ImageAnalStereol2009;28:179-185 OriginalResearchPaper MESH FREE ESTIMATIONOF THESTRUCTUREMODEL INDEX JOACHIMOHSER1,CLAUDIAREDENBACH2ANDKATJASCHLADITZ3 1HochschuleDarmstadt,FachbereichMathematikundNaturwi ssenschaften,Sch¨ offerstraße3,D-64295 Darmstadt, Germany;2TechnischeUniversit¨ atKaiserslautern,FachbereichMat hematik, Erwin-Schr¨ odinger-Straße,D-67653Kaiserslautern,Ger many;3Fraunhofer-Institutf¨ ur Techno-und Wirtschaftsmathematik, AbteilungBildverarbeitung,Fra unhofer-Platz1,D-67663Kaiserslautern,Germany. e-mail: jo@h-da.de,redenbach@mathematik.uni-kl.de,ka tja.schladitz@itwm.fraunhofer.de (AcceptedSeptember30,2009) ABSTRACT Thestructure modelindex(SMI)is a meansof subsumingthe to pologyof a homogeneousrandomclosed set underjustonenumber,similartotheisoperimetricshapefa ctorsusedforcompactsets. Originally,theSMIis defined as a functionof volume fraction, specific surface are a and first derivative of the specific surface area, wherethederivativeisdefinedandcomputedusingasurfacem eshing. The generalised Steinerformulayields howevera derivativeofthespecificsurfaceareathatis– upt o aconstant–thedensityoftheintegralofmean curvature. Consequently, an SMI can be defined without refer ring to a discretisation and it can be estimated from3Dimage data without need to mesh the surface but using the numb erof occurrencesof 2 ×2×2pixel configurations, only. Obviously, it is impossible to comple tely describe a random closed set by one number. In this paper, Boolean models of balls and infinite straight c ylinders serve as cautionary examples pointing out the limitations of the SMI. Nevertheless, shape factors like the SMI can be valuable tools for comparing similarstructures. Thisisillustratedonreal microstruc turesofice,foams,andpaper. Keywords: image analysis, integral of mean curvature, intr insic volume densities, random closed set, shape factor. INTRODUCTION Nowadays, a variety of imaging techniques – first of all computed tomography, but also so-called FIB tomography, electron tomography or atomic force microscopy – are able to produce high quality 3D images of microstructures, increasing the demand for subsequent quantitative analysis. Assuming macroscopic homogeneity, the microstructure can be modelled by a stationary (or macroscopically homogeneous)randomclosedset. In many applications, geometric characteristics of the random closed set have to be estimated from the given image. A very attractive set of such global geometric characteristics are the densities of the intrinsic volumes (or quermassintegralsor Minkowski functionals). In 3D, they are, up to constants, volume fractionVV, surface area density SV, density of the integral of mean curvature MV, and Euler number densityχV. These characteristics can be estimated efficiently from observations in digital binary (black-and-white) images based on discretised Crofton intersection formulae (Ohser et al., 2009; Ohser and Schladitz, 2009).These classical integral geometric formulae allow to compute the intrinsic volumes by calculation of Euler numbers in lower dimensional intersections and subsequent integration over all positions of theintersecting affine subspaces. The Euler numbers in turn can be determined efficiently using the Euler- Poincar´ eformula for all 2 ×2×2pixel configurations and exploiting additivity. The core of the algorithm first outlined by Lang et al.(2001) consists in a convolutionofthebinaryimagewitha2 ×2×2mask, resulting in an 8 bit grey value image, coding the 2×2×2 pixel configurations in the original binary image. Subsequently, the grey value histogram of this image is multiplied by a vector of suitable weights to derive the desired intrinsic volume. It is particularly noteworthy that the size of the grey value histogram does not depend on image size or content. Moreover all measurements are deduced directly from the pixel configurations there is no need to approximate the surfacebyasurfacemesh. A further characteristic for macroscopically homogeneous random sets Ξin the Euclidean space/CA3is thestructuremodelindex (SMI) definedas fSMI=6VVS′ V S2 V, whereS′ Vdenotes the ’first derivative’ of the surface density SV. The SMI was first suggested by Hildebrand and R¨ uegsegger (1997a;b) for evaluating bonestructure. Inordertodefinethederivative S′ V,Hildebrandand R¨ uegseggeruseasurfacemeshing.Roughly,the mesh 179 OHSERJET AL:Meshfree SMI ismovedoutwardsslightlythusdefiningadilationof Ξ byasmallball.Thederivativeisthenapproximatedby the differencequotient. In this paper, S′ Vis definedvia the Steiner formula. As a consequence, the SMI can be expressed in terms of the densities of the intrinsic volumes which in turn allows to estimate the SMI from the grey valuehistogram of the convolvedimage described above. The definition for the SMI without referring to a discretisation as well as the mesh free estimatorarederivedin the sectionbelow. Being a shape factor, the SMI can clearly not capture completely the random closed set under consideration. This is emphasised by the possible ranges for the SMI of Boolean models of balls and infinite straight cylinders. On the other hand, the SMI is helpful for comparing structures that are sufficiently similar like the pore systemsin Greenland firnfromdifferentdepthsorforassessingthedegreeof closednessoftechnicalfoams. DERIVATIONOFTHEMESHFREE ESTIMATOR The class of all compact convex sets (convex bodies) in /CA3is denoted by K. Furthermore, we use thesymbol Rfortheconvexring consistingofallfinite unions of convex bodies. Finally, we introduce the extended convexring Sconsisting of all sets X⊂ /CA3 such that X∩Kis an element of Rfor eachK∈K. Denote by Bra ball with radius rand centred in the origin. Let B(x,r)be a ball with centre xand radius r, thatisB(x,r) =Br+x. TheSteiner formula expresses the volume of the parallelset K⊕Brofaconvexbody Katdistance r>0 asapolynomialofthe intrinsic volumesof KandBr, V(K⊕Br) =3 ∑ k=0r3−kκ3−kVk(K),r≥0,K∈K, (1) (Schneider,1993,p.197).Here κkdenotesthevolume of thek-dimensional unit ball. The intrinsic volumes Vk,k=0,...,3,are defined by (1). They are up to constantsvolume V=V3,surfacearea S=2V2,integral ofmeancurvature M=πV1,andEulernumber χ=V0, andthe integralofmeancurvatureis closelyrelatedto the meanwidth M=2πb. TheSteinerformulacanbeextendedtotheconvex ringR. We use Schneider’s index function j (X,x,y) of a setX∈Ratxwith respect to y, as defined in Schneider and Weil (2008, Section 14.4). Using theEulernumber χ, theindex jis definedas j(X,x,y)=  lim δ↓0lim ε↓0χ(X∩B(x,δ)∩B(y,/bardblx−y/bardbl−ε)), ifx∈X, 0,otherwise for allX∈Randx,y∈ /CA3. It follows from the additivity of the Euler number that the index jis additive in its first argument, too. Now, introducing localparallelsets with multiplicity we definethe local measureρr(X,·)by ρr(X,A) =/integraldisplay/CA3cr(X,A,y)dy,r>0 withcr(X,A,y) =∑ x∈A\{y}j/parenleftbig X∩B(y,r),x,y/parenrightbig , for Borel sets A⊆ /CA3. Here the sum is taken over onlyfinitelymanysummandsdifferentfrom zero.The functional ρrinherits the additivity from the index j. The Steiner formula for the local functional ρrand its extension on the convex ring is given in Schneider (1993, Section 4.4) and Schneider and Weil (2008, Section 14.4). Here we will use the special case A=/CA3, only.Thatis, weconsiderthe functional ρr(X) =ρr(X, /CA3). (2) Let now Ξbe a macroscopically homogeneous randomclosedseton /CA3with realisationsof Ξalmost surely belonging to the extended convex ring S. We assume that Ξis observed through a compact and convex window Wwith nonempty interior. Moreover, assume that Ξfulfils the integrability condition/BX2#(Ξ∩K)<∞foranyconvexbody K∈K,where#X denotestheminimalnumber msuchthattheset Xhasa representation X=K1∪...∪KmwithK1,...,Km∈K. The volume density VV,3ofΞis the expectationof thevolumefraction of ΞinW, VV,3(Ξ) = /BXV3(Ξ∩W) V(W), V(W)>0.Thisdefinitionofthevolumedensitycanbe extendedtothedensitiesoftheotherintrinsicvolumes. The realisations of Ξintersected with aW,a>0 are poly-convex sets. Hence, the intrinsic volumes Vk(Ξ∩aW),k=0,...,3,existandthe intrinsicvolume densitiesV V,kofΞcanbedefinedbythelimits VV,k(Ξ) =lim a→∞ /BXVk(Ξ∩aW) V(aW),k=0,1,2, (cf.Schneider and Weil, 2008). In 3Dthe intrinsic volume densities are (up to multiplicative constants) thevolume density V V=VV,3, thesurface density or specific surface area S V=2VV,2, thedensity of the integralofmeancurvatureM V=πVV,1,andthedensity oftheEulernumber χV=VV,0. 180 ImageAnalStereol2009;28:179-185 The functional ρr(X)as definedin Equation (2) is additive,translationinvariantandlocallyboundedand, hence,its density ρV,r(Ξ) =lim a→∞ /BXρr(Ξ∩aW) V(aW), (3) exists and satisfies a Steiner-type formula, too, as ρr does: ρV,r(Ξ) =3 ∑ k=0r3−kκ3−kVV,k(Ξ),r≥0,(4) (seeSchneiderandWeil,2008,p.428).Nowitfollows that /bracketleftbiggd drρV,r(Ξ)/bracketrightbigg r=0=κ1VV,2(Ξ) =SV and /bracketleftbiggd2 dr2ρV,r(Ξ)/bracketrightbigg r=0=2κ2VV,1(Ξ) =2MV. In this sense we formally write S′ V=2MVand the structure modelindex fSMIis givenby fSMI=12VVMV S2 V, (5) which has a similar structure as a shape factor for convex bodies:Consider the three isoperimetric shape factors for K∈K f1(K) =6√πV(K)/radicalbig S3(K),f2(K) =48π2V(K) M3(K), f3(K) =4πS(K) M2(K). These shape factors are normalised such that f1(Br) =f2(Br) =f3(Br) =1. Deviations from 1 describe various aspects of deviations from ball shape. The shape factor f4(K) =f2(K)/(3f2 3(K)) = 3V(K)M(K)/S2(K)derived from f2andf3is analogousto thestructuremodelindexwhichthuscan be seenasashapefactorforrandomsets. Note however, that S′ Vin this sense is in general not the same as the derivative of SVderived from an infintesimal dilation as defined by Hildebrand and R¨ uegsegger (1997a). Roughly speaking, this is due to the fact that the index function used in the definition of the functional ρcounts signed surface points and thus does not describe a dilation in the case of overlapping grains. Nevertheless, the fSMIas defined here coincides with the orignal SMI by Hildebrand and R¨ uegsegger (1997a) for non-overlapping grains.For an example where the two concepts differ see the following section. For a system Ξof non-overlapping balls of constant radius rwith ball density (mean number of ballspervolumeunit) λwe get fSMI(Ξ) =12VV(Ξ)MV(Ξ) S2 V(Ξ)=12λ4 3πr3λ2π2r λ216π2r4=4. A macroscopically homogeneous system of plate- like structures can only be obtained as a dilated randomhyperplanesystem.Thuswehave MV=0and consequently fSMI=0,too. The third special case considered by Hildebrand and R¨ uegsegger(1997a;b) are sphericalcylinders. Let Ξbe a random system of non-overlapping infinite spherical cylinders of constant radius r, that is a random system of dilated straight lines. This can be achieved e.g. by dilating a system of lines parallel to the z-axis whose feet form a hard core point process inthex-y-plane. LetΞhavelengthdensity(meantotal lengthpervolumeunit) λ. ThenVV(Ξ) =λA=λπr2, SV(Ξ) =λL=λ2πr,andMV(Ξ) =λπ,whereAandL denote the section area and the section circumference ofthecylinders.Thus fSMI(Ξ) =12λπr2λπ (λ2πr)2=3. SMIFORBOOLEANMODELS LetΞbe a homogeneous and isotropic Boolean model in /CA3with typical grain X0and density λ. That is,Ξ=/uniontext∞ i=1(xi+Xi), whereΦ={xi}∞ i=1is a homogeneous Poisson point field, the Xiare i. i. d. likeX0, isotropic, and independent of Φ. LetV,S,b denotetheexpectationsofthevolume,thesurfacearea, and the mean width of the grain X0, respectively, i.e., V= /BXV3(X0),S=2 /BXV2(X0)andb=1 2 /BXV1(X0).Then thevolumefraction VV,thesurfacedensity SV,andthe density of the integral of the mean curvature MVofΞ can be expressed in terms of λ,V,S, andbbyMiles’ formulae: VV(Ξ) =1−e−λV, SV(Ξ) =e−λVλS, MV(Ξ) =e−λV/parenleftbigg 2πλb−π2λ2 32S2/parenrightbigg , (Miles,1976),whichisaspecialcaseofSchneiderand Weil(2008,p.389).By definitionoftheSMIwe get 181 OHSERJET AL:Meshfree SMI fSMI(Ξ) =12(1−e−λV)e−λV/parenleftbigg 2πλb−π2λ2 32S2/parenrightbigg ·e2λV1 λ2S2 =24π(eλV−1)/parenleftBigg b λS2−π 64/parenrightBigg . The dilation of a Boolean model is the same as the Boolean model of the dilated grains ( cf.Chadœf et al., 2008). Thus a derivative of SVcan also be deducedfrom Miles’ formula. As already noted in the previous section, this is not the same as S′ Vdefined above. Consider the special case of the typical grain X0=Brbeingaballofconstantradius r>0.Wehave SV(Ξ) =e−λ4 3πr34πλr2 andthus SV(Ξ)′=e−λ4 3πr3/parenleftbig 8πλr−(4πλr2)2/parenrightbig while 2MV(Ξ) =e−λ4 3πr3/parenleftbig 8πλr−(π2λr2)2/parenrightbig . Nevertheless, all following considerations regarding the significance of fSMIhold analogously for the SMI in the sense of Hildebrand and R¨ uegsegger (1997a), too. In the special case of the typical grain X0=Br being a ball of constant radius r>0 this further simplifiesto fSMI(Ξ) =3(eλ4 3πr3−1)/parenleftbigg1 λπr3−π2 8/parenrightbigg . Thus lim λ→∞fSMI(Ξ) =−∞. On the other hand, fSMI(Ξ)>0 forλ<8/π3r3. Hence, already for one model–theBooleanmodelwithconstantballradius– fSMIcanattain awide rangeofvalues. As a second example we consider now a Boolean cylinder model formed by a Poisson line field with each line dilated by a convex body K∈K. The union of the resulting cylinders is a (generalised) Boolean model. More precisely, let Φ={Li}∞ i=1be a macroscopically homogeneous and isotropic Poisson point field in the space of straight lines in /CA3. Let K1,K2,...be i. i. d. convex bodies. Then the random closed set Ξ=/uniontext∞ i=1(Li⊕Ki)is a Boolean model of infinite straightcylinders. In the special case of the Ki=Bribeing balls, formulae for the densities of the intrinsic volumes in terms of the model parameters were derived by Davy(1978),seealsoOhserandSchladitz(2009).Lettheribe i. i. d. as r0and denote by A=π /BXr2 0the mean cylinder section area and L=2π /BXr0the mean circumference. The density of the Poisson line field is λ.TheMiles’formulae forVV,SVandMVnowbecome VV=1−e−λA SV=λLe−λA MV=/parenleftbigg πλ−π2 32(λL)2/parenrightbigg e−λA. SpiessandSpodarev(2009)provedthesurfacedensity formula for the anisotropic case and general Ki. Hoffmann(2007a;b)derivedfurther generalisationsto inhomogeneousPoissonline fields. Inthecaseofthetypicalgrain Ki=Brbeingaball of constant radius r>0 the Miles’ formulae further simplify to VV=1−e−λπr2 SV=e−λπr22πλr MV=e−λπr2πλ/parenleftbigg 1−π3 8λr2/parenrightbigg , whichgivesforthe SMI fSMI(Ξ) =12(1−e−λπr2)πλ/parenleftBig 1−π3 8λr2/parenrightBig e−λπr2 4π2λ2r2/parenleftbig e−λπr2/parenrightbig2 =3/parenleftBig eλπr2−1/parenrightBig/parenleftbigg1 λπr2−π2 8/parenrightbigg . Thus, lim λ→∞fSMI(Ξ) =−∞whilefSMI(Ξ)>0 for λ<8/π3r2. Given the ranges for the SMI for Boolean models of balls and infinite straight cylinders derived above, there are clearly sets of parameters such that the systems of overlapping balls and the cylinder system havethesameSMI.AsaspecialcaseconsiderBoolean modelsΞbofballswith pointdensity λbandfixedball radiusrbandΞcofcylinderswithlengthdensity λcand crosssectionradius rc.ThenfSMI(Ξb) =fSMI(Ξc) =0 if λb=8 π3r3 bandλc=8 π3r2c. Theseequationshold for instance for λb=1000,rb= 0.064,λc=286.68, andrc=0.03. Realisations of these two models, which are obviously not plate-like, areshownin Fig. 1. 182 ImageAnalStereol2009;28:179-185 (a) (b) Fig.1.VisualisationsofrealisationsofBooleanmodels of spheresandcylinderswith theoreticalvalue f SMI= 0.(a)Booleanmodelofspheres,estimated /hatwidefSMI(Ξb) = −0.022, (b) Boolean model of cylinders, estimated /hatwidefSMI(Ξc) =−0.200. APPLICATION Thesectionaboveshowsthattheinformativevalue offSMIis rather restricted. This holds however for all shape factors. Nevertheless, fSMIis surely a valuable tool for comparing similar microstructures. In the following wewill discussseveralexamples. GREENLAND FIRN(SINTERED SNOW) During the densification of polar firn, significant changes of the microstructure can be observed ( cf. Freitaget al., 2004, and references therein). These include a decrease of porosity with increasing depth butalsochangesofthetopologicalstructureofthepore space from a connected system of pore channels to a systemofisolatedsphericalairbubbles.TheSMImay be usedasameansto characterisethesechanges. As an example we analysed several samples of firn from the firn core B26 which was drilled during the North Greenland traverse of the Alfred Wegener Institute Bremerhaven in 1995. The borehole was located at 77◦15’N, 49◦13’W. Five firn samples taken from different depths within the ice core were imaged using a portable µCT scanner (1074SR SkyScan) inside a cold room at −25◦C. The analysis is based on grey value images consisting of 4003pixels with a pixel size of 40 µm. From these, binary images of the pore system of the firn were obtained by global thresholdingby Freitag etal.(2004). Visualisationsof the firn samplesareshownin Fig.2. (a) 56 m (b) 60 m (c) 69 m (d) 72 m (e) 74m Fig. 2.Visualisations of reconstructed tomographic images of firn samples from five different depths. Samples and imaging: J. Freitag, Alfred- Wegener Institute for Polar and Marine Research, Bremerhaven.Theporesystemis visualised. The estimated values given in Table 1 show an increase of fSMIwith increasing depth. The starting value around 3 indicates a cylindrical structure. The visualisations of the deeper samples already show a numberofisolatedsphericalpores.Therefore,afurther increaseof fSMItowards4canbeexpectedwhengoing deeperwithin thefirn core. Table1.SMIforfirnsampleswith differentporosities. depth[m] porosity[%] /hatwidefSMI 56 15.71 2.954 60 13.16 3.135 69 10.11 3.373 72 9.06 3.410 74 7.83 3.503 183 OHSERJET AL:Meshfree SMI TECHNICALFOAMS Foams are used in an increasing number of application areas including filters, heat exchangers or sound absorbers. They are divided into open-cell foams consisting of a connected system of struts and closed-cell foams whose cells are bounded by membrane-likewalls.However,alsomixedformswith varying proportions of closed walls can be observed. Thedegreeofclosednessofafoamplaysanimportant role for its macroscopic properties. Therefore, easy waysforitscharacterisationarehighlydesirable.Here, weproposetheSMIasameasurefortheclosednessof a foam. (a) (b) (c) (d) Fig. 3.Visualisations of reconstructed tomographic images of four foam samples. (a) Open aluminium foam, sample: m-pore GmbH, imaging: Fraunhofer IZFP, (b) Open nickel-chromium foam, sample: RecematInt.(RCM-NC-2733.10),imaging:RJLMicro & Analytic, (c) Partly open ceramic foam, sample: FOSECO GmbH, imaging: Fraunhofer IZFP, (d) Closed polymer foam, sample and imaging: R. Schlimper,FraunhoferIWM. WeestimatedtheSMIfromtomographicimagesofthe followingfoamsamplesshowingdifferentproportions ofclosedwalls: –an open aluminium foam, 820 ×820×278pixels, pixelsize64 .57µm, –a nickel-chromium foam, used as sound absorber, 800×1600×1600pixels,pixelsize3 .14µm,–a ceramic foam, used for filtering metal melts, 670×670×270pixels,pixelsize70 .88µm, –a closed polymethacrylimide (PMI) foam, used as lightweight core material for sandwich applications, 480 ×480×360 pixels, pixel size 10.21µm. Visualisations of the tomographic images of the foamsamplesareshowninFig.3.TheestimatedSMIs are given in Table 2. None of the estimated SMIs is nearthevalueforanidealcylinderstructure.However, the two open foams have a significantly higher SMI than the (partially) closed samples and the SMI of the closed PMI foam is close to the expected value 0. Moreover, the difference between the strut shape between the aluminium foam (round) and the nickel- chromium foam (trilobal) is clearly reflected by the SMI, too. Table2.SMIforthefoam samples. sample /hatwidefSMI aluminium 2.188 nickel-chromium 1.695 ceramic 0.429 polymer 0.207 PAPER The microstructure of paper determines important properties like tensile strength or filtration properties. Therefore, it has been studied for a long time, however mainly based on 2d images. Here we use a 3Dimage of a recycling paper sample obtained by synchrotron-based phase contrast microtomography at beamline ID22 of the European light source ESRF (employing an effective pixel size of 0.7 µm corresponding to approx. 2 µm spatial resolution, 13 keV monochromatic photon energy). For further details on the experimental setup and the phase retrievalalgorithm applied see Weitkamp et al.(1999) and Paganin et al.(2002), respectively. Most of the cellulose fibres are collapsed and the paper contains variousadditives.This causesthe microstructure to be very irregular. Nevertheless,estimation of the SMI on seven 2603pixel subvolumes yields the values 0.19, 0.37, 0.50, -0.08, 0.2, 0.14, 0.29, still indicating a ratherplatelike structure. Fig.4showsvisualisationsofthetwosampleswith theminimal andmaximalestimatedSMI. 184 ImageAnalStereol2009;28:179-185 (a)/hatwidefSMI=0.50 (b)/hatwidefSMI=−0.08 Fig. 4.Visualisations of two 2603pixel subvolumes of the reconstructed tomographic image of a recycling paper sample. Sample: Papiertechnische Stiftung (PTS), imaging:A.Rack,ESRF,ID-22. DISCUSSION InspiredbyHildebrandandR¨ uegsegger’sstructure model index we define in this paper a shape factor formacroscopicallyhomogeneousrandomclosedsets. This definition can be formulated without use of discretised realisations. Second, we derive a simple estimator for our SMI based on 3Dimage data. Contrary to the originally proposed estimator, ours does not require a surface meshing. Finally we prove that the SMI is of value for the comparison of similar microstructures while suffering from the same shortcomingsasshapefactors forcompactsets. Given the analogy between the SMI and the isoperimetric shape factors pointed out above, other shapefactorscouldbeexplored,too.However,adirect replacement of V, S, and M in the formulae for the isoperimetric shape factors by the respective densities yields scale dependent quantities. In Hildebrand and R¨ uegsegger (1997a), two other shape factors, the structure volume exponent and the structure surface exponent, are suggested. These additionally depend on the thickness of the structure which is assumed to be constant throughout the sample. This assumption, however,is typicallynotmetbyrealmicrostructures. ACKNOWLEDGEMENTS Joachim Ohser and Claudia Redenbach were supported by the FH3-programme of the German Federal Ministry of Education and Research under project grant 1711B06.Katja Schladitz was supported by the German Federal Ministry of Education and Research project 01 SF 0708-III-4a (Fraunhofer- Carnotcooperation).REFERENCES Chadœf J, Bacro J, Th´ ebaudG, Labonne G (2008). Testing the boolean hypothesis in the non-convex case when a boundedgraincanbeassumed.Environmetrics19:123– 36. DavyP(1978).Stereology—AStatisticalViewpoint.Ph.D. thesis,AustralianNationalUniversity,Canberra. Freitag J, Wilhelms F, Kipfstuhl S (2004). Microstructure- dependentdensificationofpolarfirnderivedfromx-ray microtomography.J Glaciol50:243–50. Hildebrand T, R¨ uegsegger P (1997a). A new method for themodelindependentassessmentofthicknessinthree- dimensionalimages.JMicrosc185:67–75. Hildebrand T, R¨ uegsegger P (1997b). Quantification of bone microarchitecture with the structure model index. 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