","#text":"kvantitativni lokusi"},{"@xml:lang":"en","#text":"mastitis"},{"@xml:lang":"en","#text":"molecular genetics"},{"@xml:lang":"sl","#text":"molekularna genetika"},{"@xml:lang":"en","#text":"QTL"},{"@xml:lang":"en","#text":"quantitative trait loci"}],"dc:title":{"@xml:lang":"sl","#text":"An integrated map of cattle candidate genes for mastitis| a step foward to new genetic markers| Integrirana karta kandidatnih genov za mastitis pri govedu| korak naprej pri razvoju novih genetskih označevalcev|"},"dc:description":[{"@xml:lang":"sl","#text":"To facilitate the development of new genetic markers for mastitis resistance or susceptibility we used genome-wide comparative approach to review all known mastitis-associated loci. We assembled into a map 233 loci that were identified by six different studz approaches (QTLs, association studies, expression experiments, AFLP-, miRNA- and epigenetic- studies). To integrate data from different sources and to identify overlapping regions we presented the results in the form of genetic map. The collected data represent genetic background for mastitis-related traits in cattle. Thirty most promising candidate genes (associated with mastitis in different study approaches or at least in two independent studies, or/and overlapping with QTL regions) were selected from database and in silico searched for genetic variability and putative miRNA target sites in 3'UTR. Thirty-one SNPs in the putative promoter/5' UTR (up to 2 kb upstream) of eight candidate genes were found. Bioinformatic analysis revealed that some promoter SNPs might potentially cause gain/loss of the putative transcription factors. Promoter SNPs were alsopresent in CG dinucleotides and therefore possibly involved in gain/loss of CpG sites. In ten mastitis candidate genes we found 56 SNPs in exons of which 21 were non-synonymous substitutions. Additionally, 23 SNPs in intronic regions and 21 SNPs in 3'UTR were found. MiRNA target analysis revealed 89 putative target sites in 18 candidate genes; however, current SNPs were not identified in the miRNA target binding sites or miRNAs expressed in mammary gland. For SNPs with a putative regulatory role found in candidate genes, functional analyses and association studies are needed to facilitate identification of mastitis resistance or susceptibility alleles possibly involved in mastitis regulatory pathways"},{"@xml:lang":"sl","#text":"Pri iskanju kandidatnih genov ya mastitis smo s pomočjo petih različnih študijskih pristopov (QTL-i, asociacijske študije, ekspresijski eksperimenti, miRNA, AFLP študije in epigenetski faktorji) pregledali 233 lokusov. Da bi sestavili podatke iz različnih virov v smiselno celoto in razkrili prekrivajoče se regije, smo rezultate predstavili v obliki genske karte. Zbrani podatki predstavljajo genetsko ozadje za lastnosti povezane z mastitisom pri govedu. Trideset najbolj obetavnih kandidatov (povezanih z mastitisom v različnih študijskih pristopih ali z vsaj dvema neodvisnima študijama ali/in ležeče v regijah prekrivajočih se s QTL-i) smo izbrali iz podatkovne zbirke in uporabili za in silico iskanje potencialnih, z mastitisom povezanih, molekularnih markerjev in domnevnih tarčnih mest za miRNAv 3' neprevedenih regijah. (3'UTR). V domnevni promotorski/5'UTR (2kb navzgor od začetka prevajanja) smo našli 31 SNP-jev v osmih kandidatnih genih. Analiza z Matinspectorjem je pokazala, da bi nekateri SNP-ji v promotorskih regijah lahko povzročili izgubo/pridobitev vezavnih mest za transkripcijske faktorje. Nekateri SNP-ji v domnevni promotorski regiji povzročajo tudi izgubo/pridobitev CpG mest. V desetih kandidatnih genih smo našli 56 SNP-jev v eksonih, med katerimi je bilo 21 zamenjav ne-sinonimnih. Poleg tega smo našli 23 SNP-jev v intronskih regijah in 21 SNP-jev v 3'UTR. Analiza tarč za miRNA je razkrila 89 domnevnih vezavnih mest za miRNA v 18 kandidatnih genih. V tarčnih zaporedjih za miRNA nismo našli SNP-jev. Za SNP-je z domnevno regulatorno vlogo v kandidatnih genih predlagamo funkcionalne analize in asociacijske študije, ki bi omogočile odkrivanje alel za odpornost oz. dovzetnost za mastitis in prispevale k razumevanju regulatornih poti pri mastitisu"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-XZCYBMK3","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-XZCYBMK3"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-XZCYBMK3/b233fb5d-9111-49ea-9875-15032df72f7f/PDF"},"edm:rights":{"@rdf:resource":"http://rightsstatements.org/vocab/InC/1.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Univerza v Ljubljani, Biotehniška fakulteta"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-XZCYBMK3/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-XZCYBMK3"}}}}