{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-TM0KRB5O/729e06a5-2b7e-4cf0-a7c8-9fc47492accd/PDF","dcterms:extent":"298 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-TM0KRB5O/b5fdc32c-5585-4f51-8814-193eb73c106e/TEXT","dcterms:extent":"0 KB"}],"edm:TimeSpan":{"@rdf:about":"1929-2026","edm:begin":{"@xml:lang":"en","#text":"1929"},"edm:end":{"@xml:lang":"en","#text":"2026"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-TM0KRB5O","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/urn:nbn:si:spr-a30mfzkp"},{"@xml:lang":"sl","#text":"Zdravniški vestnik"}],"dcterms:issued":"2019","dc:creator":["Bricl, Irena","Dovč, Tadeja","Mrak, Janja","Železnik, Klara"],"dc:format":[{"@xml:lang":"sl","#text":"številka:11/12"},{"@xml:lang":"sl","#text":"letnik:88"},{"@xml:lang":"sl","#text":"str. 582-592"}],"dc:identifier":["ISSN:1318-0347","COBISSID_HOST:34670041","URN:URN:NBN:SI:doc-TM0KRB5O"],"dc:language":"sl","dc:publisher":{"@xml:lang":"sl","#text":"Slovensko zdravniško društvo"},"dc:subject":[{"@xml:lang":"sl","#text":"parcialni D"},{"@xml:lang":"en","#text":"partial D"},{"@xml:lang":"sl","#text":"šibki D"},{"@xml:lang":"sl","#text":"variante D"},{"@xml:lang":"en","#text":"variants D"},{"@xml:lang":"en","#text":"weak D"}],"dcterms:temporal":{"@rdf:resource":"1929-2026"},"dc:title":{"@xml:lang":"sl","#text":"Obravnava nosečnic glede na tip RhD| Treatment of pregnant women by RhD type|"},"dc:description":{"@xml:lang":"sl","#text":"An accurate determination of RhD (D) antigen (Ag) is important for pregnant women, transfusion recipients and blood donors. The decision to receive the pre- and postnatal prophylaxis with anti-D immunoglobulin (RhIG) is based on the D-type. Individuals can be classified as D-posi- tive (D-pos) or D-negative (D-neg) based on the determination of Ag D but there are numerous other versions of Ag D as well (variants D, D-var). Allele variants of the RHD (D) gene can encode different forms of protein D. They are divided into weak, partial and D el . Ag D are determined on the basis of serological techniques. If in doubt, we use the molecular-biological methods for determining the D gene. The definitive definition of D-var is given on the basis of the molecular - -biological methods . From the peripheral venous blood samples taken from pregnant women, we roughly determi- ned Ag D on plates (the Seraclone anti-D reagent) and on gel cards (the DiaClon ABO / D + Re verse Grouping test system (monoclonal antibodies) (Bio-Rad, Germany), with which we do not detect D category VI blood group). For 49 pregnant women we performed extended serological tests of D blood group (commercial ID-Partial RhD Typing Set (Bio-Rad, Germany)). We procee - ded with molecular-biological methods: DNA isolation (utilisation of the BioRobot EZ1 and the commercial set EZ1 DNA Blood 350 %l Kit (Qiagen, Germany)), determination of the genotype D with the PCR-SSP method (commercial RBC-Ready Gene CDE, RBC -Ready Gene D weak and RBC-Ready Gene D AddOn (Inno-Train; Germany)), amplification (Veriti apparatus (Apllied Bio - Systems, USA)), product separation (electrophoretic system (BioRad, USA) on agarose gel (Si- gma, Germany). Our results showed that weak D forms represented 41 cases (83.7 %) and partial forms 8 cases (16.3 %) of all D-var. The most common D-var in pregnant women was weak D type 1 with 17 ca- ses (34.7 %), followed by weak D type 3 with 13 cases (26.5 %) and weak D type 2 with 10 cases (20.4 %). The most common partial D form was D category VII with 5 cases (10.2 %). Pregnant women and transfusion recipients who are carriers of weak D types 1, 2 or 3 can be safely treated as D-pos, while carriers of all other D-var can be treated as D-neg. Blood donors with D-var are treated as D-pos. By means of this algorithm, approximately 172 unnecessary RhIG applications can be prevented annually and adequate supplies of D-neg erythrocyte blood com- ponents can be maintained"},"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-TM0KRB5O","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-TM0KRB5O"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-TM0KRB5O/729e06a5-2b7e-4cf0-a7c8-9fc47492accd/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by-nc/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko zdravniško društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-TM0KRB5O/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-TM0KRB5O"}}}}