22 KB480 KB21 KB192920262010Ihan, AlojzRylander, RagnarSalobir, BarbaraSimčič, SašaStopinšek, SanjaTerčelj-Zorman, MarjetaWraber-Herzog, Brankaštevilka:106 straniletnik:79str. 684-689ISSN:1318-0347COBISSID:27497433URN:URN:NBN:SI:doc-U1KPQ1APenSlovensko zdravniško društvoZdravniški vestnikCelična stenaCell WallChitindejavniki tveganjadiagnostikaFungifungusGliveHitinimunopatogenezaLeukocytes, MononuclearLevkociti mononuklearniLipopolisaharidiLipopolysaccharidesmicrobiologymikrobiologijaPolisaharidiPolysaccharidesSarcoidosisSarkoidozaTumor Necrosis FactorTumor nekrotizirajoči faktorEffects of fungal cell wall polysaccharides and lipopolysaccharide on in vitro tumour necrosis factor alpha production by peripheral blood mononuclear cells of sarcoidosis patients| Učinki polisaharidov iz celične stene gliv in lipopolisaharida na in vitro sintezo tumorskega nekroznega dejavnika alfa v mononuklearnih celicah iz periferne krvi bolnikov s sarkoidozo| Učinki polisaharidov iz celične stene gliv in lipopolisaharidana in vitro sintezo tumorskega nekroznega dejavnika alfa v mononuklearnih celicah iz periferne krvi bolnikov s sarkoidozo|Background: Living in damp and moldy environments is being associated with a high risk for sarcoidosis and recently fungi have been suspected to be involved in the immunopathogenesis of sarcoidosis. We developed a model to study the influence of fungal cell wall polysaccharides on in vitro cytokine production by peripheral blood mononuclear cells (PBMC). We investigated whether different fungal cell wall polysaccharides induce different levels of tumour necrosis factor alpha (TNF-alpha) production by PBMC. We also assessed whether fungal cell wall polysaccharides modulate the lipopolysaccharide (LPS)- induced the TNF-f production by PBMC. And finally, we assessed whetherthere is a possible difference in TNF-f response of PBMC between patients with sarcoidosis and healthy persons. Methods: Eleven patients with newly diagnosed sarcoidosis and eleven healthy volunteers were included in thestudy. PBMC were isolated from whole blood and stimulated with fungal cell wall polysaccharides (soluble and particulate(1->3)-beta-glucan, zymosan A andchitin) and LPS. The secretion of TNF-alfa was measured from cultured PBMC.Results: Particulate (1->3)-beta-glucan was a potent inducer of TNF-alphasecretion by PBMC from patients with sarcoidosis and healthy persons.Soluble (1->3)-beta-glucan, zymosan A and chitin were weak inducers ofTNF-alfa secretion by PBMC from patients with sarcoidosis and healthy persons. Soluble (1->3)-beta-glucan increased, while particulate (1->3)-beta-glucan and chitin depressed the LPS induced secretion of TNF-á by PBMC from patients with sarcoidosis and healthy persons. There was no significant difference between patients with sarcoidosis and healthy persons, although there was a slight decrease of TNF-alpha secretion by PBMC from patients with sarcoidosis, compared to healthy persons. Conclusions: In this article we present the results of our preliminary study. (Abstract truncated at 2000 characters)TEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko zdravniško društvo