23 KB172 KB20 KB19982023Microbiology2011Marinšek-Logar, RomanaNovak, DomenOsojnik Črnivec, Ilja GasanStres, BlažŠkrjanec, Igorštevilka:1letnik:58str. 171-175ISSN:1318-0207COBISSID:2832776URN:URN:NBN:SI:doc-AHS7LJXVenSlovenian Chemical SocietySlovensko kemijsko društvoActa chimica slovenicabiogorivobiomasabioplinmetanmicrobiologymikrobiologijapivske tropineGeneral microbial community flexibility in biochemical methane potential assay is highly correlated to initial biogas production ratesDegradation of brewery spent grain as a novel test substrate was explored in routine biochemical methane potential assays (BMP) using three different inocula. Significant differences in the initial biogas production rates from spent grain, methane yield coefficients and final spent grain degradation were observed between inocula. Initial and developed communities degrading novel substrate showed significant differences in archael community fingerprints. Differences were observed irrespective of substrate identity (no substrate, glucose, spent grain) providing evidence of a significant general influence of BMP incubation on the microbial phylotypes. A linear relationship between microbial community flexibility in BMP assay and corresponding initial biogas production rates was identified as a novel parameter to diagnose anaerobic processes, particularly under dynamic conditions like start-upZa preizkus razgradljivosti pivskih tropin kot novega substrata smo uporabili rutinski test biokemijskega metanskega potenciala (BMP). Med tremi različnimi biomasami smo opazili statistično signifikantne razlike v začetni hitrosti proizvodnje bioplina, deležu metana ter končni razgrajenosti pivskih tropin. Profili začetnih in končnih arhejskih mikrobnih združb so se medsebojno razlikovali ne glede na razlike v vrsti substrata (brez substrata, glukoza, pivske tropine). To kaže na pomeben splošen vpliv testa BMP na mikrobne skupine. Ugotovili smo linearen odnos med fleksibilnostjo mikrobnih združb v testu BMP in začetno hitrostjo proizvodnje bioplina, kar predstavlja nov parameter za diagnosticiranje anaerobnih procesov, predvsem v dinamičnih pogojih, kot je zagon reaktorjevTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko kemijsko društvo