1399 KB15 KB199220251998Breskvar, KatjaDolžan, VitaRavnik-Glavač, Metkaštevilka:1letnik:32str. 35-39ISSN:1318-2099COBISSID:7555289URN:URN:NBN:SI:doc-WKM4NJLAenCroatian Medical Association - Croatian Society of RadiologySlovenian Medical Society - Section of RadiologyRadiology and oncology (Ljubljana)Colorectal neoplasmsCytochrome p-450 cyp1a1Cytochrome p-450 cyp2d6encimifiziologijaGeneticsgenetski polimorfizemGlutathione transferasesksenobiotikiPolymerase chain reactionPolymorphism (genetics)presnovarak (medicina)Genetic polymorphisms of xenobiotic metabolizing enzymes in human colorectal cancer|It was proposed that both hereaditary and environmental factors contribute to the development of colorectal cancer (CRC). Carcinogenic polycyclic aromatic hydrocarbons (PAHs) from food or tobacco smoke can form DNA adducts and thus initiate carcinogenesis after metabolic activation via cytochrome P4501A1 (CYP1A1). Intermediate metabolites are detoxified by conjugation with glutathione S-transferases. Our aim was to look for inherited metabolic suceptibility to CRC. We used PCR-based genotyping approach to determine the frequencies of polymorphic alleles of two cytochromes P450 (CYP2D6 and CYP1A1)and two glutathione S-transferases (GSTM1 and GSTT1) in DNA samples from 31 sporadic, 25 familial CRC cases and 73 healthy controls. The difference in frequencies of poor metabolisers due to CYP2D6 gene polymorphismwas close to the limit of statistical significance between sporadiCRC and healthy control group (lambda 2=5.52, m=2, p=0.06) despite the small sample size. The frequencies of either CYP1A1 MspI, GST M1 or GST T1 genotypes were not significantly different in both CRC cases and in controls. Although our study suggests some difference in metabolic susceptibility between sporadic and familial CRC, further studies are needed to investigate the combined effect of polymorphic genes involved in carcinogen metabolism in a larger group of patients with defined exposure to dietary carcinogens and smokingTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaOnkološki inštitut Ljubljana