232 KB52 KB192920262015Ambrožič, AlešBožič, BorutČučnik, SašaŽigon, Polonaštevilka:3letnik:84str. 209-221ISSN:1318-0347COBISSID:32034265URN:URN:NBN:SI:doc-YYW4AVJAslSlovensko zdravniško društvoZdravniški vestnikantifosfolipidna protitelesaantifosfolipidni sindromanti-phosphatidylserine prothrombin antibodiesantiphospholipid antibodiesantiphospholipid syndromeencimsko imunska metodaprotitelesaProtitelesa proti protrombinu| Antiprothrombin antibodies|In patients with the antiphospholipid syndrome (APS), the presence of a group of pathogenic autoantibodies called antiphospholipid antibodies causes thrombosis and pregnancy complications. The most frequent antigenic target of antiphospholipid antibodies are phospholipid bound beta2-glycoprotein 1 (beta2GPI) and prothrombin. The international classification criteria for APS connect the occurrence of thrombosis and/or obstetric complications together with the persistence of lupus anticoagulant, anti-cardiolipin antibodies (aCL) and antibodies against beta2GPI (anti-beta2GPI) into APS. Current trends for the diagnostic evaluation of APS patients propose determination of multiple antiphospholipid antibodies, among them also anti-prothrombin antibodies, to gain a common score which estimates the risk for thrombosis in APS patients. Antiprothrombin antibodies are common in APS patients and are sometimes the only antiphospholipid antibodies being elevated. Methods for their determination differ and have not yet been standardized. Many novel studies confirmed method using phosphatidylserine/prothrombin (aPS/PT ELISA) as an antigen on solid phase encompass higher diagnostic accuracy compared to method using prothrombin alone (aPT ELISA). Our research group developed an in-house aPS/PT ELISA with increased analytical sensitivity which enables the determination of all clinically relevant antiprothrombin antibodies. aPS/PT exhibited the highest percentage of lupus anticoagulant activity compared to aCL and anti-beta2GPI. aPS/PT antibodies measured with the in-house method associated with venous thrombosis and presented the strongest independent risk factor for the presence of obstetric complications among all tested antiphospholipid antibodiesTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko zdravniško društvo