Dostavni sistemi nanometrskih velikosti za vnos proteinov in genov
Nanosized drug delivery systems for proteins and genes

slovenian

2005

volume 44, issue 4

pharmacy    drug    therapy 

Društvo Medicinski razgledi
(Obvezni izvod spletne publikacije)

In the article we review most of the systems and approaches for delivery of protein drugs and therapeutic genes into the body. Issues in drug delivery arebecoming more important as more potent and specific drugs become available with the knowledge about diseases available from the human genome project. To achieve optimal therapeutic efficacy, drug delivery systems should be customized very innovative. Novel systems are often nanosized, prepared by specific excipients, and they have multifaceted advantages in drug delivery. These systems in general can be used to provide targeted (cellularžtissue) delivery of drugs, to improve oral bioavailability, to sustain drugžgene effect at target place, and to improve the stability of therapeutics agents against enzymatic degradation, especially of protein, peptide and nucleic aciddrugs. Vectors for gene delivery could be divided into two major groupsČ viral and nonviral. Due to several limitations of viral vectors more attentions attract nonviral vectors, which consist of a carrier with cationic character able to associate with negatively charged DNA molecule. Formation ofthe complex protects DNA against denaturation and enables its entry into thecells. For specific processes that must take place, such as cell recognition, endocytosis, cytosolic trafficking, endosomal escape, dissociation of complex, efficient nuclear uptake and gene expression, severalspecific molecules are attached into gene delivery devices enabling targeting, fusion with the membrane, changes in the microenviroment or in other way affects cellular function for successful transgenic expression. Combinations of viral and nonviral components are hybrid vectors, which are safer as viral and more efficient as nonviral vectors.

24.06.2015