414 KB19 KB199220251999Čemažar, MajaLavrič, MiraMarolt, DarjaSerša, Gregorletnik:33številka:4str. 303-308COBISSID_HOST:11071449ISSN:1318-2099URN:URN:NBN:SI:doc-L31RX9NGenCroatian Medical Association - Croatian Society of RadiologySlovenian Medical Society - Section of RadiologyRadiology and oncology (Ljubljana)CisplatinColorimetryDrug therapyFibrosarcomaMiceRadiotherapySarcoma, experimentalTetrazolium saltsTumor stem cell assayComparison of colorimetric MTT and clonogenic assays for irradiation and cisplatin treatment on murine fibrosarcoma SA-1 cells| Primerjava kolorimetričnega testa MTT in testa klonogenosti na mišjih fibrosarkomskih SA-1 celicah po obsevanju in zdravljenju s cisplatinom|Background. The aim of our study was to determine the relationship between cell survival of SA-1 tumor cells measured by clonogenic assay and MTT assay after irradiation and cisplatin treatment. Materials and methods. Survival of SA-1 cells was measured after irradiation (2-8 Gy) and cisplatin treatment (0.05-0.5 u g/ml) by clonogenic assay performed 7 days after treatment, and byMTT assay performed on day 3, 4, 5, and 7 after the treatment. Results. The results showed good correlation between MTT assay and clonogenic assay for irradiation doses below 4 Gy. For higher doses good correlation between MTT and clonogenic assay was determined only when MTT assay was performed on day 5and 7 after the treatment. In the case of cisplatin treatment, similar pattern was observed, good correlation between IC50 values for MTT and clonogenic assay was found when MTT assay was performed on day 5 and 7 after the treatment. Conclusion. Results of our study confirmed the results of previous studies addressing this topic and further support the use of MTT testas an alternative test for clonogenic test as a predictive assay of tumourresponse to the radio or chemotherapyIzhodišče. Namen naše raziskave je bil določiti povezavo med testom MTT in testom klonogenosti z merjenjem preživetja SA-1 tumorskih celic po obsevanju in po terapiji s cisplatinom. Materiali in metode. Preživetje SA-1 celic smo določali po obsevanju celic (2-8 Gy) ali po terapiji s cisplatinom (0.05 - 0.5ug/ml) s testom klonogenosti, ki smo ga izvedli 7 dan po terapiji, ter testom MTT, ki smo ga izvedli 3., 4., 5., in 7. po terapiji. Rezultati. Ugotovili smo, da testa dobro korelirata, ko smo celice obsevali z dozami pod 4 Gy. Pri višjih dozah obsevanja sta test MTT in test klonogenosti dobro korelirala samo v primeru, ko smo merili preživetje celic z testom MTT 5. in 7. dan po obsevanju. Po terapiji s cisplatinom smo dobili podobne rezultate; korelacija med testom MTT in testom klonogenosti je bila dobra v primeru, ko smo izvedli test MTT 5. in 7. dan po terapiji. Zaključki. Rezultati naše raziskave so potrdili ugotovitve predhodnih študij, ter podpirajo uporabo testa MTT kot alternativo testu klonogenosti pri napovedovanju izida zdravljenja tumorjev z radio- in kemoterapijoTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaOnkološki inštitut Ljubljana