Callus-mediatede embryogenesis of leaf celery (Apium graveolens L. var. secalinum Alef.) as a response to malt, peptone and yeast extracts application

english

2025

volume 121, issue 4

Apium graveolens L. var. secalinum   biotic elicitors   callus   embryogenesis   histological investigation

Univerza v Ljubljani, Biotehniška fakulteta
(Obvezni izvod spletne publikacije)

Long times ago, celery (Apium graveolens) of Apiaceae family is well-known traditional plant that possesses eminent therapeutic characteristics. The current work aims to develop an efficient protocol for celery embryogenesis via indirect callus cell cultures approach using biotic elicitors (malt, peptone and yeast) at different concentrations (1.5, 2.5, 3.5 g\l) of each. The optimal explant to induce calli was the stem explant on the best selected medium (0.5 mg/l 2, 4-D). Fresh weight and growth value of celery stem calli were progressively augmented even reach out to the highest values after 8 weeks of culture, whereas the highest growth rate was achieved after 5 weeks of culture. Malt extract (1.5 g/l) performed efficiently as the best biotic elicitor for superior value of callus fresh weight. Similarly, growth rate and growth value were enhanced to 1.2±0.009E-fg/day and 12.1±0.12F-e, respectively. Throughout the histological investigation, the anatomical structure for all elicited treatments of celery stem calli exhibited more potent efficiency of malt than peptone for embryogenesis development then the yeast extract. Meanwhile, free elicitors’ MS-medium stopped to the stage of globular embryo formation. This work authenticated the types and concentrations potency of elicitors for callusing augmentation and somatic embryogenesis development of Apium graveolens.

19.01.2026