58 Research Article Aljaž Jakob 1,2,†, Urška Kuhar 3†, Miloš Vittori 1†, Tina Klenovšek 4* Simona Strgulc Krajšek 1* Abstract In this paper, we present two significant records of mosses in Slovenia: Bryum canariense and Fissidens fontanus, and the first record of an animal species, Porcellio obsoletus, from the Slovene Coast. Keywords Bryum canariense; Fissidens fontanus; Porcellio obsoletus; bryophytes; mosses, flora; fauna; Isopoda; isopods; Slovenia 1 University of Ljubljana, Biotechnical Faculty, Department of Biology, Večna pot 111, 1000 Ljubljana, Slovenia 2 ZRC SAZU, Jovan Hadži Institute of Biology, Novi trg 2, 1000 Ljubljana, Slovenia 3 Ministrstry of Environment, Climate and Energy, Slovenian Environment Agency, Vojkova 1b, SI-1000 Ljubljana, Slovenia 4 University of Maribor, Faculty of Natural Sciences and Mathematics, Maribor, Slovenia * Editors For correspondence, see the individual chapters. † These authors contributed equally to this work. Citation: Jakob, A., Kuhar, U., Vittori, M., Klenovšek, T., Strgulc Krajšek, S. (2024). Significant records of plants, algae, fungi, and animals in SE Europe and adjacent regions 2. Acta Biologica Slovenica, 67 (2) https://doi.org/ 10.14720/abs.67.2.19618 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY SA) license Significant records of plants, algae, fungi, and animals in SE Europe and adjacent regions, 2 59 Acta Biologica Slovenica, 2024, 67 (2) Bryum canariense is a data deficient species of Slovenian flora, previously only collected and reported in 1882 from Orleška draga (Martinčič, 2024). The New site lies 4 km southeast of the historical site at Orleška Draga in similar vegetation type (thermophilous Quercus pubescens forests with admixture of Pinus nigra in rather early stages of succession). B. canariense is a Mediterranean-Atlantic species (Hill et al., 2007), occurring in Europe from Great Britain in the north to the Mediterranean basin and Madeira in the South and from the Azores to Turkey. It is absent from some Mediterranean islands. In the immediate vicinity of the presented locality, it grows in Friuli-Venezia Giulia (Italy) and Croatia but is absent from Austria and Hungary. Its conservation status is least concern in Europe (Hogetts & Lockhart, 2020). The abandonment of land use from the late 19th century to the present is unfavourable for this moss. Previously, the area was predominantly a stony, open grassland and current reforestation (Kaligarič & Ivajnšič, 2014) of the area is likely reducing the habitats suitable for this heliophilic species of open spaces (Hill et al., 2007). B. canariense differs from all other species of Bryaceae by its arrangement of leaves into 2–3 comal tufts, where each year's rosette is placed above the last year's rosette. Some other rosulate species are similar, but all lack this arrangement, for example, the species of Rhodobryum have stolons, so tufts are not arranged above each other. Ptychostomum capillare and P. torquescens are smaller and lack a serrate border of the leaf tip, and the dry leaves of P. capillare are often strongly spirally twisted (erect at B. canariense). The leaves of both Ptychostomum species have a stronger border up to the leaf tip, sometimes with small teeth, whereas B. canariense has a weak border that usually disappears in the upper quarter of the leaf, where it is replaced by strong teeth (Casas et al., 2006; Holyoak, 2013). Funding This research was funded by the Slovenian Research and Innovation Agency, grant numbers MR-55879 and P1-0236. Contributor(s) Aljaž Jakob Corresponding author Aljaž Jakob (aljaz.jakob@zrc-sazu.si) Leg. Aljaž Jakob Country Slovenia Statement of significance The first recent record of a data deficient species in Slovenia. Locality description Primorska region, Classical Karst, Lipica, 390 m a. s. l. Habitat Quercus pubescens forest with admixed Pinus nigra, on the forest floor under Juniperus communis Date of observation 2024-03-25 GPS N 45.66687°, E 13.86696° Voucher Bryum canariense Brid., fam. Bryaceae (moss) Author’s personal herbarium, 203888293 60 Acta Biologica Slovenica, 2024, 67 (2) Fissidens fontanus is an aquatic moss species growing on submerged rocks or wood in mesotrophic lowland lakes and rivers (Frey et al., 2006). It has remote and narrow leaves, which are much longer than the leaves of other representatives of the genus. The upper one-layered part of the leaf is up to 3 times as long as the sheating part, the leaf is unbordered, and the costa ends before the apex (Frey et al., 2006; Godfrey, 2010). F. fontanus is an European temperate species (Hill & Preston, 1998), distributed from the Mediterranean to S. Scandinavia (Frey et al., 2006). It is also present in North America and in the Southern Hemisphere (Hill & Preston, 1998). The species is considered least concern (LC) in the European Red List (Hodgetts et al., 2019). It is present in all neighbouring countries of Slovenia except Hungary (Hodgetts & Lochhart, 2020; Aleffi et al., 2020; Alegro et al., 2019). In Slovenia, it has been only reported for the sub-Pannonial phytogeographical region, where it was found in 2018 in river Dravinja near Makole by Mar- tinčič (Martinčič, 2024), so our records are the first in the pre-Alpine phytogeographical region of Slovenia. The specimens in the river Ljubljanica and the channel Gruberjev prekop were found during the monitoring of macrophytes for the assessment of the ecological status of surface waters. Macrophytes were sampled in 100 m long river stretch by wading across the channel or from the shore or from the boat when wading is not possible. Funding Slovenian Environment Agency financially supported the monitoring of macrophytes. Contributor(s) Simona Strgulc Krajšek, Urška Kuhar Corresponding author Simona Strgulc Krajšek (simona.strgulc@bf.uni-lj.si) Leg. Urška Kuhar Country Slovenia Statement of significance The second and third known localities in Slovenia, the first two in Central Slovenia. Locality description (1) Central Slovenia, Ljubljana, Moste, Ljubljanica river, between the bridge of Grablovičeva street and the pedestrian bridge connecting Zaloška street and street Ob Ljubljanici, 300 m a.s.l. (2) Central Slovenia, Ljubljana, water channel Gruberjev prekop, between the bridge of Kajuhova street and the pedestrian bridge Mekinčeva brv, 300 m a.s.l. Habitat Submersed in the water on a rock substrate Date of observation 2022-07-25 GPS (1) N 46.055172, E 14.533501 (approximate coordinates) (2) N 46.051042, E 14.540049 (approximate coordinates) Voucher Herbarium LJU (s.n.) Fissidens fontanus (P. Karst.) Bas, fam. Fissidentaceae (moss) 61 Acta Biologica Slovenica, 2024, 67 (2) This is the first report of the occurrence of Porcellio obsoletus Budde-Lund, 1885 in Slovenia. The species was observed in a synanthropic habitat on the Slovenian coast. A single adult P. obsoletus female was spotted at night in a narrow street in the town of Piran in June 2019. The individual was collected and preserved in 96% ethanol. The species can be recognized by its weakly granulated tergites (Figure 1a), the position of the glandular pore fields on the anterior edges of pereon epimera (Figure 1b), a furrow running along articles four and five of the second antenna (Figure 1c), and broad, flat uropod exopodites (Strouhal, 1968). P. obsoletus is distributed in the central and eastern Mediterranean region, Iran, and the Crimea (Schmalfuss, 2003). In the territory of former Yugoslavia, it has previously been reported from Bosnia and Herzegovina as well as from the Croatian coast and islands (Dollfus, 1895; Rogenhofer, 1908; Potočnik, 1989). It has not yet been reported this far north in the Adriatic region. Given the proximity of its known distribution area, there is no reason to assume that its presence in Slovenia is due to human introduction. Previously, six species of Porcellio were known to occur in Slovenia (Vittori et al., 2023), making P. obsoletus the seventh recorded species of the genus and raising the total number of known Oniscidea species in the country to 75. Acknowledgement This work was supported by University Infrastructural Centre “Microscopy of Biological Samples” at the Biotechnical Faculty, University of Ljubljana. Funding This research received no external funding. Contributor(s) Miloš Vittori Corresponding author Miloš Vittori (milos.vittori@bf.uni-lj.si) Leg. Miloš Vittori Country Slovenia Statement of significance This is the first record of Porcellio obsoletus in Slovenia. Locality description Primorska region, Piran, Trubarjeva ulica, 10 m a. s. l. Habitat street with stone pavement Date of observation 2019-06-14 GPS N 45.529361°, E 13.567500° Voucher The specimen is kept in the isopod collection of Miloš Vittori, University of Ljubljana, Biotechnical Faculty, Department of Biology, Večna pot 111, 1000 Ljubljana, Slovenia. Porcellio obsoletus Budde-Lund, 1885, fam. Porcellionidae (animal) 62 Acta Biologica Slovenica, 2024, 67 (2) Figure 1. Porcellio obsoletus, female, col- lected in Piran. A: Habitus. B: Position of glandular pore fields (circled). C: Second antenna with a furrow (arrowheads). 63 Acta Biologica Slovenica, 2024, 67 (2) References Aleffi M., Tacchi R. & Poponessi S. 2020. — New Checklist of the Bryophytes of Italy. Cryptogamie, Bryologie 41 (13): 147-195. https://doi.org/10.5252/cryptogamie- bryologie2020v41a13. Casas, C., Brugués, M., Cros, R. M., Sérgio, C., 2006. Handbook of Mosses of the Iberian Peninsula and the Balearic Islands. Institut Estudis Catalans, Barcelona, Spain. Dollfus, A., 1985. Isopodes terrestres de la région Balkanique (Bosnie, Hercégovine, Serbie et de l'île Corfou). Glasnik zemaljskog muzeja Bosne i Hercegovine, Sarajevo, 7, 525–532. Frey, W., Frahm, J. P., Fischer, E., Lobin, W., Blockeel, T. L. (ed.), 2006. The Liverworts, Mosses and Ferns of Europe. Harley Books, Essex, 512 pp. Godfrey M. 2010. Octodiceras fontanum, Fountain Pocket-moss. In: Atherton I, Bosanquet S, Lewley M (eds.), Mosses and Liverworts of Britain and Ireland, a field guide. British Bryological Society, p 418. Hill, M. O., Preston, C. D., 1998, The geographical relationships of British and Irish bryophytes, Journal of Bryology, 20:1, 127–226, https://doi.org/10.1179/ jbr.1998.20.1.127. Hill, M. O., Preston, C. D., Bosanquet, S. D. S., Roy, D. B., 2007. BRYOATT : Attributes of British and Irish mosses, liverworts and hornworts. Centre for Ecology and Hydrology. Hodgetts, N. G., Lockhart, N., 2020. Checklist and country status of European bryophytes – update 2020. – Irish Wildlife Manuals 123. – Dublin: Department of Culture, Heritage and the Gaeltacht, 224 pp. Hodgetts, N., Cálix, M., Englefield, E., Fettes, N., García Criado, M., Patin, L., Nieto, A., Bergamini, A., Bisang, I., Baisheva, E., Campisi, P., Cogoni, A., Hallingbäck, T., Konstantinova, N., Lockhart, N., Sabovljević, M., Schnyder, N., Schröck, C., Sérgio, C., Sim Sim, M., Vrba, J., Ferreira, C. C., Afonina, O., Blockeel, T., Blom, H., Caspari, S., Gabriel, R., Garcia, C., Garilleti, R., González Mancebo, J., Goldberg, I., Hedenäs, L., Holyoak, D., Hugonnot, V., Huttunen, S., Ignatov, M., Ignatova, E., Infante, M., Juutinen, R., Kiebacher, T., Köckinger, H., Kučera, J., Lönnell, N., Lüth, M., Martins, A., Maslovsky, O., Papp, B., Porley, R., Rothero, G., Söderström, L., Ştefănuţ, S., Syrjänen, K., Untereiner, A., Váňa, J., Vanderpoorten, A., Vellak, K., Aleffi, M., Bates, J., Bell, N., Brugués, M., Cronberg, N., Denyer, J., Duckett, J., During, H. J., Enroth, J., Fedosov, V., Flatberg, K. I., Ganeva, A., Gorski, P., Gunnarsson, U., Hassel, K., Hespanhol, H., Hill, M., Hodd, R., Hylander, K., Ingerpuu, N., Laaka-Lindberg, S., Lara, F., Mazimpaka, V., Mežaka, A., Müller, F., Orgaz, J. D., Patino, J., Pilkington, S., Puche, F., Ros, R. M., Rumsey, F., Segarra-Moragues, J. G., Seneca, A., Stebel, A., Virtanen, R., Weibull, H., Wilbraham, J., Żarnowiec, J., 2019. A miniature world in decline: European Red List of Mosses, Liverworts and Hornworts. IUCN, Brussels. https://doi.org/10.2305/IUCN.CH.2019.ERL.2.en. Holyoak, D., 2013. Notes on identification of species of Bryaceae. (https://www.britishbryologicalsociety.org.uk/wp-content/uploads/2021/03/Notes-on- Bryaceae-Identification-Holyoak-D.T.-2013.pdf). Kaligarič, M., Ivajnšič, D., 2014. Vanishing landscape of the “classic” Karst: Changed landscape identity and projections for the future. Landscape and Urban Planning, 132, 148–158. https://doi.org/10.1016/j.landurbplan.2014.09.004. Martinčič, A., 2024. New checklist and the red list of the mosses (Bryophyta) of Slovenia. Hacquetia, 23 (1), 69–118. https://doi.org/10.2478/hacq-2023-0006. Potočnik, F., 1989. The survey of the fauna of woodlices (Isopoda terrestria) of Yugoslavia. Biološki vestnik, 37, 61–82. Rogenhofer, A.F., 1908. Die zoologische Reise des naturwissenschaftlichen Vereines nach Dalmatien im April 1906. B. Spezieller Teil. Bearbeitung des gesammelten Materials. 9. Isopoda. Mitteilungen des Naturwissenschaftlichen Vereins an der Universitaet Wien, 6, 119–121. Schmalfuss, H., 2003. World catalog of terrestrial isopods (Isopoda: Oniscidea). Stuttgarter Beiträge zur Naturkunde, Serie A, 654. Strouhal, H., 1968. Die Landisopoden der Insel Zypern. Annalen des Naturhistorischen Museums in Wien, 72, 299–387. Vittori, M., Mavrič, B., Pekolj, A., Govedič, M., Zagmajster, M., 2023. A checklist of isopods (Crustacea: Isopoda) in Slovenia. Natura Sloveniae, 25(2), 27–52. https://doi.org/10.14720/ns.25.2.27-52.