416 KB21 KB199220251999Ihan, Alojzštevilka:1letnik:33str. 27-33ISSN:1318-2099COBISSID:9751769URN:URN:NBN:SI:doc-IDAHJ7E8enCroatian Medical Association - Croatian Society of RadiologySlovenian Medical Society - Section of RadiologyRadiology and oncology (Ljubljana)Cell degranulationcitotoksičnostCytotoxicity tests, immunologiceksocitozaEsterasesExocytosisfiziologijaFlow cytometryKiller cells, naturalLymphocytesSerineTumor cells, culturedtumorske celiceA correlation of NK cytolitic test and BLT esterase test in determining activity of NK cells, stimulated by tumor target cells| Korelacija med NK citolitičnim in BLT esteraznim testom pri določevanju aktivnosti NK celic stimuliranih s tumorskimi celicami|We examined the granule exocytosis in natural killer (NK) cells by measuring N-benzyloxycarboxy-L-lysine esterase activity. As a source of NK cells we usedbuffy-coat isolated NK cells or peripheral blood lymphocytes (PBL). The exocytosis was triggered by incubating cells with ionomycin/PMA or by NK cell susceptible tumour target cells K562. When we stimulated purified NK cells with tumour target cells, a close correlation (Corr=0.84) of cytolitic NK testresults and BLT test results was obtained. We may conclude that BLT test can provide a valuable tool to discriminate further NK cell deficiency in patients with low cytolitic NK test resultsZ meritvami encimske aktivnosti N-benzyloxycarboxy-L-lizinske esteraze smo preiskovali eksoitozo celic NK. Celice NK smo osamili iz levkocitnega koncentrata (Buffy coat) oz. venske krvi. Eksocitozo smo sprožili s kombinacijo PMA/ionomcin ali s tumorskimi celicami K542. Ob stimulaciji celic NK s tumorskimi celicami (K542) smo opazovali značilno soodvisnost (Cor=0.H4) med rezultati citolitične aktivnosti celic NK in med izmerjenimi encimskimi aktivnosti N-benzv- loxycarboxy-L-lizinske esteraze (BLT test). Ugotavljamo, da BLT test omogoča nadrobnej5i študij dogodkov, ki se odvijajo med citotoksično aktivnostjo celic NKTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaOnkološki inštitut Ljubljana