227 ORIGINAL SCIENTIFIC ARTICLE Developing endurance among children in preschool period 1 Department for Preschool Education, Motopedagogic, Faculty of Education, University of Ljubljana, Ljubljana, Slovenia 2 Faculty of Management, University of Primorska, Koper - Capodistria, Slovenia 3 Faculty of Organizational Sciences, University of Maribor, Kranj, Slovenia 4 Faculty of Sport, University of Ljubljana, Ljubljana, Slovenia Correspondence/ Korespondenca: Jera Gregorc, e: jera. gregorc@pef.uni-lj.si Key words: preschool child; heart rate; types of exercise; cardiovascular system Ključne besede: predšolski otrok; frekvenca srca; vrste vadbe; srčno- žilni sistem Received: 25. 7. 2019 Accepted: 24. 2. 2021 eng slo element en article-lang 10.6016/ZdravVestn.2977 doi 25.7.2019 date-received 24.2.2021 date-accepted Cardiovascular system Srce in obtočila discipline Original scientific article Izvirni znanstveni članek article-type Developing endurance among children in preschool period Razvoj vzdržljivosti predšolskih otrok article-title Developing endurance among children in preschool period Razvoj vzdržljivosti predšolskih otrok alt-title preschool child, heart rate, types of exercise, cardiovascular system predšolski otrok, frekvenca srca, vrste vadbe, srčno-žilni sistem kwd-group The authors declare that there are no conflicts of interest present. Avtorji so izjavili, da ne obstajajo nobeni konkurenčni interesi. conflict year volume first month last month first page last page 2021 90 5 6 227 241 name surname aff email Jera Gregorc 1 jera.gregorc@pef.uni-lj.si name surname aff Maja Meško 2,3 Mateja Videmšek 4 eng slo aff-id Department for Preschool Education, Motopedagogic, Faculty of Education, University of Ljubljana, Ljubljana, Slovenia Oddelek za predšolsko vzgojo, Motopedagogika, Pedagoška fakulteta, Univerza v Ljubljani, Ljubljana, Slovenija 1 Faculty of Management, University of Primorska, Koper - Capodistria, Slovenia Fakulteta za management, Univerza na Primorskem, Koper, Slovenija 2 Faculty of Organizational Sciences, University of Maribor, Kranj, Slovenia Fakulteta za organizacijske vede, Univerza v Mariboru, Kranj, Slovenija 3 Faculty of Sport, University of Ljubljana, Ljubljana, Slovenia Fakulteta za šport, Univerza v Ljubljani, Ljubljana, Slovenija 4 Developing endurance among children in preschool period Razvoj vzdržljivosti predšolskih otrok Jera Gregorc,1 Maja Meško,2,3 Mateja Videmšek4 Abstract Introduction: Prior research generally confirms the importance of endurance exercises in pre- school period and suggests shorter and less intensive exercises. However, little research has been conducted to show and evaluate different types for developing endurance in preschool period. Our research aims to find out which type of exercise is more suitable for developing endurance for a preschool age child compared to an adult, taking into account developmental characteristics. Material and methods: A total of 69 preschool children (40 children aged 3 years and 29 children aged 5 years) were included in the study. We measured their heart rates during two types of en- durance exercises (uninterrupted walking vs “method of game”). We compared the average heart rate and the actual frequency curves. Results: Endurance exercise according to the “method of game” allows the child to adjust the effort more individually compared to uninterrupted walking when both exercises are performed with a group of pre-school children. A comparison of heart rate curves showed that when chil- dren partook in the “ method of game”, they achieved different heart rate values at the same time. A further analysis of heart rate with 5 selected “time stamps” between the two exercises showed that children achieved statistically significant higher heart rates when performing the “method of game” (1’: Z = 2.20; p = 0.028, r = 0.19; 3 ‘: Z = 4.64; p = 0.00, r = 0.39; 5’: Z = 3.23; p = 0.001, r = 0.27; 8’: Z = 3.82 p = 0.00, r = 0.32; 13 ‘: Z = 3.33; p = 0.001, r = 0.28). The comparison of the mean heart rate values over the entire 13-minute time interval between the execution of the “method of game” (M = 143, SD = 14) and uninterrupted walking (M = 132, SD = 9) was statistically significantly higher in favour of the “method of game” (Wilcox test: Z = 5.83, p = 0.00, r = 0.24). The Mann-Whitney-Test showed that 3-year-old children achieved statistically significantly higher av- erage heart rate during the “method of game” (Z = -2.34 p = 0.020, η² = 0.08) as well as during un- interrupted walking (Z = 3.17 p = 0.002, η² = 0.15). No statistically significant differences between boys and girls were confirmed. Conclusions: Based on the results obtained, we believe that endurance exercise according to the “method of game” is more suitable for a developing preschool child than uninterrupted walking. The “method of game” also allows the simultaneous implementation of endurance exercise in heterogeneous groups, since the children adapt to the effort themselves, regardless of age. We believe that other types of endurance exercises should be researched and all age groups of pre- school children should be included in the research. Izvleček Uvod: Pomen vzdržljivostne vadbe v predšolskem obdobju so potrdile že mnoge dosedanje ra- ziskave. Predlagale so predvsem krajšo in manj intenzivno vadbo, manj pa je bilo raziskanega o ustreznosti različnih vrst vadbe za razvoj vzdržljivosti v tem obdobju. Raziskava želi ugotoviti, Slovenian Medical Journal 228 CARDIOVASCULAR SYSTEM Zdrav Vestn | May – June 2021 | Volume 90 | https://doi.org/10.6016/ZdravVestn.2977 1 Introduction Endurance is a functional ability of the organism to perform a movement activity with moderate intensity over a longer period of time (1,2). The efficien- cy of such exercise is limited by the re- spiratory, cardiovascular, thermoregula- tory, and other systems (3) and is closely related to a variety of healthy lifestyle factors (4). According to Degens (5) and his conclusions on the endurance train- ing of children for healthy ageing, aero- bic exercise is still an efficient means of slowing down the loss of muscle mass katera vrsta vadbe za razvoj vzdržljivosti je najprimernejša za predšolskega otroka, upoštevajoč razvojne značilnosti, v primerjavi z odraslo osebo. Material in metode: V raziskavo smo vključili skupaj 69 predšolskih otrok (40 otrok, starih 3 leta, in 29 otrok, starih 5 let). Med izvajanjem dveh vrst vzdržljivostne vadbe (enakomerne neprekinje- ne hoje in vadbe po »metodi igre«) smo merili njihov srčni utrip. Primerjali smo srednje vrednosti srčnega utripa in krivulje dejanskih frekvenc. Rezultati: Vadba vzdržljivosti po »metodi igre« otroku omogoča več individualnega prilagajanja naporu v primerjavi z vadbo neprekinjene hoje, kadar obe vadbi izvajamo s skupino predšolskih otrok. Primerjava krivulj srčnega utripa je pokazala, da pri vadbi po »metodi igre« otroci dosegajo različne vrednosti srčnega utripa ob istem času. Nadaljnja analiza srčnih utripov ob 5 izbranih »časovnih štampiljkah« med obema vadbama je pokazala, da otroci med izvajanjem »metode ig- re« dosegajo statistično značilno višje frekvence srca (1`: Z = 2,20; p = 0,028, r = 0,19; 3`: Z = 4,64; p = 0,00, r = 0,39; 5`: Z = 3,23; p = 0,001, r = 0,27; 8`: Z = 3,82; p = 0,00, r = 0,32; 13`: Z = 3,33; p = 0,001, r = 0,28). Povprečne vrednosti srčnega utripa v celotnem 13-minutnem časovnem in- tervalu med izvajanjem »metode igre« (M = 143, SD = 14) in neprekinjene hoje (M = 132, SD = 9) je bila statistično značilno večja v prid »metodi igre« (Wilcox test: Z = 5,83, p = 0,00, r = 0,24). Mann Whitneyev test je pokazal, da so 3-letni otroci tako med “metodo igre” (Z= -2,34 p = 0,020, η² = 0,08) kot med neprekinjeno hojo (Z= 3,17 p = 0,002, η² = 0,15) dosegli statistično značilne viš- je povprečne vrednosti srčnega utripa. Med spoloma statistično značilnih razlik nismo potrdili. Zaključki: Glede na prej dobljene rezultate menimo, da je vadba vzdržljivosti po »metodi igre« bolj primerna za razvijajočega se predšolskega otroka kot neprekinjena hoja. »Metoda igre« omo- goča tudi hkratno izvajanje vzdržljivostne vadbe v heterogenih oddelkih, saj si obremenitev otro- ci prilagodijo sami, ne glede na starost. Menimo, da bi bilo treba raziskati tudi druge vrste vadbe za razvoj vzdržljivosti, v raziskavo pa vključiti tudi ostale starostne skupine predšolskih otrok. Cite as/Citirajte kot: Gregorc J, Meško M, Videmšek M. Developing endurance among children in preschool period. Zdrav Vestn. 2021;90(5–6):227–41. DOI: https://doi.org/10.6016/ZdravVestn.2977 Copyright (c) 2021 Slovenian Medical Journal. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. and maintaining its function, thus im- proving the quality of life. Therefore, dy- namic aerobic endurance is the one that should be promoted in the preschool period (2,6). However, the way in which such training is carried out differs be- tween adults and children. It is a long- term, moderately intensive cyclical exer- cise (e.g. running, walking, cycling, etc.) that adults perform continuously, while children perform it more intensively and with more frequent breaks (2,6). This is due to a more specific function of the 229 ORIGINAL SCIENTIFIC ARTICLE Developing endurance among children in preschool period cardiovascular, respiratory, neuromus- cular, and thermoregulatory systems of children. Antipuesto (7) and Kliegman, Stanton, St. Geme, and Schor (8) analysed the differences between the cardiovascular and respiratory systems of children and adults. In their opinion, the tongue is one of the limiting factors in the respiratory system of a preschool child. Airflow is lower in children than in adults because of the different proportions between the airways and the tongue (7). For this rea- son, breathing through the nose rather than through the mouth is more import- ant for children (8). Samuels and Wieteska (9) give guide values for differences in minute venti- lation. It is known that minute ventila- tion is the product of the breathing rate in one minute and a breathing volume. Samuels and Wieteska (9) give mean values for the respiratory rate of chil- dren and adults. The respiratory rate per minute of a 3-year-old child is 25 to 30 breaths per minute, while in 5-year-old children it is slowed to a range between 20 and 30 breaths per minute, the respi- ratory rate of adults at rest is about 12 to 16 breaths per minute, which means that adults have more opportunities to increase the respiratory rate per min- ute compared to children (9). They ar- gue that greater minute ventilation at rest in children is also due to greater metabolic consumption. However, they believe that children still have an appar- ently elastic chest. Therefore, increased breathing effort results in an obvious thoracic retraction, which reduces respi- ratory efficiency. All these facts point to the importance of more frequent breaks for children compared to adults. Dotan, Mitchell, Cohen, Klentrou, Gabriel, and Falk (10) analysed differ- ent studies on the types of muscle fibres. They concluded that children have more type 1 muscle fibres. This makes them tired more quickly. However, their mus- cle fibres also regenerate faster, which is an additional reason for more frequent breaks. Krost, Mistovich and Limmer (11) explained differences in heart function in adults and children. The heart of an adult responds to increased stress by increasing both heart rate and stroke volume, while the heart of a child can only respond to increased stress by in- creasing the number of heartbeats. This is the reason why a child has less ability to adapt compared to an adult. Fleming, Thompson, Stevens, Heneghan, Plueddemann, Maconochie, Tarassenko and Mant (12) and Agrawal (13) ana- lysed the differences in heart rate be- tween adults and children at rest and at the strain of 80%. They concluded that the heart rate of children who were 3 or 4 years old was 3 to 4 beats higher than that of children who were 5 or 6 years old. Different stress tests in laboratories are used to measure how an adult’s heart responds to a strain. There are not ma- ny stress tests for healthy children. Even paediatricians conclude that it is neces- sary to replace the stress testing with the measurement of heart rate in preschool children (8). The strain level is measured outdoors, usually with heart rate mon- itors (14), especially because they are more cost-efficient, while stress tests in a laboratory are also followed by mea- suring the oxygen saturation of blood, ECG, blood pressure, breathing (max- imum oxygen uptake), etc. Regardless of the age, exercise intensity is usually defined by the percentage of maximum oxygen uptake (% VO2max), maximum heart rate (% FSmax), and reserve heart rate (% HRres), but also as metabolic 230 CARDIOVASCULAR SYSTEM Zdrav Vestn | May – June 2021 | Volume 90 | https://doi.org/10.6016/ZdravVestn.2977 equivalent (1 MET) (14). The same au- thors (14) suggested that the measure- ment of HRmax should be performed as an alternative to establish the submaxi- mal intensity, as there is a linear relation between VO2max and HRmax. However, there are not many comparisons between maximum oxygen uptake and heart rate in the preschool period (15). To ensure an adequate level of effort, we must first know at least the individu- al HRmax, VO2max, and two ventilation thresholds (16). The ventilation thresh- olds are most accurately determined by laboratory stress tests. The majority of the parameters can be obtained only in laboratories, and are not common in general practice (too expensive and too complicated) (17). Indirectly, however, some of the parameters can be calcu- lated from a series of more or less reli- able tests performed in the field, such as “UKK WALK TEST”, “CONCONI TEST”, “20mSRT-PREFIT”, etc., with simultaneous measurement of HR (18,19). By drawing a curve from the data obtained (x-axis time, y-axis HR) the two ventilation thresholds can be figured out and the intensity of exercis- es can be divided into 3 zones (16). The endurance exercise is then performed with a combination of different “meth- ods” (uninterrupted method, interval method, and Fartlek) at different levels of intensity oz. zones (20). Uninterrupted exercise for adults is usually performed individually. Exercises are most often performed under the first ventilation threshold (app. 75-80% of HRmax). However, it can be performed at different inten- sities. The exercise is mainly charac- terized by cyclical, repetitive move- ments, which usually last at least one hour (20). During the performance of such exercise, an attempt to maintain approximately the same effort is made. We must ensure that the development of endurance in the preschool period is within the 80% HRmax range (21). In general practice, however, it is not pos- sible to ensure individual uninterrupted movement, which is why the educators adjust the pace to the weakest during hikes, walks, mountaineering, and short runs (6). However, such an effort is not sufficiently intensive for the develop- ment of other children’s endurance (17). A “method of game” can be suitable for individual exercise with a group of children during developing endurance. This exercise is described under the con- cept “Game - movement - development” (male). The comparison of heart rate curves conducted on a small sample (6) indicates that the heart of a 3- or 6-year old child responds in a similar way as the heart of an adult responds to a Fartlek (6). Fartlek represents a combination of low- and high-intensity exercise, as it can include both brisk walking and sprinting in one exercise and is an effective means of developing basic endurance (22). Because of all the content, length, and intensity combinations, it is often used as a universal tool for the development of endurance (20). It is essential that the range and length are not precisely deter- mined and the intensity is determined spontaneously (22). The “method of game” differs from the Fartlek mainly in performance. The “method of game” is usually performed with a whole group of children while individual exercise is typ- ical during Fartlek. The “method of game” is a way of learning in the preschool period, and it is based on the concept of Game- Movement-Development. According to this “method”, a child develops the mo- tor skill that the educator wants to devel- op in a child, which also implies obeying 231 ORIGINAL SCIENTIFIC ARTICLE Developing endurance among children in preschool period the rules of the game. The child reacts in an unobtrusive way to the rules and environment, and the educator at the same time can take into account the in- dividuality of each child. In the “method of game”, the educator must be includ- ed in the game as a person in the game or must take over the role of a so-called environment. This enables him or her to change the level of difficulty or to adapt it to children at any time. In addition, the beforehand prepared environment facilitates children to search for creative motoric solutions by themselves within the game, to set pauses, their duration, frequency, and intensity. By correctly devised game rules, the educator boosts motivation, which makes children re- peat the running to succeed in the game. For example, the child’s goal is to suc- ceed in the game (to rescue the princess from the dragon’s castle), and the educa- tor’s goal is that the child scuds or walks the distance as many times as possible, climb the hill or stairs to higher floors (23). The main difference between uninter- rupted walking and exercise according to the “method of game” lies in the indi- vidual determination of the effort. With uninterrupted walking, the pace is usu- ally determined by the educator, where- by the weakest limb is usually taken into account. With the “method of game” the pace of walking as well as the length is determined by the children themselves, similar to Fartlek. Due to anatomical and pathophysi- ological differences between adults and children, as well as due to differences in physical fitness between children in the same group and the need to develop en- durance within the entire group of chil- dren at the same time, it seems that un- interrupted walking may be less suitable for developing endurance in preschool. Considering that children themselves determine the intensity during exercise according to the “method of game”, we want to investigate whether they achieve higher or lower effort than with uninter- rupted walking, where the intensity is usually determined by the educator. 2 Material and methods 2.1 Participants The study included 69 preschool chil- dren (40 3-year-old and 29 5-year-old children; 44.9% of girls and 55.1% of boys) from four different kindergartens in the central Slovenian region. All four kindergartens had approximately the same outdoor conditions (a small grassy hill with a lawn of at least 20 by 20 metres around it) to implement the “method of game” and 1 km of the straight sidewalk (mostly macadam) to perform unin- terrupted walking. Both exercises were carried out as part of the regular pro- gramme without any significant changes to their activities. The Commission for Ethical Issues in the field of sport at the Faculty of Sport, University of Ljubljana, approves the conduct of research (No. 1466, dated 26 September 2019). 2.2 Instruments During the research, children wore heart rate sensors (H7, Polar), connect- ed to fitness monitor tracker (Polar loop, Polar Electro Oy, Kempele, Finland). The heart rate (HR) was measured in 5-s periods. After finishing the individual exercise, we downloaded the data from the heart rate monitors to the comput- er using the Polar FlowSync programme and classified the data according to re- search codes. 232 CARDIOVASCULAR SYSTEM Zdrav Vestn | May – June 2021 | Volume 90 | https://doi.org/10.6016/ZdravVestn.2977 2.3 Procedures Children’s parents or caregivers were informed about the goals of the research. They completed a short survey question- naire about the child’s health status and signed consent for testing. To ensure an- onymity, a research code was assigned to each child. The code was linked to the heart rate monitor. The research was conducted in compliance with personal data protection. The variable sample includes the heart rates of all children measured during two different types of endurance exercises: (1) the uninterrupted walk- ing, which consisted of a 1 km of walk- ing in 13 minutes, and (2) the “method of game” – regarding this method, only 13 minutes of the main part of the hour were included in the research. With the first exercise (the uninterrupted walk- ing), the strain was determined by time and distance whilst with the second ex- ercise (the “method of game”), it was de- fined by time and the rules of game. All children were monitored during both exercise models. They partook scheduled exercise sessions on two dif- ferent Wednesdays of May 2016 at 10 AM; the first week they partook in un- interrupted walking; the following week they partook in “method of game”. The activities with younger and older chil- dren were performed separately. Both exercises were performed by qualified students of Preschool education (UL, PeF), in the presence of the preschool educator in the kindergarten and under the supervision of the researcher. To avoid any risk, all children had to wear sports shoes and sports clothes. The children knew both exercises, as they were often performed in all kin- dergartens. To avoid injuries when im- plementing the “method of game”, we have chosen the grassy lawn in front of the kindergartens and macadam side- walk when implementing uninterrupted walking. (1) The uninterrupted walking The uninterrupted walking applied through a moderately intensive walk on a distance of 1 km for 13 minutes. It was indeed a modified walking test on 2 km “UKK WALK TEST” (18). We wanted to create artificial conditions by shorten- ing the distance and increasing the time. Such conditions should be appropriate for preschoolers proportionally to the differences between metameres of chil- dren and adults. The test was modified and it was conducted through walks and excursions. The student made sure that the children walked in a group at an av- erage speed of 4.6 km/h and all did 1 km in 13 minutes at the same time. (2) The “method of game” The same content for all children was chosen when performing the exer- cise according to the “method of game”, namely, “The delivery of postal items”. The student was a “postman”. The chil- dren were “letter-carriers”. The postman always had enough different envelopes with different degrees of urgency. The letters had to be delivered to the right addresses. The titles were distributed in the outdoor playground of each kin- dergarten. The children could choose to whom they brought which letter, how often they chose a letter, how quickly they wanted to pick up a new letter. So they could determine the intensity of the exercise, the dosage, and the type of movement. 2.4 Statistical analysis To analyse the differences in heart rate (HR) responses to these two types of exercises, we transformed the data into 233 ORIGINAL SCIENTIFIC ARTICLE Developing endurance among children in preschool period heart rate curves and compared them at the same points in time. The heart rate was measured in 5 seconds intervals during both exercises. From a sample of 69 children we ran- domly selected 10 3-year old children and 10 5-year old children. We formed 10 pairs. Each pair had one 3-year-old and one 5-year-old child. We plotted 40 graphs. The first 10 graphs show the response of the hearts of two children (pair) during uninterrupted walking at the same time (simultaneously). The next 10 graphs show the response of the hearts of the same pair of children during the “method of game”. Simultaneous repre- sentations of the response of each child’s heart to both exercises are shown in the last 20 diagrams. (Due to limited article space, only 3 out of all will be presented for illustration in the Results.) For statistical analyses, we used sta- tistical package SPSS 20 (SPSS Inc., Chicago, IL). We obtained 312 (2 x 156) heart rate values for each child during both exercises. To determine the individuality of the effort during exercises, we randomly se- lected “time stamps”, namely at the 1st, 3rd, 5th, 8th and 13th minute. We made a printout of all heart rates at the selected “time stamp” and calculate descriptive statistics (HR means ± SD). Normality assumptions for all data were tested us- ing Shapiro Wilk (S-W) Test. Since the distribution was not normal, we run nonparametric tests. Wilcoxon Rank Sum Test was used to determine wheth- er the difference in heart rates was sta- tistically significant at the same “time stamp”. A p-value of 0.05 or lower was considered statistically significant. Effect size was tested with r square; 0.10 < 0.3 (small effect), 0.30 < 0.5 (moderate ef- fect) and >= 0.5 (large effect). For all subsequent statistical treat- ments, we took all data from both ex- ercises. We calculated the average heart rate for each child individually, sepa- rately according to the type of exercise. The data were summarised using routine descriptive statistics (HR means ± SD). Normality assumptions for all data were tested using Shapiro Wilk (S-W) test. Since the distribution was not normal, we run nonparametric tests. To compare the differences in aver- age heart rate during the two exercises, we used Wilcoxon Rank Sum. A p-value of 0.05 or lower was considered statis- tically significant. Effect size was tested with r square; 0.10 < 0.3 (small effect), 0.30 < 0.5 (moderate effect) and >= 0.5 (large effect). To compare the differences (separately between boys and girls and separately between 3- and 5-year old children) in average heart rate during the two exercises, we used the Mann- Whitney Test. A p-value of 0.05 or lower was considered statistically significant. To measure the effect size, Eta square (η²) was calculated. 3 Results The results are presented in figures (graphs) and tables. First of all, due to limited article space, we show only three (3) graphs with comparisons of the heart reactions for a randomly selected pair of children (one pair). The first graph (Figure 1) shows the difference between the heart curves during uninterrupted walking, the second (Figure 2) during the “method of game”, and the third (Figure 3) shows the difference in heart rate response to the two types of exercise for the senior child from a pair. Figure 1 shows the differences in heart response during the uninterrupt- ed walking between two preschoolers. 234 CARDIOVASCULAR SYSTEM Zdrav Vestn | May – June 2021 | Volume 90 | https://doi.org/10.6016/ZdravVestn.2977 Figure 2 refers to the same couple of two preschoolers as with the uninter- rupted walking (Figure 1). Again, we have a sample which indicates that also with this type of exercise the younger child performed a more intensive move- ment activity. It shows that the young- er child did not stop at a higher level of intensity or took the necessary rest at once. After analysing all the other 9 graphs plotted from HR, obtained during “method of game”, we came to the con- clusion that a child can adjust its own intensity of movement at any time and therefore the individual stress is guar- anteed by the feeling of tension and the need for rest. The third graph (Figure 3) shows the differences in the reaction of the heart of a 5-year-old child (from the previously selected and represented couple) to two different types of exercises. According to the analysis of all 20 individual graphs (for illustration, only one of them is presented in Figure 3), we concluded that the curves obtained by measuring the children during un- interrupted walking is more linear than Figure 2: The presentation of heart response during the “method of game” from randomly chosen pairs (one 3- and one 5-year-old preschooler). 220 200 180 160 140 120 100 80 60 40 20 0 m g _ 1 m g 1 0 m g _ 1 9 m g _ 2 8 m g _ 3 7 m g _ 4 6 m g _ 5 5 m g _ 6 4 m g _ 7 3 m g _ 8 2 m g _ 9 1 m g _ 1 0 0 m g _ 1 0 9 m g _ 1 1 8 m g _ 1 2 7 m g _ 1 3 6 m g _ 1 4 5 m g _ 1 5 4 m g _ 1 6 3 m g _ 1 7 2 m g _ 1 8 1 m g _ 1 9 0 m g _ 1 9 9 m g _ 2 0 8 m g _ 2 1 7 m g _ 2 2 6 m g _ 2 3 5 m g _ 2 4 4 m g _ 2 5 4 Time period in 5s intervals H e a rt r a te 3 years 5 years According to this chart, the heart rate of the younger child varied more fre- quently than the heart rate of the older child(ren). However, also in the older child, the frequency was not steady, as we could expect in adults. Based on a more accurate analysis of these two curves, the average value of the older child was for 3 strokes per minute lower than the one of the younger child. The older child’s chart has only one emphasised and more du- rable higher heart rate, while the chart of the younger child has a more frequent and shorter higher heart rate. Figure 1: The presentation of heart response during the uninterrupted walking from a randomly chosen pair (one 3- and one 5-year-old preschooler). 200 180 160 140 120 100 80 60 40 20 0 w _ k m _ 5 w _ k m _ 4 5 w _ k m _ 8 5 w _ k m _ 1 2 5 w _ k m _ 1 6 5 w _ k m _ 2 0 5 w _ k m _ 2 4 5 w _ k m _ 2 8 5 w _ k m _ 3 2 5 w _ k m _ 3 6 5 w _ k m _ 4 0 5 w _ k m _ 4 4 5 w _ k m _ 4 8 5 w _ k m _ 5 2 5 w _ k m _ 5 6 5 w _ k m _ 6 0 5 w _ k m _ 6 4 5 w _ k m _ 6 8 5 w _ k m _ 7 2 5 w _ k m _ 7 6 5 w _ k m _ 8 0 5 w _ k m _ 8 4 5 w _ k m _ 8 8 5 w _ k m _ 9 2 5 w _ k m _ 9 6 5 w _ k m _ 1 0 0 5 w _ k m _ 1 0 4 5 w _ k m _ 1 0 8 5 w _ k m _ 1 1 2 5 w _ k m _ 1 1 6 5 w _ k m _ 1 2 0 5 w _ k m _ 1 2 4 5 Time period in 5s intervals H e a rt r a te 3 years 5 years 235 ORIGINAL SCIENTIFIC ARTICLE Developing endurance among children in preschool period Figure 2 refers to the same couple of two preschoolers as with the uninter- rupted walking (Figure 1). Again, we have a sample which indicates that also with this type of exercise the younger child performed a more intensive move- ment activity. It shows that the young- er child did not stop at a higher level of intensity or took the necessary rest at once. After analysing all the other 9 graphs plotted from HR, obtained during “method of game”, we came to the con- clusion that a child can adjust its own intensity of movement at any time and therefore the individual stress is guar- anteed by the feeling of tension and the need for rest. The third graph (Figure 3) shows the differences in the reaction of the heart of a 5-year-old child (from the previously selected and represented couple) to two different types of exercises. According to the analysis of all 20 individual graphs (for illustration, only one of them is presented in Figure 3), we concluded that the curves obtained by measuring the children during un- interrupted walking is more linear than Figure 2: The presentation of heart response during the “method of game” from randomly chosen pairs (one 3- and one 5-year-old preschooler). 220 200 180 160 140 120 100 80 60 40 20 0 m g _ 1 m g 1 0 m g _ 1 9 m g _ 2 8 m g _ 3 7 m g _ 4 6 m g _ 5 5 m g _ 6 4 m g _ 7 3 m g _ 8 2 m g _ 9 1 m g _ 1 0 0 m g _ 1 0 9 m g _ 1 1 8 m g _ 1 2 7 m g _ 1 3 6 m g _ 1 4 5 m g _ 1 5 4 m g _ 1 6 3 m g _ 1 7 2 m g _ 1 8 1 m g _ 1 9 0 m g _ 1 9 9 m g _ 2 0 8 m g _ 2 1 7 m g _ 2 2 6 m g _ 2 3 5 m g _ 2 4 4 m g _ 2 5 4 Time period in 5s intervals H e a rt r a te 3 years 5 years those obtained during the “method of game”. An increased heart rate during the “method of game” indicates that children were moving more often and with more intensity than during unin- terrupted walking. However, they also rested more often during the “method of game” than during uninterrupted walking. To support the above claims from the graphs that the “method of game” al- lowed the child to individualise the ex- ercise more than uninterrupted walking (more breaks, more different intensities at the same time) and still develop me- dium to high endurance, we have creat- ed five “time stamps” (at 1, 3, 5, 8 and 13 minutes) and calculated average HR and SD at all selected points in time (Table 1). When we observed the differences in the average of HR (but especially in SD) between all children at the same time point, we found out that heart rates during the “method of game” dif- fered more than during the uninterrupt- ed walking (at 1` SDmg-SDuw = 21-10; 3`SDmg-SDuw = 23-9; 5` SDmg-SDuw = 30-18; at 8` SDmg-SDuw= 26-10; at 5` SDmg-SDuw = 24-8). Figure 3: The comparison of heart response with the uninterrupted walking and “method of game” in the same 5-year old child. 200 180 160 140 120 100 80 60 40 20 0 5 5 0 9 5 1 4 5 1 8 5 2 3 0 2 7 5 3 2 0 3 6 5 4 1 0 4 5 5 5 0 0 5 4 5 5 9 0 6 3 5 6 8 0 7 2 5 7 7 0 8 1 5 8 6 0 9 0 5 9 5 0 9 9 5 1 0 4 0 1 0 8 5 1 1 3 0 1 1 7 5 1 2 2 0 1 2 6 5 Time period in 5s intervals H e a rt r a te Method of play Unint. method 236 CARDIOVASCULAR SYSTEM Zdrav Vestn | May – June 2021 | Volume 90 | https://doi.org/10.6016/ZdravVestn.2977 Wilcoxon Rank Sum test showed that differences at all points in time were statistically significant (at 1`: Z = 2.20; p  =  0.028, r = 0.19; 3`: Z = 4.64; p = 0.00, r = 0.39; 5`: Z = 3.23; p = 0.001, r = 0.27; 8`: Z = 3.82; p = 0.00, r = 0.32; 13`: Z = 3.33; p = 0.001, r = 0.28). Table 2 shows that the average heart rate of all participants during the perfor- mance of the “method of game” was 143, with a standard deviation of 14. The av- erage heart rate of all participants during uninterrupted walking was 132, with a Table 1: Average HR values for all children at selected “time stamps” (i.e. at the 1st, 3rd, 5th, 8th and 13th minute). Legend: N – number of replies; Min – minimum value; Max – maximum value; Z – value of Wilcoxon Rank Sum; sig. - statistical significance – a p-value of 0.05 or lower was considered statistically significant. Stamp time Exercise N Mean value Std. deviation Min Max Wilcoxon Rank Sum Z sig. 1st min “method of game” 69 132 21 102 179 2.20 0.028 uninterrupted walking 69 125 10 102 158 3rd min “method of game” 69 135 23 107 181 4.64 0.000 uninterrupted walking 69 119 9 105 149 5th min “method of game” 69 140 30 95 195 3.23 0.001 uninterrupted walking 69 125 18 104 169 8th min “method of game” 69 145 26 110 193 3.82 0.000 uninterrupted walking 69 129 11 111 143 13th min “method of game” 69 145 26 105 186 3.33 0.001 uninterrupted walking 69 133 8 122 153 standard deviation of 5. This illustrates the fact that uninterrupted walking is less intense and results in a smaller range of heart rate. This is further illustrated by the minimum and maximum heart rate. The difference between the minimum and maximum heart rate during the “method of game” (HR max: 178 – HR min: 110) was 68 beats, while during the uninterrupted walking it was 28 beats (HR max: 146 – HR min: 118). The distribution was not normal, so we used the Wilcoxon Rank Sum test to Table 2: Average heart rate during 13 minutes of uninterrupted walking and “method of game”. Legend: N – number of replies; Min – minimum value; Max – maximum value; Z – value of Wilcoxon Rank Sum; sig. - statistical significance – a p-value of 0.05 or lower was considered statistically significant. N Mean value Std. deviation Min Max Wilcoxon Rank Sum Z sig. “Method of game” 69 143 14 110 178 -5.83 0.000 Uninterrupted walking 69 132 5 118 146 237 ORIGINAL SCIENTIFIC ARTICLE Developing endurance among children in preschool period identify statistical differences. Wilcoxon Rank Sum test showed that differences were statistically significant (Z = -5.83; p  = 0.001, r = 0.24). On average, the children experienced a higher intensity when endurance was implemented ac- cording to the “method of game”. Given the differences between the minimum and maximum values, the effort would not be constant. Table 3 shows heart rate average val- ues of boys and girls obtained during uninterrupted walking and the “method of game”. We applied the Mann Whitney test to verify whether the differences in the average heart rate between boys and girls regarding the type of exercise were statistically different. From the results of Mann Whitney test, we can notice that there is no significant difference between girls and boys neither during uninter- rupted walking nor during the “method of game”. We analysed differences between age groups in the same way as we analysed differences between boys and girls. Table 3: Average heart rate for boys and girls during uninterrupted walking and “method of game”. Legend: N – number of replies; Min – minimum value; Max – maximum value; sig. - statistical significance – a p-value of 0.05 or lower was considered statistically significant. N Mean value Std. deviation Min Max Mann- Whitney test “Method of game” Boys 38 143 14 110 178 0.491Girls 31 142 14 110 178 Total 69 143 14 110 178 Uninterrupted walking Boys 38 133 6 118 146 0.132Girls 31 130 4 123 136 Total 69 132 5 118 146 Table 4: Average heart rate for 3- and 5-year old preschoolers during uninterrupted walking and “method of game”. Legend: N – number of replies; Min – minimum value; Max – maximum value; sig. - statistical significance – a p-value of 0.05 or lower was considered statistically significant. N Mean value Std. deviation Min Max Mann- Whitney test “Method of game” 3-year 40 146 17 110 178 0.0205-year 29 139 7 128 150 Total 69 143 14 110 178 Uninterrupted walking 3-year 40 133 3 125 136 0.0025-year 29 130 7 118 146 Total 69 132 5 118 146 238 CARDIOVASCULAR SYSTEM Zdrav Vestn | May – June 2021 | Volume 90 | https://doi.org/10.6016/ZdravVestn.2977 Table 4 shows that 3-year-old chil- dren had higher heart rate average val- ues during both types of exercises in comparison to 5-year-old children. The difference during the “method of game” was 7 strokes per minute, where- as, during the uninterrupted walking, this difference was lower and equal to 2 strokes per minute. The Mann Whitney Test indicates statistical significant dif- ferences in average of heart rate be- tween age during the “method of game” (Z = -2,34 p = 0.020, η² = 0.08) and un- interrupted walking (Z = 3.17 p = 0.002, η² = 0.15). 4 Discussion The study examined the reactions of the heart of preschool children to two different types of endurance exercise (i.e. uninterrupted walking vs “method of game”). Endurance itself is a func- tional ability of an organism to perform a movement activity with moderate in- tensity for a longer time (1,2). The reac- tion of the body to given stress (strain) is called effort (22). Usually, the strain is determined by the speed of the move- ment, length , frequency, type of exer- cise, etc. In our study, the strain for the first exercise (uninterrupted walking) was 1 km walking for 13 minutes. For the second exercise (“method of game”) the reverse reaction (effort vs strain) was desired to achieve. The child should determine the strain themselves during the implementation of the “method of game” according to the effort they expe- rienced at that moment. The discussion is divided into three paragraphs according to the main re- search question which one of the two exercises is more suitable for developing endurance in preschoolers. In the first part, the connection between the graphs and the Table 1 with the individuality of the exercise is shown. In the second paragraph, the results of Table 2 are re- lated to the exercise intensity. The third paragraph, based on the results of Tables 3 and 4, points out the universality of the “method of game” and its relation to Fartlek. Measuring adult HR, the steady- state is established by performing a cyclic movement at a constant speed on the aerobic threshold (24). By com- paring our results obtained by measur- ing HR during uninterrupted walking for 1 km at 4.6 km/h speed, all chil- dren failed to reach steady-state. The heart rates of 3-year old children varied more frequently compared to 5-year old children. However, also all graphs of 5-year old children were not steady, as we could expect in adults. According to the first part of our results (Figures 1-3 and Table 1), where we wanted to explore the possibilities of adapting the intensity to the individual, we noticed that children at the same time (“time stamp”) associated more variegated HR during the “method of game” than during uninterrupted walking. We con- cluded that just by shortening the time of exercise and decreasing the distance of walking, we only shorten the time of developing aerobic activity. The reasons for the inability to perform exercises in a steady-state are sought in incompletely mastered walking as well as in the an- atomical and physiological differences between children and adults. Dotan, Mitchell, Cohen, Klentrou, Gabriel and Falk (10) concluded that children have more muscle fibres of type 1. Because of that, they get tired more quickly. However, their muscle fibres also rein- vigorate faster, which is an additional reason for more frequent pauses. The analysis of heart stroke curves measured 239 ORIGINAL SCIENTIFIC ARTICLE Developing endurance among children in preschool period during the “method of game” might in- dicate that this is exactly the reason for more frequent rests and quick increase of heart rate after rest. Krost, Mistovich and Limmer (11) also analysed the dif- ferences in cardiac function between adults and children. According to them, the heart of an adult responds to an in- creased strain by increasing both the heart rate and stroke volume, while a child’s heart can respond to the in- creased strain only by increasing the number of heart strokes. This might be the reason for considerable fluctuations in heart rate. In the second paragraph, the inten- sity between exercises regarding strain determiner is discussed. According to the results from Table 2, children expe- rienced a higher intensity when endur- ance was implemented according to the “method of game” in comparison to un- interrupted walking. Preschool teachers need to ensure safety when organizing uninterrupted walking. All children need to walk in a group with the same pace (17). Since they have different lev- els of walking ability (6), different phys- ical conditions (17), and differently de- veloped cardiovascular and respiratory systems (10-14), the preschool teacher cannot take all these individual differ- ences into account. As a rule, they select the child who has the least developed endurance and does not give others the appropriate intensity. In the third paragraph, the results in Tables 3 and 4 are compared with Fartlek, which is considered as a uni- versal method of endurance develop- ment. Aerobic endurance development exercises are more and more sophisti- cated and include strains for both aero- bic and anaerobic metabolism. Roughly, they can be divided into three methods, namely: the uninterrupted exercise, interval exercise and Fartlek (which is the game of agility). Stöggl and Sperlich (24) concluded that the combination of all methods was important for aer- obic endurance development in adult sportspersons. Gregorc and Humar (6) compared a pre-schooler’s heart re- sponse with the »method of game« with adults’ heart responses with all three endurance development methods and concluded that a child’s heart response was most similar to adult’s heart re- sponse when performing the Fartlek ex- ercise. In our study, the 3-year-old chil- dren achieved higher average heart rate during both exercises in comparison to 5-year old children; during the “method of game” (Z = -2.34 p = 0.020, η² = 0.08) as well as during uninterrupted walking “(Z = 3.17 p = 0.002, η² = 0.15) (Table 4). However, a small variance was clarified. However, we believe that it would make sense to carry out uninterrupted walk- ing with both younger and older chil- dren in a different way. The preschool teacher should guide the children to safe walking paths on which they should be allowed short runs, breaks, alternating fast and slow walking. Based on the re- sults of Table 3 (no statistically signifi- cant differences between boys and girls were confirmed), it makes no sense to divide the children by gender. This study has four main limitations: • the first is a relatively limited number of participants; • the second is short-term observation, with no answer to long-term endur- ance; • the third is only two different types of exercises; • and the fourth is measuring intensity only through heart rates. Further prospective studies may an- swer some of these questions. 240 CARDIOVASCULAR SYSTEM Zdrav Vestn | May – June 2021 | Volume 90 | https://doi.org/10.6016/ZdravVestn.2977 5 Conclusions The intention of this research was to explore the adequacy of using different methods for developing endurance for preschool children than for adults. 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