{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-WFP0IQX7/b59d6d0d-60e5-466a-a4f1-4b99e6f7a2fb/PDF","dcterms:extent":"1198 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:doc-WFP0IQX7/f6c6dd03-8a65-41e4-9acc-08b69cd8dba9/TEXT","dcterms:extent":"0 KB"}],"edm:TimeSpan":{"@rdf:about":"2013-2025","edm:begin":{"@xml:lang":"en","#text":"2013"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:doc-WFP0IQX7","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-MCCAWXYE"},{"@xml:lang":"sl","#text":"Acta silvae et ligni"}],"dcterms:issued":"2023","dc:creator":["De Groot, Maarten","Devetak, Zina","Kavčič, Andreja","Piškur, Barbara"],"dc:format":[{"@xml:lang":"sl","#text":"številka:132"},{"@xml:lang":"sl","#text":"str. 29-38"}],"dc:identifier":["DOI:10.20315/ASetL.132.3","COBISSID:180304131","ISSN:2335-3112","URN:URN:NBN:SI:doc-WFP0IQX7"],"dc:language":"en","dc:publisher":[{"@xml:lang":"sl","#text":"Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire"},{"@xml:lang":"sl","#text":"Biotehniška fakulteta, Oddelek za lesarstvo"},{"@xml:lang":"sl","#text":"Gozdarski inštitut Slovenije, založba Silvae Slovenica"}],"dc:subject":[{"@xml:lang":"en","#text":"early detection"},{"@xml:lang":"sl","#text":"ekstrakcijski postopek"},{"@xml:lang":"en","#text":"molecular diagnostics"},{"@xml:lang":"sl","#text":"molekularna diagnostika"},{"@xml:lang":"sl","#text":"PCR"},{"@xml:lang":"sl","#text":"polimerazna verižna reakcija"},{"@xml:lang":"en","#text":"polymerase chain reaction"},{"@xml:lang":"en","#text":"Sanger sequencing"},{"@xml:lang":"sl","#text":"sekvenciranje po Sangerju"},{"@xml:lang":"sl","#text":"shranjevanje osebkov"},{"@xml:lang":"en","#text":"specimen storage"},{"@xml:lang":"en","#text":"total DNA extraction"},{"@xml:lang":"sl","#text":"zgodnje zaznavanje"}],"dcterms:temporal":{"@rdf:resource":"2013-2025"},"dc:title":{"@xml:lang":"sl","#text":"The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.)| Vpliv postopkov shranjevanja in ekstrakcije celokupne DNA na rezultate molekularne analize osmerozobega smrekovega lubadarja (Ips typographus L.)|"},"dc:description":[{"@xml:lang":"sl","#text":"One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process"},{"@xml:lang":"sl","#text":"Za uspeh molekularne identifikacije podlubnikov je ključna zadostna količina kakovostnega ekstrakta DNA. Pri raziskavi vrste smo se osredotočili na vpliv različnih postopkov shranjevanja osebkov Ips typographus(L.) in različnih protokolov ekstrakcije DNA na količino in kakovost DNA, namenjene za uporabo v molekularni diagnostiki. Odrasle hrošče smo zamrznili pri -20 °C na dva načina, v etanolu in na suho. Ekstrakcije smo opravili po štirih različnih postopkih. Primerjali smo količino pridobljene DNA. Kakovost ekstrahirane DNA smo ocenili s polimerazno verižno reakcijo in sekvenciranjem po Sangerju. Način shranjevanja ni statistično značilno vplival na količino pridobljene DNA. DNA višje kakovosti smo pridobili iz hroščev, ki so bili zamrznjeni v etanolu. Le dva postopka ekstrakcije DNA sta dala DNA, uporabno za sekvenciranje. V količini pridobljene DNA se pri sekvenciranju uspešna postopka nista pomembno razlikovala. Določili smo optimalno kombinacijo postopkov shranjevanja v etanolu pri -20 °C in ekstrakcije celokupne DNA z ekstrakcijskim kitom Nucleospin Insect DNA proizvajalca Macherey Nagel, ki omogoča časovno učinkovito identifikacijo"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:doc-WFP0IQX7","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:doc-WFP0IQX7"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:doc-WFP0IQX7/b59d6d0d-60e5-466a-a4f1-4b99e6f7a2fb/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by-sa/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Gozdarski inštitut Slovenije"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:doc-WFP0IQX7/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:doc-WFP0IQX7"}}}}