742 KB21 KB199220251998Garaj-Vrhovec, VeraKopjar, Nevenkaletnik:32številka:4str. 385-392ISSN:1318-2099COBISSID_HOST:8141785URN:URN:NBN:SI:doc-RB2Y9N96enCroatian Medical Association - Croatian Society of RadiologySlovenian Medical Society - Section of RadiologyRadiology and oncology (Ljubljana)Adverse effectsAntineoplastic agentsLymphocytesMicronucleus testsOccupational exposureMicronuclei in cytokinesis-blocked lymphocytes as an index of occupational exposure to antineoplastic drugs| Mikronukleus test pri osebah s poklicno izpostavljenostjo citostatikom|In order to investigate possible DNA damiging effects of antineoplastic drugs,the micronucleus assay combined with Giemsa, DAPI and silver staining was performed. Blood samples were taken from nurses working without adequate protection in oncological department on preparing and administration of different antineoplastic drugs. Lymphocytes were cultivated in vitro at 37 degrees C. To prevent cytokinesis, at 44th cytochalasine-B at a final concentration of 3 micro g/ml was added. The results obtained indicate statistically significant increases in the total number of micronuclei in the exposed subjects compontrols. DAPI staining has revealed signal-positive and signal-negative miuclei while silver staiing has revealed Ag-NOR+ and Ag-NOR- micronuclei. Compred to controls, the number of signal-positive exposed subjects were increased, indicating a greater susceptibility of particular chromosomes to damage caused by antineopalstic agentsAvtorji so s pomočjo mikronukleus testa v kombinaciji z različnimi tehnikami barvanja (Giemsa, DAPI in srebro) proučevali možne poškodbe DNK zaradi delovanja citostatikov. Krvne vzorce za preiskave so jemali medicinskim sestram, ki so na onkoloških oddelkih brez ustrezne zaščite opravljale delo v zvezi s pripravo in aplikacijo različnih citostatikov. Limfocite so gojili in vitro pri temperaturi 37 degrees C. Za preprečitev citokineze so po 44 urah dodali citohalazin B v končni koncentraciji 3 micro g/ml. Dobljeni rezultati kažejo statistično značilen porast skupnega števila mikronukleusov pri izpostavljeni skupini v primerjavi s kontrolno skupino. Z DAPI tehniko so prikazali signal-pozitivne in signal-negativne mikronukleuse, medtem ko je tehnika bavanja s srebrom razkrila Ag-NOR-pozitivne in Ag-NOR-negativne mikronukleuse. V primerjavi s kontrolno skupino je bilo število signal-pozitivnih in Ag-NOR-pozitivnih nukleusov pri izpostavljenih osebah zvišano, kar kaže, da so nekateri kromosomi bolj dovzetni za poškodbe, ki jih povzročajo citostatikiTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaOnkološki inštitut Ljubljana