{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-W072NVCM/e062b75f-e578-4516-a950-704f54a34c43/PDF","dcterms:extent":"113 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-W072NVCM/9833d76c-5394-4b47-922e-4872725a1379/TEXT","dcterms:extent":"23 KB"}],"edm:TimeSpan":{"@rdf:about":"1929-2026","edm:begin":{"@xml:lang":"en","#text":"1929"},"edm:end":{"@xml:lang":"en","#text":"2026"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-W072NVCM","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/urn:nbn:si:spr-a30mfzkp"},{"@xml:lang":"sl","#text":"Zdravniški vestnik"}],"dcterms:issued":"2004","dc:creator":["Černelč, Peter","Ljutić, Elvira","Pajič, Tadej"],"dc:format":{"@xml:lang":"sl","#text":"str. I-9-I-12"},"dc:identifier":["ISSN:1318-0347","COBISSID_HOST:17634265","URN:URN:NBN:SI:doc-W072NVCM"],"dc:language":"sl","dc:publisher":{"@xml:lang":"sl","#text":"Slovensko zdravniško društvo"},"dc:subject":[{"@xml:lang":"sl","#text":"Chromosome Aberrations"},{"@xml:lang":"sl","#text":"Diagnosis"},{"@xml:lang":"sl","#text":"Electrophoresis, Agar Gel"},{"@xml:lang":"sl","#text":"Elektroforeza, agar gel"},{"@xml:lang":"sl","#text":"Genetics"},{"@xml:lang":"sl","#text":"Kromosomske aberacije"},{"@xml:lang":"sl","#text":"Leukemia"},{"@xml:lang":"sl","#text":"Leukemia, B-Cell, Acute"},{"@xml:lang":"sl","#text":"Leukemia, Lymphocytic, Acute"},{"@xml:lang":"sl","#text":"Leukemia, Myeloid, Chronic"},{"@xml:lang":"sl","#text":"Leukemia, T-Cell, Acute"},{"@xml:lang":"sl","#text":"Levkemija"},{"@xml:lang":"sl","#text":"Levkemija B-celična, akutna"},{"@xml:lang":"sl","#text":"Levkemija limfocitna, akutna"},{"@xml:lang":"sl","#text":"Levkemija mieloidna, kronična"},{"@xml:lang":"sl","#text":"Levkemija T-celična, akutna"},{"@xml:lang":"sl","#text":"Polimerazna, verižna reakcija"},{"@xml:lang":"sl","#text":"Polymerase Chain Reaction"},{"@xml:lang":"sl","#text":"Reagent Kits, Diagnostic"},{"@xml:lang":"sl","#text":"Reagentni kompleti diagnostični"}],"dcterms:temporal":{"@rdf:resource":"1929-2026"},"dc:title":{"@xml:lang":"sl","#text":"Prepoznavanje značilnih kromosomskih prireditev z reagenčnim kompletom mDx HemaVision pri bolnikih z levkemijo| Detection of specific chromosomal rearrangement in leukemia patients by mDx HemaVision kit|"},"dc:description":[{"@xml:lang":"sl","#text":"Background. The mDx HemaVision kit is a qualitative multiplex and nested Reverse Transcription. Polymerase Chain Reaction (RTPCR) test designed to detect 28 different tra`aslocations or chromosomal rearrangement, found to be specific for particular subtypes of leukemia. The presen.ce or absence of the specific mRNA transcripts or cDNA segments after the reverse transcription of the fusion or abnormal genes, appeared after chromosomal rearrangements, couldbe determined by the kit. Patients and methods. The usefulness of the kitwas tested on the 26 RNA sarnples of patients with acute leukemia and sevenpatients with chronic mieloproliferative diseases and by comparison of the results between mDx HemaVision kit and standardized RT-PCR protocol for the specific mRNA transcripts of the t(9;22)(q34;q11), t(8;21)(q22;q22), t(15;17)(q22;q22) and t(4;11)(q21;q23). The RNA samples were isolated from mononuclear cells of the bone marrow after Ficoll-Paque density gradient centrifugation and with a High Pure RNA isolation kit. The cDNA synthesis and Polymerase Chain Reaction were performed as described in mDx HemaVisoin's or standardized RT-PCR's protocols. The PCR products were analyzed by agarose gelelectrophoresis, by staining with etidium bromide and by visualization under UV light. Results. We obtained 100% concordance of the results by both methods. Specific BCR-ABL mRNA transcripts were found in four chronic myeloid leukemia patients, one in B acute lymphoblastic leukemia (B-ALL) and one with bifenotypic leukemia (BAL) patient. AML1-ETO mRNA transcript of the t(8;21)(q22;q22) was identified in two patients with acute myeloid leukemia (AML). The CBFbeta/MYH11 mRNA transcript specific for invl6(p13;q22) was obtained in AML patient with abnormal eozinophiles in bone marrow. MLL/AF4 mRNA transcript of the t(4;11)(q21;q23) was found in the girl with BALL and inpatient with B-ALL after treatment. (Abstract truncated at 2000 characters)"},{"@xml:lang":"sl","#text":"Izhodišča. Reagenčni komplet mDx HemaVision je namenjen za kvalitativno ugotavljanje 28 različnih kromosomskih preureditev, značilnih za posamezne podvrste levkemij z načinom obratnega prepisovanja, hkratne in vgnezdene verižne reakcije s polimerazo. S to reakcijo lahko določimo prisotnost ali odsotnost specifičnih mRNK prepisov oziroma cDNK segmentov po obratnem prepisovanju združenih ali okvarjenih genov, ki nastanejo zaradi kromosomskih preureditev. Bolniki in metode. Uporabnost reagenčnega kompleta smo preverili v 26 vzorcih RNK bolnikov z akutno levkemijo in 7 s kronično mieloproliferativno boleznijo ter primerjali izsledke med reagenčnim kompletommDx HemaVision in usklajenim protokolom RT-PCR pri ugotavljanju značilnih prepisov mRNK t(9;22)(q34;q11), t(8;21)(q22;q22), inv16(p13;q22), t(15;17)(q21;q21) in t(4;11)(q21;q23). RNK smo pridobili iz enojedrnih celic kostnega mozga po gostotnem gradientnem centrifugiranju preko fikola in z uporabo reagenčnega kompleta High Pure RNA Isolation. Sintezo komplementarne DNK in verižno reakcijo s polimerazo smo izvedli, kot je opisano v protokolu reagenčnega kompleta ali mDx HemaVision v usklajenem protokolu RT-PCR. Pridelke PCR reakcij smo ocenjevali z agarozno gelsko elektroforezo, barvanjemgelov z etidijevim bromidom in detekcijo pod UV lučjo. Rezultati. Dobili smo 100-odstotno ujemanje izsledkov za preiskave, ki so skupne obema postopkoma. Značilen BCRABL mRNK prepis t(9;22)(q34;q11) smo dokazali pri bolniku z BALL, bolniku z bifenotipsko levkemijo in pri štirih bolnikih s KML.AHL1-ETO mRNK prepis t(8;22)(q22;q22) smo ugotovili pri dveh bolnikih z AML. Pri enem bolniku z AML z nenormalnimi eozinofilci v kostnem mozgu smo dokazali CBFbeta/ MYH11 mRNK prepis inv16(p13;q22). MLL/AF4 mRNK prepis t(4,11))(q21;q23) smo ugotovili pri deklici z B-ALL in bolniku z BALL po zdravljenju s citostatiki. (Izvleček skrajšan pri 2000 znakih)"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-W072NVCM","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-W072NVCM"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-W072NVCM/e062b75f-e578-4516-a950-704f54a34c43/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by-nc/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko zdravniško društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-W072NVCM/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-W072NVCM"}}}}